The hydroethanolic extract of leaves of Laelia furfuracea inhibits blood coagulation and platelet aggregation
Background: Laelia furfuracea is an orchid with anticoagulant properties; however, it is unknown which coagulation factors are inhibited and whether the plant has antiplatelet activity. Questions: Does the hydroethanolic extract of leaves of L. furfuracea inhibit coagulation factor activity and p...
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| Format: | Article |
| Language: | English |
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Sociedad Botánica de México, A. C.
2025-06-01
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| Series: | Botanical Sciences |
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| Online Access: | https://botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3645 |
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| author | Abimael López-Pérez Luicita Lagunez-Rivera Rodolfo Solano Edgar García-Sánchez Gabriela Soledad Barragán-Zárate Berenice Fernández-Rojas Abraham Majluf-Cruz Jesús Hernández-Juárez |
| author_facet | Abimael López-Pérez Luicita Lagunez-Rivera Rodolfo Solano Edgar García-Sánchez Gabriela Soledad Barragán-Zárate Berenice Fernández-Rojas Abraham Majluf-Cruz Jesús Hernández-Juárez |
| author_sort | Abimael López-Pérez |
| collection | DOAJ |
| description |
Background: Laelia furfuracea is an orchid with anticoagulant properties; however, it is unknown which coagulation factors are inhibited and whether the plant has antiplatelet activity.
Questions: Does the hydroethanolic extract of leaves of L. furfuracea inhibit coagulation factor activity and platelet aggregation?
Studied species: Laelia furfuracea.
Study site and dates: Plant material was collected in April 2023 in Santo Domingo Yanhuitlán, Oaxaca, Mexico.
Methods: The anticoagulant and antiplatelet effects of the extracts of L. furfuracea were evaluated in samples from patients with thrombosis through coagulometric assays and light transmission aggregometry.
Results: The extract of L. furfuracea inhibited the activity of factors II, V, VIII, and XI in a concentration-dependent manner. The highest extract concentration, 14.0 mg/mL, inhibited about 60 % of factors II and V activity, but ˃ 95 % of factors VIII and XI. Moreover, at a 1.0 mg/mL concentration, the extract inhibited platelet aggregation induced by ADP, thrombin, and epinephrine in 85, 97, and 83 %, respectively.
Conclusions: It was demonstrated that the orchid extract inhibits both the activity of factors II, V, VIII, XI, and thrombin-, ADP-, and epinephrine-induced platelet aggregation. Polyphenols stand out among the possible bioactive compounds of the orchid with anticoagulant and/or antiplatelet activity.
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| format | Article |
| id | doaj-art-505ebdf4b79340e2a9f8454be62f2c2c |
| institution | Kabale University |
| issn | 2007-4298 2007-4476 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Sociedad Botánica de México, A. C. |
| record_format | Article |
| series | Botanical Sciences |
| spelling | doaj-art-505ebdf4b79340e2a9f8454be62f2c2c2025-08-20T03:44:54ZengSociedad Botánica de México, A. C.Botanical Sciences2007-42982007-44762025-06-01103310.17129/botsci.3645The hydroethanolic extract of leaves of Laelia furfuracea inhibits blood coagulation and platelet aggregationAbimael López-Pérez0https://orcid.org/0009-0004-9556-6169Luicita Lagunez-Rivera1https://orcid.org/0000-0001-9870-971XRodolfo Solano2https://orcid.org/0000-0002-0355-5496Edgar García-Sánchez3https://orcid.org/0000-0001-6183-957XGabriela Soledad Barragán-Zárate4https://orcid.org/0000-0001-7046-4001Berenice Fernández-Rojas5https://orcid.org/0000-0002-4679-4032Abraham Majluf-Cruz6https://orcid.org/0000-0002-7697-5790Jesús Hernández-Juárez7https://orcid.org/0000-0002-5505-1097Laboratorio de Extracción y Análisis de Productos Naturales Vegetales, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Oaxaca, Instituto Politécnico Nacional, OaxacaLaboratorio de Extracción y Análisis de Productos Naturales Vegetales, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Oaxaca, Instituto Politécnico Nacional, OaxacaLaboratorio de Extracción y Análisis de Productos Naturales Vegetales, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Oaxaca, Instituto Politécnico Nacional, OaxacaSECIHTI-Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Oaxaca, Instituto Politécnico Nacional, OaxacaLaboratorio de Extracción y Análisis de Productos Naturales Vegetales, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Oaxaca, Instituto Politécnico Nacional, OaxacaDepartamento de Fisiología, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de MéxicoUnidad de Investigación Médica en Trombosis, Hemostasia y Aterogénesis, Instituto Mexicano del Seguro Social, Ciudad de MéxicoSECIHTI-Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Oaxaca, Instituto Politécnico Nacional, Oaxaca Background: Laelia furfuracea is an orchid with anticoagulant properties; however, it is unknown which coagulation factors are inhibited and whether the plant has antiplatelet activity. Questions: Does the hydroethanolic extract of leaves of L. furfuracea inhibit coagulation factor activity and platelet aggregation? Studied species: Laelia furfuracea. Study site and dates: Plant material was collected in April 2023 in Santo Domingo Yanhuitlán, Oaxaca, Mexico. Methods: The anticoagulant and antiplatelet effects of the extracts of L. furfuracea were evaluated in samples from patients with thrombosis through coagulometric assays and light transmission aggregometry. Results: The extract of L. furfuracea inhibited the activity of factors II, V, VIII, and XI in a concentration-dependent manner. The highest extract concentration, 14.0 mg/mL, inhibited about 60 % of factors II and V activity, but ˃ 95 % of factors VIII and XI. Moreover, at a 1.0 mg/mL concentration, the extract inhibited platelet aggregation induced by ADP, thrombin, and epinephrine in 85, 97, and 83 %, respectively. Conclusions: It was demonstrated that the orchid extract inhibits both the activity of factors II, V, VIII, XI, and thrombin-, ADP-, and epinephrine-induced platelet aggregation. Polyphenols stand out among the possible bioactive compounds of the orchid with anticoagulant and/or antiplatelet activity. https://botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3645coagulation factorsantiplateletnatural productsorchidthrombosis |
| spellingShingle | Abimael López-Pérez Luicita Lagunez-Rivera Rodolfo Solano Edgar García-Sánchez Gabriela Soledad Barragán-Zárate Berenice Fernández-Rojas Abraham Majluf-Cruz Jesús Hernández-Juárez The hydroethanolic extract of leaves of Laelia furfuracea inhibits blood coagulation and platelet aggregation Botanical Sciences coagulation factors antiplatelet natural products orchid thrombosis |
| title | The hydroethanolic extract of leaves of Laelia furfuracea inhibits blood coagulation and platelet aggregation |
| title_full | The hydroethanolic extract of leaves of Laelia furfuracea inhibits blood coagulation and platelet aggregation |
| title_fullStr | The hydroethanolic extract of leaves of Laelia furfuracea inhibits blood coagulation and platelet aggregation |
| title_full_unstemmed | The hydroethanolic extract of leaves of Laelia furfuracea inhibits blood coagulation and platelet aggregation |
| title_short | The hydroethanolic extract of leaves of Laelia furfuracea inhibits blood coagulation and platelet aggregation |
| title_sort | hydroethanolic extract of leaves of laelia furfuracea inhibits blood coagulation and platelet aggregation |
| topic | coagulation factors antiplatelet natural products orchid thrombosis |
| url | https://botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3645 |
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