Gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD mice

Abstract Drug tolerance is clinically common but its mechanism is unclear. Previous studies found RSV tolerance in ALD treatment. This study explores mechanisms involving gut bacteria and host factors. Male C57BL/6J mice were induced into ALD with the Lieber-DeCarli alcohol diet, then ALD-RSV group...

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Main Authors: Song-xia Zhang, Fang-fang Hu, Huan Chen, Jing Guo, Zhong-wen Xiang, Xin Ding, Meng-ling Ye, Wen-li Ye, Jun-hong Chen, Xin Wang, Wen-jing Han, Hong-hao Zhou, Wei Zhang, Yun Huang, Lie-lin Wu, Guang-hui Lian, Yao Chen
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:npj Biofilms and Microbiomes
Online Access:https://doi.org/10.1038/s41522-025-00766-y
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author Song-xia Zhang
Fang-fang Hu
Huan Chen
Jing Guo
Zhong-wen Xiang
Xin Ding
Meng-ling Ye
Wen-li Ye
Jun-hong Chen
Xin Wang
Wen-jing Han
Hong-hao Zhou
Wei Zhang
Yun Huang
Lie-lin Wu
Guang-hui Lian
Yao Chen
author_facet Song-xia Zhang
Fang-fang Hu
Huan Chen
Jing Guo
Zhong-wen Xiang
Xin Ding
Meng-ling Ye
Wen-li Ye
Jun-hong Chen
Xin Wang
Wen-jing Han
Hong-hao Zhou
Wei Zhang
Yun Huang
Lie-lin Wu
Guang-hui Lian
Yao Chen
author_sort Song-xia Zhang
collection DOAJ
description Abstract Drug tolerance is clinically common but its mechanism is unclear. Previous studies found RSV tolerance in ALD treatment. This study explores mechanisms involving gut bacteria and host factors. Male C57BL/6J mice were induced into ALD with the Lieber-DeCarli alcohol diet, then ALD-RSV group mice were gavaged RSV (150 mg/kg/day) for 5 weeks. Throughout the experiment, ALD and ALD-RSV mice were on the Lieber-DeCarli alcohol diet, while the Vehicle group received a control diet. By week 5, tolerance to RSV efficacy emerged, with markedly reduced RSV exposure at the final dose. ALD-RSV group exhibited increased levels of Eggerthella lenta (E.lent), which metabolizes RSV into dihydroresveratrol (DHR). Chronic RSV treatment upregulated UGT1A1, the enzyme converting RSV into its primary metabolite, trans-resveratrol-3-O-β-D-glucuronide (R3G). The synergy between gut bacteria and host factors enhances RSV metabolism in ALD mice, driving tolerance and offering insights into other clinical drugs tolerance mechanisms.
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series npj Biofilms and Microbiomes
spelling doaj-art-505d133ff6724f2d9d96857d4f31d4412025-08-20T03:46:07ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-07-0111111410.1038/s41522-025-00766-yGut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD miceSong-xia Zhang0Fang-fang Hu1Huan Chen2Jing Guo3Zhong-wen Xiang4Xin Ding5Meng-ling Ye6Wen-li Ye7Jun-hong Chen8Xin Wang9Wen-jing Han10Hong-hao Zhou11Wei Zhang12Yun Huang13Lie-lin Wu14Guang-hui Lian15Yao Chen16Department of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityDepartment of Hepatobiliary Surgery, Xiangya Hospital, Central South UniversityDepartment of organ transplantation, Xiangya Hospital, Central South UniversityDepartment of gastroenterology, Xiangya Hospital, Central South UniversityDepartment of Clinical Pharmacology, Xiangya Hospital, Central South UniversityAbstract Drug tolerance is clinically common but its mechanism is unclear. Previous studies found RSV tolerance in ALD treatment. This study explores mechanisms involving gut bacteria and host factors. Male C57BL/6J mice were induced into ALD with the Lieber-DeCarli alcohol diet, then ALD-RSV group mice were gavaged RSV (150 mg/kg/day) for 5 weeks. Throughout the experiment, ALD and ALD-RSV mice were on the Lieber-DeCarli alcohol diet, while the Vehicle group received a control diet. By week 5, tolerance to RSV efficacy emerged, with markedly reduced RSV exposure at the final dose. ALD-RSV group exhibited increased levels of Eggerthella lenta (E.lent), which metabolizes RSV into dihydroresveratrol (DHR). Chronic RSV treatment upregulated UGT1A1, the enzyme converting RSV into its primary metabolite, trans-resveratrol-3-O-β-D-glucuronide (R3G). The synergy between gut bacteria and host factors enhances RSV metabolism in ALD mice, driving tolerance and offering insights into other clinical drugs tolerance mechanisms.https://doi.org/10.1038/s41522-025-00766-y
spellingShingle Song-xia Zhang
Fang-fang Hu
Huan Chen
Jing Guo
Zhong-wen Xiang
Xin Ding
Meng-ling Ye
Wen-li Ye
Jun-hong Chen
Xin Wang
Wen-jing Han
Hong-hao Zhou
Wei Zhang
Yun Huang
Lie-lin Wu
Guang-hui Lian
Yao Chen
Gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD mice
npj Biofilms and Microbiomes
title Gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD mice
title_full Gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD mice
title_fullStr Gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD mice
title_full_unstemmed Gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD mice
title_short Gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD mice
title_sort gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ald mice
url https://doi.org/10.1038/s41522-025-00766-y
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