Integrated miRNA–mRNA Profiling of C2C12 Myoblasts Indicates Regulatory Interactions Involved in Proliferation and Differentiation
Myogenesis is a complex biological process regulated by multiple factors. This study systematically revealed the dynamic changes of gene expression and its regulatory network in C2C12 myoblasts during proliferation and differentiation stages by integrating transcriptome and miRNA-omics data. The ana...
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2025-05-01
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| author | Xiaolong Chang Junwu Ma |
| author_facet | Xiaolong Chang Junwu Ma |
| author_sort | Xiaolong Chang |
| collection | DOAJ |
| description | Myogenesis is a complex biological process regulated by multiple factors. This study systematically revealed the dynamic changes of gene expression and its regulatory network in C2C12 myoblasts during proliferation and differentiation stages by integrating transcriptome and miRNA-omics data. The analysis results showed that in the early stage of proliferation, gene expression showed significant fluctuations, and key cell cycle and DNA replication-related genes were closely associated with specific miRNAs (miR-486a-5p, miR-486b-5p, and miR-351-5p), suggesting that these miRNAs play an important role in regulating cell proliferation. In the differentiation stage, the activation of key myogenic transcription factors and signaling pathways, such as MAPK and PI3K-Akt, synergizes with miRNA regulation to promote the myogenic program. In addition, we found that genes such as <i>IGF1</i> and <i>Dio2</i> were continuously upregulated during differentiation, and IGF1 might be regulated by multiple miRNAs during this process. This study provides key molecular insights for a deeper understanding of muscle development and regeneration. |
| format | Article |
| id | doaj-art-505096d701ea46c98c6e4b9c09b179b0 |
| institution | DOAJ |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj-art-505096d701ea46c98c6e4b9c09b179b02025-08-20T03:14:45ZengMDPI AGBiology2079-77372025-05-0114557410.3390/biology14050574Integrated miRNA–mRNA Profiling of C2C12 Myoblasts Indicates Regulatory Interactions Involved in Proliferation and DifferentiationXiaolong Chang0Junwu Ma1National Key Laboratory for Swine Genetic Improvement and Germplasm Innovation, Jiangxi Agricultural University, Nanchang 330045, ChinaNational Key Laboratory for Swine Genetic Improvement and Germplasm Innovation, Jiangxi Agricultural University, Nanchang 330045, ChinaMyogenesis is a complex biological process regulated by multiple factors. This study systematically revealed the dynamic changes of gene expression and its regulatory network in C2C12 myoblasts during proliferation and differentiation stages by integrating transcriptome and miRNA-omics data. The analysis results showed that in the early stage of proliferation, gene expression showed significant fluctuations, and key cell cycle and DNA replication-related genes were closely associated with specific miRNAs (miR-486a-5p, miR-486b-5p, and miR-351-5p), suggesting that these miRNAs play an important role in regulating cell proliferation. In the differentiation stage, the activation of key myogenic transcription factors and signaling pathways, such as MAPK and PI3K-Akt, synergizes with miRNA regulation to promote the myogenic program. In addition, we found that genes such as <i>IGF1</i> and <i>Dio2</i> were continuously upregulated during differentiation, and IGF1 might be regulated by multiple miRNAs during this process. This study provides key molecular insights for a deeper understanding of muscle development and regeneration.https://www.mdpi.com/2079-7737/14/5/574C2C12 myoblastsmiRNAproliferationdifferentiationregulatory network |
| spellingShingle | Xiaolong Chang Junwu Ma Integrated miRNA–mRNA Profiling of C2C12 Myoblasts Indicates Regulatory Interactions Involved in Proliferation and Differentiation Biology C2C12 myoblasts miRNA proliferation differentiation regulatory network |
| title | Integrated miRNA–mRNA Profiling of C2C12 Myoblasts Indicates Regulatory Interactions Involved in Proliferation and Differentiation |
| title_full | Integrated miRNA–mRNA Profiling of C2C12 Myoblasts Indicates Regulatory Interactions Involved in Proliferation and Differentiation |
| title_fullStr | Integrated miRNA–mRNA Profiling of C2C12 Myoblasts Indicates Regulatory Interactions Involved in Proliferation and Differentiation |
| title_full_unstemmed | Integrated miRNA–mRNA Profiling of C2C12 Myoblasts Indicates Regulatory Interactions Involved in Proliferation and Differentiation |
| title_short | Integrated miRNA–mRNA Profiling of C2C12 Myoblasts Indicates Regulatory Interactions Involved in Proliferation and Differentiation |
| title_sort | integrated mirna mrna profiling of c2c12 myoblasts indicates regulatory interactions involved in proliferation and differentiation |
| topic | C2C12 myoblasts miRNA proliferation differentiation regulatory network |
| url | https://www.mdpi.com/2079-7737/14/5/574 |
| work_keys_str_mv | AT xiaolongchang integratedmirnamrnaprofilingofc2c12myoblastsindicatesregulatoryinteractionsinvolvedinproliferationanddifferentiation AT junwuma integratedmirnamrnaprofilingofc2c12myoblastsindicatesregulatoryinteractionsinvolvedinproliferationanddifferentiation |