Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats
Purpose. Hesperetin and naringenin are naturally common flavonoids reported to have antioxidative effects. This study was performed to investigate whether either hesperetin or naringenin has a protective effect against apoptosis on retinal ischemia/reperfusion (I/R) injury. Methods. Retinal I/R was...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/797824 |
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| author | Selcuk Kara Baran Gencer Turan Karaca Hasan Ali Tufan Sedat Arikan Ismail Ersan Ihsan Karaboga Volkan Hanci |
| author_facet | Selcuk Kara Baran Gencer Turan Karaca Hasan Ali Tufan Sedat Arikan Ismail Ersan Ihsan Karaboga Volkan Hanci |
| author_sort | Selcuk Kara |
| collection | DOAJ |
| description | Purpose. Hesperetin and naringenin are naturally common flavonoids reported to have antioxidative effects. This study was performed to investigate whether either hesperetin or naringenin has a protective effect against apoptosis on retinal ischemia/reperfusion (I/R) injury. Methods. Retinal I/R was induced by increasing the intraocular pressure to 150 mmHg for 60 minutes. Thirty-three male Wistar albino rats were randomised into 5 groups named control, I/R + sham, I/R + solvent (DMSO), I/R + hesperetin, and I/R + naringenin. Animals were given either hesperetin, naringenin, or the solvent intraperitoneally immediately following reperfusion. Thickness of retinal layers and retinal cell apoptosis were detected by histological analysis, tunel assay, and immunohistochemistry assay. Results. Hesperetin and naringenin attenuated the I/R-induced apoptosis of retinal cells in the inner and outer nuclear cells of the rat retina. Retinal layer thickness of the naringenin treatment group was significantly thicker than that of the hesperetin, sham, and solvent groups (P<0.05). Conclusions. Hesperetin and naringenin can prevent harmful effects induced by I/R injury in the rat retina by inhibiting apoptosis of retinal cells, which suggests that those flavanones have a therapeutic potential for the protection of ocular ischemic diseases. |
| format | Article |
| id | doaj-art-507410b6d47a45c69c890ee5e66e69bc |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-507410b6d47a45c69c890ee5e66e69bc2025-08-20T03:36:18ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/797824797824Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in RatsSelcuk Kara0Baran Gencer1Turan Karaca2Hasan Ali Tufan3Sedat Arikan4Ismail Ersan5Ihsan Karaboga6Volkan Hanci7Department of Ophthalmology, Canakkale Onsekiz Mart University, Faculty of Medicine, 17060 Canakkale, TurkeyDepartment of Ophthalmology, Canakkale Onsekiz Mart University, Faculty of Medicine, 17060 Canakkale, TurkeyDepartment of Histology and Embryology, Trakya University, Faculty of Medicine, 22030 Edirne, TurkeyDepartment of Ophthalmology, Canakkale Onsekiz Mart University, Faculty of Medicine, 17060 Canakkale, TurkeyDepartment of Ophthalmology, Canakkale Onsekiz Mart University, Faculty of Medicine, 17060 Canakkale, TurkeyDepartment of Ophthalmology, Canakkale Onsekiz Mart University, Faculty of Medicine, 17060 Canakkale, TurkeyDepartment of Histology and Embryology, Trakya University, Faculty of Medicine, 22030 Edirne, TurkeyDeparment of Anesthesiology, Dokuz Eylül University, Faculty of Medicine, 35100 Izmir, TurkeyPurpose. Hesperetin and naringenin are naturally common flavonoids reported to have antioxidative effects. This study was performed to investigate whether either hesperetin or naringenin has a protective effect against apoptosis on retinal ischemia/reperfusion (I/R) injury. Methods. Retinal I/R was induced by increasing the intraocular pressure to 150 mmHg for 60 minutes. Thirty-three male Wistar albino rats were randomised into 5 groups named control, I/R + sham, I/R + solvent (DMSO), I/R + hesperetin, and I/R + naringenin. Animals were given either hesperetin, naringenin, or the solvent intraperitoneally immediately following reperfusion. Thickness of retinal layers and retinal cell apoptosis were detected by histological analysis, tunel assay, and immunohistochemistry assay. Results. Hesperetin and naringenin attenuated the I/R-induced apoptosis of retinal cells in the inner and outer nuclear cells of the rat retina. Retinal layer thickness of the naringenin treatment group was significantly thicker than that of the hesperetin, sham, and solvent groups (P<0.05). Conclusions. Hesperetin and naringenin can prevent harmful effects induced by I/R injury in the rat retina by inhibiting apoptosis of retinal cells, which suggests that those flavanones have a therapeutic potential for the protection of ocular ischemic diseases.http://dx.doi.org/10.1155/2014/797824 |
| spellingShingle | Selcuk Kara Baran Gencer Turan Karaca Hasan Ali Tufan Sedat Arikan Ismail Ersan Ihsan Karaboga Volkan Hanci Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats The Scientific World Journal |
| title | Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats |
| title_full | Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats |
| title_fullStr | Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats |
| title_full_unstemmed | Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats |
| title_short | Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats |
| title_sort | protective effect of hesperetin and naringenin against apoptosis in ischemia reperfusion induced retinal injury in rats |
| url | http://dx.doi.org/10.1155/2014/797824 |
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