Comparison of Rumen Fluid pH by Continuous Telemetry System and Bench pH Meter in Sheep with Different Ranges of Ruminal pH

We aimed to compare the measurements of sheep ruminal pH using a continuous telemetry system or a bench pH meter using sheep with different degrees of ruminal pH. Ruminal lactic acidosis was induced in nine adult crossbred Santa Ines sheep by the administration of 15 g of sucrose per kg/BW. Samples...

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Main Authors: Leonardo F. Reis, Antonio H. H. Minervino, Carolina A. S. C. Araújo, Rejane S. Sousa, Francisco L. C. Oliveira, Frederico A. M. L. Rodrigues, Enoch B. S. Meira-Júnior, Raimundo A. Barrêto-Júnior, Clara S. Mori, Enrico L. Ortolani
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2014/195782
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author Leonardo F. Reis
Antonio H. H. Minervino
Carolina A. S. C. Araújo
Rejane S. Sousa
Francisco L. C. Oliveira
Frederico A. M. L. Rodrigues
Enoch B. S. Meira-Júnior
Raimundo A. Barrêto-Júnior
Clara S. Mori
Enrico L. Ortolani
author_facet Leonardo F. Reis
Antonio H. H. Minervino
Carolina A. S. C. Araújo
Rejane S. Sousa
Francisco L. C. Oliveira
Frederico A. M. L. Rodrigues
Enoch B. S. Meira-Júnior
Raimundo A. Barrêto-Júnior
Clara S. Mori
Enrico L. Ortolani
author_sort Leonardo F. Reis
collection DOAJ
description We aimed to compare the measurements of sheep ruminal pH using a continuous telemetry system or a bench pH meter using sheep with different degrees of ruminal pH. Ruminal lactic acidosis was induced in nine adult crossbred Santa Ines sheep by the administration of 15 g of sucrose per kg/BW. Samples of rumen fluid were collected at the baseline, before the induction of acidosis (T0) and at six, 12, 18, 24, 48, and 72 hours after the induction for pH measurement using a bench pH meter. During this 72-hour period, all animals had electrodes for the continuous measurement of pH. The results were compared using the Bland-Altman analysis of agreement, Pearson coefficients of correlation and determination, and paired analysis of variance with Student’s t-test. The measurement methods presented a strong correlation (r=0.94, P<0.05) but the rumen pH that was measured continuously using a telemetry system resulted in lower values than the bench pH meter (overall mean of 5.38 and 5.48, resp., P=0.0001). The telemetry system was able to detect smaller changes in rumen fluid pH and was more accurate in diagnosing both subacute ruminal lactic acidosis and acute ruminal lactic acidosis in sheep.
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spelling doaj-art-506bbddabe954ede8bae45257f4bcfbd2025-08-20T03:36:15ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/195782195782Comparison of Rumen Fluid pH by Continuous Telemetry System and Bench pH Meter in Sheep with Different Ranges of Ruminal pHLeonardo F. Reis0Antonio H. H. Minervino1Carolina A. S. C. Araújo2Rejane S. Sousa3Francisco L. C. Oliveira4Frederico A. M. L. Rodrigues5Enoch B. S. Meira-Júnior6Raimundo A. Barrêto-Júnior7Clara S. Mori8Enrico L. Ortolani9Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, BrazilInstitute of Biodiversity and Forest, Federal University of Western Pará, Avenida Vera Paz S/N, Salé, 68000-000 Santarém, PA, BrazilDepartment of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartment of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartment of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartment of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartment of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartment of Animal Science, Federal Rural University of the Semiarid Region, Avenida Francisco Mota, s/n Bairro Presidente Costa e Silva, 59625-900 Mossoró, RN, BrazilDepartment of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, BrazilDepartment of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, BrazilWe aimed to compare the measurements of sheep ruminal pH using a continuous telemetry system or a bench pH meter using sheep with different degrees of ruminal pH. Ruminal lactic acidosis was induced in nine adult crossbred Santa Ines sheep by the administration of 15 g of sucrose per kg/BW. Samples of rumen fluid were collected at the baseline, before the induction of acidosis (T0) and at six, 12, 18, 24, 48, and 72 hours after the induction for pH measurement using a bench pH meter. During this 72-hour period, all animals had electrodes for the continuous measurement of pH. The results were compared using the Bland-Altman analysis of agreement, Pearson coefficients of correlation and determination, and paired analysis of variance with Student’s t-test. The measurement methods presented a strong correlation (r=0.94, P<0.05) but the rumen pH that was measured continuously using a telemetry system resulted in lower values than the bench pH meter (overall mean of 5.38 and 5.48, resp., P=0.0001). The telemetry system was able to detect smaller changes in rumen fluid pH and was more accurate in diagnosing both subacute ruminal lactic acidosis and acute ruminal lactic acidosis in sheep.http://dx.doi.org/10.1155/2014/195782
spellingShingle Leonardo F. Reis
Antonio H. H. Minervino
Carolina A. S. C. Araújo
Rejane S. Sousa
Francisco L. C. Oliveira
Frederico A. M. L. Rodrigues
Enoch B. S. Meira-Júnior
Raimundo A. Barrêto-Júnior
Clara S. Mori
Enrico L. Ortolani
Comparison of Rumen Fluid pH by Continuous Telemetry System and Bench pH Meter in Sheep with Different Ranges of Ruminal pH
The Scientific World Journal
title Comparison of Rumen Fluid pH by Continuous Telemetry System and Bench pH Meter in Sheep with Different Ranges of Ruminal pH
title_full Comparison of Rumen Fluid pH by Continuous Telemetry System and Bench pH Meter in Sheep with Different Ranges of Ruminal pH
title_fullStr Comparison of Rumen Fluid pH by Continuous Telemetry System and Bench pH Meter in Sheep with Different Ranges of Ruminal pH
title_full_unstemmed Comparison of Rumen Fluid pH by Continuous Telemetry System and Bench pH Meter in Sheep with Different Ranges of Ruminal pH
title_short Comparison of Rumen Fluid pH by Continuous Telemetry System and Bench pH Meter in Sheep with Different Ranges of Ruminal pH
title_sort comparison of rumen fluid ph by continuous telemetry system and bench ph meter in sheep with different ranges of ruminal ph
url http://dx.doi.org/10.1155/2014/195782
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