Systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers.
<h4>Unlabelled</h4>The sublingual route has been proposed as a needle-free option to induce systemic and mucosal immune protection against viral infections. In a translational study of systemic and mucosal humoral immune responses to sublingual or systemically administered viral antigens...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033736&type=printable |
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| author | Zhiming Huo Sara L Bissett Raphaela Giemza Simon Beddows Clarissa Oeser David J M Lewis |
| author_facet | Zhiming Huo Sara L Bissett Raphaela Giemza Simon Beddows Clarissa Oeser David J M Lewis |
| author_sort | Zhiming Huo |
| collection | DOAJ |
| description | <h4>Unlabelled</h4>The sublingual route has been proposed as a needle-free option to induce systemic and mucosal immune protection against viral infections. In a translational study of systemic and mucosal humoral immune responses to sublingual or systemically administered viral antigens, eighteen healthy female volunteers aged 19-31 years received three immunizations with a quadravalent Human Papilloma Virus vaccine at 0, 4 and 16 weeks as sublingual drops (SL, n = 12) or intramuscular injection (IM, n = 6). IM antigen delivery induced or boosted HPV-specific serum IgG and pseudovirus-neutralizing antibodies, HPV-specific cervical and vaginal IgG, and elicited circulating IgG and IgA antibody secreting cells. SL antigens induced ~38-fold lower serum and ~2-fold lower cervical/vaginal IgG than IM delivery, and induced or boosted serum virus neutralizing antibody in only 3/12 subjects. Neither route reproducibly induced HPV-specific mucosal IgA. Alternative delivery systems and adjuvants will be required to enhance and evaluate immune responses following sublingual immunization in humans.<h4>Trial registration</h4>ClinicalTrials.govNCT00949572. |
| format | Article |
| id | doaj-art-5067e504fe144bef9a60efdb865aa91f |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5067e504fe144bef9a60efdb865aa91f2025-08-20T02:35:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3373610.1371/journal.pone.0033736Systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers.Zhiming HuoSara L BissettRaphaela GiemzaSimon BeddowsClarissa OeserDavid J M Lewis<h4>Unlabelled</h4>The sublingual route has been proposed as a needle-free option to induce systemic and mucosal immune protection against viral infections. In a translational study of systemic and mucosal humoral immune responses to sublingual or systemically administered viral antigens, eighteen healthy female volunteers aged 19-31 years received three immunizations with a quadravalent Human Papilloma Virus vaccine at 0, 4 and 16 weeks as sublingual drops (SL, n = 12) or intramuscular injection (IM, n = 6). IM antigen delivery induced or boosted HPV-specific serum IgG and pseudovirus-neutralizing antibodies, HPV-specific cervical and vaginal IgG, and elicited circulating IgG and IgA antibody secreting cells. SL antigens induced ~38-fold lower serum and ~2-fold lower cervical/vaginal IgG than IM delivery, and induced or boosted serum virus neutralizing antibody in only 3/12 subjects. Neither route reproducibly induced HPV-specific mucosal IgA. Alternative delivery systems and adjuvants will be required to enhance and evaluate immune responses following sublingual immunization in humans.<h4>Trial registration</h4>ClinicalTrials.govNCT00949572.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033736&type=printable |
| spellingShingle | Zhiming Huo Sara L Bissett Raphaela Giemza Simon Beddows Clarissa Oeser David J M Lewis Systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers. PLoS ONE |
| title | Systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers. |
| title_full | Systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers. |
| title_fullStr | Systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers. |
| title_full_unstemmed | Systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers. |
| title_short | Systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers. |
| title_sort | systemic and mucosal immune responses to sublingual or intramuscular human papilloma virus antigens in healthy female volunteers |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033736&type=printable |
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