Simultaneous Estimation and Validation for Determination of Lamivudine and Zidovudine in Human Plasma By LCMS/MS Method
A high performance liquid chromatographic method with mass detection was developed for determination of Lamivudine and Zidovudine in human plasma. Lamivudine and Zidovudine were extracted from an aliquot of human plasma using Solid Phase Extraction technique, and then injected into a liquid chromato...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/716963 |
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| author | C. Parthiban M. Bhagavan Raju M. Sudhakar D. Sathis Kumar |
| author_facet | C. Parthiban M. Bhagavan Raju M. Sudhakar D. Sathis Kumar |
| author_sort | C. Parthiban |
| collection | DOAJ |
| description | A high performance liquid chromatographic method with mass detection was developed for determination of Lamivudine and Zidovudine in human plasma. Lamivudine and Zidovudine were extracted from an aliquot of human plasma using Solid Phase Extraction technique, and then injected into a liquid chromatograph equipped with a tandem mass spectrometry detector; quantitation was done by peak area ratio method. A weighted (1/Conc2) linear regression was performed to determine the concentration of analyte. This method demonstrates acceptable performance and is suitable for the determination of Lamivudine and Zidovudine in human plasma over the range of 25.291 to 4046.621 ng/mL and 23.357 to 4057.l4lng/mL respectively using Solid Phase Extraction Procedure. As all the values obtained were within the limits as specified, the method has been successfully used to analyse the human plasma containing Lamivudine and Zidovudine with good recoveries and proved to be robust. |
| format | Article |
| id | doaj-art-504d6d9820fb462bb9997f0431a1f19e |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-504d6d9820fb462bb9997f0431a1f19e2025-08-20T03:36:18ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-019259860710.1155/2012/716963Simultaneous Estimation and Validation for Determination of Lamivudine and Zidovudine in Human Plasma By LCMS/MS MethodC. Parthiban0M. Bhagavan Raju1M. Sudhakar2D. Sathis Kumar3Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda Dhulapally, Secunderabad, Andhra Pradesh-500 014, IndiaDepartment of Pharmaceutical Chemistry, C M College of Pharmacy, Maisammaguda Dhulapally, Secunderabad, Andhra Pradesh-500 014, IndiaDepartment of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda Dhulapally, Secunderabad, Andhra Pradesh-500 014, IndiaDepartment of Pharmaceutical Analysis, Nalanda College of Pharmacy, Nalgonda Andhra Pradesh-508 001, IndiaA high performance liquid chromatographic method with mass detection was developed for determination of Lamivudine and Zidovudine in human plasma. Lamivudine and Zidovudine were extracted from an aliquot of human plasma using Solid Phase Extraction technique, and then injected into a liquid chromatograph equipped with a tandem mass spectrometry detector; quantitation was done by peak area ratio method. A weighted (1/Conc2) linear regression was performed to determine the concentration of analyte. This method demonstrates acceptable performance and is suitable for the determination of Lamivudine and Zidovudine in human plasma over the range of 25.291 to 4046.621 ng/mL and 23.357 to 4057.l4lng/mL respectively using Solid Phase Extraction Procedure. As all the values obtained were within the limits as specified, the method has been successfully used to analyse the human plasma containing Lamivudine and Zidovudine with good recoveries and proved to be robust.http://dx.doi.org/10.1155/2012/716963 |
| spellingShingle | C. Parthiban M. Bhagavan Raju M. Sudhakar D. Sathis Kumar Simultaneous Estimation and Validation for Determination of Lamivudine and Zidovudine in Human Plasma By LCMS/MS Method E-Journal of Chemistry |
| title | Simultaneous Estimation and Validation for Determination of Lamivudine and Zidovudine in Human Plasma By LCMS/MS Method |
| title_full | Simultaneous Estimation and Validation for Determination of Lamivudine and Zidovudine in Human Plasma By LCMS/MS Method |
| title_fullStr | Simultaneous Estimation and Validation for Determination of Lamivudine and Zidovudine in Human Plasma By LCMS/MS Method |
| title_full_unstemmed | Simultaneous Estimation and Validation for Determination of Lamivudine and Zidovudine in Human Plasma By LCMS/MS Method |
| title_short | Simultaneous Estimation and Validation for Determination of Lamivudine and Zidovudine in Human Plasma By LCMS/MS Method |
| title_sort | simultaneous estimation and validation for determination of lamivudine and zidovudine in human plasma by lcms ms method |
| url | http://dx.doi.org/10.1155/2012/716963 |
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