Cellular Imaging of Inflammation in Atherosclerosis Using Magnetofluorescent Nanomaterials
Objective: Magnetofluorescent nanoparticles (MFNPs) offer the ability to image cellular inflammation in vivo. To better understand their cellular targeting and imaging capabilities in atherosclerosis, we investigated prototypical dextran-coated near-infrared fluorescent MFNPs in the apolipoprotein E...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2006-04-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00009 |
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| _version_ | 1841561769723559936 |
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| author | Farouc A. Jaffer Matthias Nahrendorf David Sosnovik Kimberly A. Kelly Elena Aikawa Ralph Weissleder |
| author_facet | Farouc A. Jaffer Matthias Nahrendorf David Sosnovik Kimberly A. Kelly Elena Aikawa Ralph Weissleder |
| author_sort | Farouc A. Jaffer |
| collection | DOAJ |
| description | Objective: Magnetofluorescent nanoparticles (MFNPs) offer the ability to image cellular inflammation in vivo. To better understand their cellular targeting and imaging capabilities in atherosclerosis, we investigated prototypical dextran-coated near-infrared fluorescent MFNPs in the apolipoprotein E-deficient (apo E−/−) mouse model. Methods and Results: In vitro MFNP uptake was highest in activated murine macrophages ( p < .001). Apo E−/− mice ( n = 11) were next injected with the MFNP (15 mg/kg iron) or saline. In vivo magnetic resonance imaging (MRI) demonstrated strong plaque enhancement by the MFNPs ( p < .001 vs. saline), which was confirmed by multimodality ex vivo MRI and fluorescence reflectance imaging. On fluorescence microscopy, MFNPs were found in cellular-rich areas of atheroma and colocalized with immunofluorescent macrophages over endothelial cells and smooth muscle cells ( p < .001). Conclusions: Here we show that (1) the in vitro and in vivo cellular distribution of atherosclerosis-targeted MFNPs can be quantified by using fluorescence imaging methods; (2) in atherosclerosis, dextranated MFNPs preferentially target macrophages; and (3) MFNP deposition in murine atheroma can be noninvasively detected by in vivo MRI. This study thus provides a foundation for using MFNPs to image genetic and/or pharmacological perturbations of cellular inflammation in experimental atherosclerosis and for the future development of novel targeted nanomaterials for atherosclerosis. |
| format | Article |
| id | doaj-art-50626fe742e9471b89b3f4dd40bbd268 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2006-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-50626fe742e9471b89b3f4dd40bbd2682025-01-03T01:25:02ZengSAGE PublishingMolecular Imaging1536-01212006-04-01510.2310/7290.2006.0000910.2310_7290.2006.00009Cellular Imaging of Inflammation in Atherosclerosis Using Magnetofluorescent NanomaterialsFarouc A. JafferMatthias NahrendorfDavid SosnovikKimberly A. KellyElena AikawaRalph WeisslederObjective: Magnetofluorescent nanoparticles (MFNPs) offer the ability to image cellular inflammation in vivo. To better understand their cellular targeting and imaging capabilities in atherosclerosis, we investigated prototypical dextran-coated near-infrared fluorescent MFNPs in the apolipoprotein E-deficient (apo E−/−) mouse model. Methods and Results: In vitro MFNP uptake was highest in activated murine macrophages ( p < .001). Apo E−/− mice ( n = 11) were next injected with the MFNP (15 mg/kg iron) or saline. In vivo magnetic resonance imaging (MRI) demonstrated strong plaque enhancement by the MFNPs ( p < .001 vs. saline), which was confirmed by multimodality ex vivo MRI and fluorescence reflectance imaging. On fluorescence microscopy, MFNPs were found in cellular-rich areas of atheroma and colocalized with immunofluorescent macrophages over endothelial cells and smooth muscle cells ( p < .001). Conclusions: Here we show that (1) the in vitro and in vivo cellular distribution of atherosclerosis-targeted MFNPs can be quantified by using fluorescence imaging methods; (2) in atherosclerosis, dextranated MFNPs preferentially target macrophages; and (3) MFNP deposition in murine atheroma can be noninvasively detected by in vivo MRI. This study thus provides a foundation for using MFNPs to image genetic and/or pharmacological perturbations of cellular inflammation in experimental atherosclerosis and for the future development of novel targeted nanomaterials for atherosclerosis.https://doi.org/10.2310/7290.2006.00009 |
| spellingShingle | Farouc A. Jaffer Matthias Nahrendorf David Sosnovik Kimberly A. Kelly Elena Aikawa Ralph Weissleder Cellular Imaging of Inflammation in Atherosclerosis Using Magnetofluorescent Nanomaterials Molecular Imaging |
| title | Cellular Imaging of Inflammation in Atherosclerosis Using Magnetofluorescent Nanomaterials |
| title_full | Cellular Imaging of Inflammation in Atherosclerosis Using Magnetofluorescent Nanomaterials |
| title_fullStr | Cellular Imaging of Inflammation in Atherosclerosis Using Magnetofluorescent Nanomaterials |
| title_full_unstemmed | Cellular Imaging of Inflammation in Atherosclerosis Using Magnetofluorescent Nanomaterials |
| title_short | Cellular Imaging of Inflammation in Atherosclerosis Using Magnetofluorescent Nanomaterials |
| title_sort | cellular imaging of inflammation in atherosclerosis using magnetofluorescent nanomaterials |
| url | https://doi.org/10.2310/7290.2006.00009 |
| work_keys_str_mv | AT faroucajaffer cellularimagingofinflammationinatherosclerosisusingmagnetofluorescentnanomaterials AT matthiasnahrendorf cellularimagingofinflammationinatherosclerosisusingmagnetofluorescentnanomaterials AT davidsosnovik cellularimagingofinflammationinatherosclerosisusingmagnetofluorescentnanomaterials AT kimberlyakelly cellularimagingofinflammationinatherosclerosisusingmagnetofluorescentnanomaterials AT elenaaikawa cellularimagingofinflammationinatherosclerosisusingmagnetofluorescentnanomaterials AT ralphweissleder cellularimagingofinflammationinatherosclerosisusingmagnetofluorescentnanomaterials |