Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer Screening
Abstract Prostate specific antigen (PSA) is widely used in liquid biopsy of prostate cancer (PCa) but still faces challenges due to the poor specificity. Herein, this study reports a double‐SERS satellite immunoassay, made of an Au–Ag dealloyed intra‐nanogap nanoflower (Au–Ag DINF) with strong SERS...
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Wiley
2025-02-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202411747 |
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| author | Dong Chen Yilin Ma Annan Yang Liping Hu Hanlin Zhou Jun Xu Shanze Chen Dingmeng Nie Weifeng Feng Huaihong Cai Yanguang Cong Jiang Pi Lang Rao Xueqin Huang Pinghua Sun Haibo Zhou |
| author_facet | Dong Chen Yilin Ma Annan Yang Liping Hu Hanlin Zhou Jun Xu Shanze Chen Dingmeng Nie Weifeng Feng Huaihong Cai Yanguang Cong Jiang Pi Lang Rao Xueqin Huang Pinghua Sun Haibo Zhou |
| author_sort | Dong Chen |
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| description | Abstract Prostate specific antigen (PSA) is widely used in liquid biopsy of prostate cancer (PCa) but still faces challenges due to the poor specificity. Herein, this study reports a double‐SERS satellite immunoassay, made of an Au–Ag dealloyed intra‐nanogap nanoflower (Au–Ag DINF) with strong SERS signals and Au magnetic nanoparticles (AuMNPs) with magnetic capture and SERS amplification, for sensing multiple PSA (free PSA (fPSA), complexed PSA (cPSA) and [‐2]proPSA (p2PSA)) toward potential PCa screening. Unlike the previous studies focus on the tPSA and fPSA/tPSA ratio (f/t PSA%), this work introduces a multiple PSA‐mediated Prostate Health Index (PHI) assay with significantly increased the predictive accuracy and specificity of PCa, especially the patients with a tPSA level in the “diagnostic gray zone”. Practical analysis of clinical samples demonstrated the SERS immunoassay is capable of identifying the PCa subjects from healthy candidates (N = 13), and monitoring PCa patients before and after surgery (N = 5), as well as screening suspicious ones who are in the “gray zone” (N = 10). Overall, benefiting from the PSA‐responsive satellite immuno‐nanoassemble strategy, dual‐SERS magnification effect, and multiple PSA‐based PHI assessment, this proposes bioassay held enormous potential for the early diagnosis, screening, monitoring, and prognosis of PCa. |
| format | Article |
| id | doaj-art-5060015251944cf596d2e7895088d41f |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-5060015251944cf596d2e7895088d41f2025-02-04T13:14:54ZengWileyAdvanced Science2198-38442025-02-01125n/an/a10.1002/advs.202411747Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer ScreeningDong Chen0Yilin Ma1Annan Yang2Liping Hu3Hanlin Zhou4Jun Xu5Shanze Chen6Dingmeng Nie7Weifeng Feng8Huaihong Cai9Yanguang Cong10Jiang Pi11Lang Rao12Xueqin Huang13Pinghua Sun14Haibo Zhou15Institute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaInstitute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaInstitute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaInstitute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaInstitute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaInstitute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaInstitute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaInstitute of Chemical Biology Shenzhen Bay Laboratory Shenzhen 518132 ChinaThe First Affiliated Hospital of Jinan University Guangzhou 510632 ChinaCollege of Chemistry and Materials Science Jinan University Guangzhou 510632 ChinaGuangdong Provincial Key Laboratory of Medical Molecular Diagnostics The First Dongguan Affiliated Hospital School of Medical Technology Guangdong Medical University Dongguan 523000 ChinaGuangdong Provincial Key Laboratory of Medical Molecular Diagnostics The First Dongguan Affiliated Hospital School of Medical Technology Guangdong Medical University Dongguan 523000 ChinaInstitute of Chemical Biology Shenzhen Bay Laboratory Shenzhen 518132 ChinaGuangdong Provincial Key Laboratory of Medical Molecular Diagnostics The First Dongguan Affiliated Hospital School of Medical Technology Guangdong Medical University Dongguan 523000 ChinaInstitute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaInstitute for Safflower Industry Research Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education) School of Pharmacy Shihezi University Shihezi 832003 ChinaAbstract Prostate specific antigen (PSA) is widely used in liquid biopsy of prostate cancer (PCa) but still faces challenges due to the poor specificity. Herein, this study reports a double‐SERS satellite immunoassay, made of an Au–Ag dealloyed intra‐nanogap nanoflower (Au–Ag DINF) with strong SERS signals and Au magnetic nanoparticles (AuMNPs) with magnetic capture and SERS amplification, for sensing multiple PSA (free PSA (fPSA), complexed PSA (cPSA) and [‐2]proPSA (p2PSA)) toward potential PCa screening. Unlike the previous studies focus on the tPSA and fPSA/tPSA ratio (f/t PSA%), this work introduces a multiple PSA‐mediated Prostate Health Index (PHI) assay with significantly increased the predictive accuracy and specificity of PCa, especially the patients with a tPSA level in the “diagnostic gray zone”. Practical analysis of clinical samples demonstrated the SERS immunoassay is capable of identifying the PCa subjects from healthy candidates (N = 13), and monitoring PCa patients before and after surgery (N = 5), as well as screening suspicious ones who are in the “gray zone” (N = 10). Overall, benefiting from the PSA‐responsive satellite immuno‐nanoassemble strategy, dual‐SERS magnification effect, and multiple PSA‐based PHI assessment, this proposes bioassay held enormous potential for the early diagnosis, screening, monitoring, and prognosis of PCa.https://doi.org/10.1002/advs.202411747intra‐nanogap particlesPCa screeningprostate health indexprostate specific antigensurface‐enhanced raman scattering |
| spellingShingle | Dong Chen Yilin Ma Annan Yang Liping Hu Hanlin Zhou Jun Xu Shanze Chen Dingmeng Nie Weifeng Feng Huaihong Cai Yanguang Cong Jiang Pi Lang Rao Xueqin Huang Pinghua Sun Haibo Zhou Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer Screening Advanced Science intra‐nanogap particles PCa screening prostate health index prostate specific antigen surface‐enhanced raman scattering |
| title | Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer Screening |
| title_full | Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer Screening |
| title_fullStr | Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer Screening |
| title_full_unstemmed | Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer Screening |
| title_short | Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer Screening |
| title_sort | dual enhanced sers satellite immuno nanocomplex for multiple psa mediated phi assay toward clinical prostate cancer screening |
| topic | intra‐nanogap particles PCa screening prostate health index prostate specific antigen surface‐enhanced raman scattering |
| url | https://doi.org/10.1002/advs.202411747 |
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