Biodegradable Oxygen‐Generating Microneedle Patches for Regenerative Medicine Applications
Upon injury, regenerating skin is metabolically active and requires oxygen for physiological processes related to wound healing. Such processes can be halted in hypoxic conditions common in chronic wounds. Microneedle arrays (MNAs) have been demonstrated to improve therapeutic delivery and wound hea...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-01-01
|
| Series: | Advanced NanoBiomed Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/anbr.202400093 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850085409534509056 |
|---|---|
| author | Lindsay Barnum Mohamadmahdi Samandari Yasir Suhail Steven Toro Ashkan Novin Pejman Ghelich Jacob Quint Farnooosh Saeedinejad Manu Komma Kshitiz Ali Tamayol |
| author_facet | Lindsay Barnum Mohamadmahdi Samandari Yasir Suhail Steven Toro Ashkan Novin Pejman Ghelich Jacob Quint Farnooosh Saeedinejad Manu Komma Kshitiz Ali Tamayol |
| author_sort | Lindsay Barnum |
| collection | DOAJ |
| description | Upon injury, regenerating skin is metabolically active and requires oxygen for physiological processes related to wound healing. Such processes can be halted in hypoxic conditions common in chronic wounds. Microneedle arrays (MNAs) have been demonstrated to improve therapeutic delivery and wound healing. Recently, few studies have explored the use of oxygen‐releasing MNAs; however, they involve complex manufacturing and handling and fail to eliminate cytotoxic byproducts. To address these challenges, biodegradable and mechanically robust gelatin methacryloyl‐based MNAs are developed that can penetrate the tissue and release oxygen upon exposure to interstitial fluid and wound exudates. The oxygen release rate and biocompatibility of the developed MNAs with different compositions are evaluated and optimized. Interestingly, in vitro studies demonstrate that the optimized compositions can release oxygen at therapeutic levels and significantly increase viability of chronically hypoxic cells to match that of normoxic cells. In vivo studies further confirm that the optimized oxygen‐generating MNAs do not cause any harm or impair healing in a murine model of acute skin injury. Additionally, transcriptomic analysis reveals upregulation of key pathways related to fibroblast motility, lipid metabolism, and a marked reduction in inflammatory signaling, all of which contribute to improved wound healing. The developed strategy can introduce new opportunities in elimination of hypoxia and therefore treatment of chronic wounds. |
| format | Article |
| id | doaj-art-504cd12fb72b445aade72255eb227b6f |
| institution | DOAJ |
| issn | 2699-9307 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj-art-504cd12fb72b445aade72255eb227b6f2025-08-20T02:43:43ZengWiley-VCHAdvanced NanoBiomed Research2699-93072025-01-0151n/an/a10.1002/anbr.202400093Biodegradable Oxygen‐Generating Microneedle Patches for Regenerative Medicine ApplicationsLindsay Barnum0Mohamadmahdi Samandari1Yasir Suhail2Steven Toro3Ashkan Novin4Pejman Ghelich5Jacob Quint6Farnooosh Saeedinejad7Manu Komma8Kshitiz9Ali Tamayol10Department of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USADepartment of Biomedical Engineering University of Connecticut Health Center Farmington CT 06030 USAUpon injury, regenerating skin is metabolically active and requires oxygen for physiological processes related to wound healing. Such processes can be halted in hypoxic conditions common in chronic wounds. Microneedle arrays (MNAs) have been demonstrated to improve therapeutic delivery and wound healing. Recently, few studies have explored the use of oxygen‐releasing MNAs; however, they involve complex manufacturing and handling and fail to eliminate cytotoxic byproducts. To address these challenges, biodegradable and mechanically robust gelatin methacryloyl‐based MNAs are developed that can penetrate the tissue and release oxygen upon exposure to interstitial fluid and wound exudates. The oxygen release rate and biocompatibility of the developed MNAs with different compositions are evaluated and optimized. Interestingly, in vitro studies demonstrate that the optimized compositions can release oxygen at therapeutic levels and significantly increase viability of chronically hypoxic cells to match that of normoxic cells. In vivo studies further confirm that the optimized oxygen‐generating MNAs do not cause any harm or impair healing in a murine model of acute skin injury. Additionally, transcriptomic analysis reveals upregulation of key pathways related to fibroblast motility, lipid metabolism, and a marked reduction in inflammatory signaling, all of which contribute to improved wound healing. The developed strategy can introduce new opportunities in elimination of hypoxia and therefore treatment of chronic wounds.https://doi.org/10.1002/anbr.202400093biomaterialsGelMAmicroneedlesoxygen‐generating materialswound healing |
| spellingShingle | Lindsay Barnum Mohamadmahdi Samandari Yasir Suhail Steven Toro Ashkan Novin Pejman Ghelich Jacob Quint Farnooosh Saeedinejad Manu Komma Kshitiz Ali Tamayol Biodegradable Oxygen‐Generating Microneedle Patches for Regenerative Medicine Applications Advanced NanoBiomed Research biomaterials GelMA microneedles oxygen‐generating materials wound healing |
| title | Biodegradable Oxygen‐Generating Microneedle Patches for Regenerative Medicine Applications |
| title_full | Biodegradable Oxygen‐Generating Microneedle Patches for Regenerative Medicine Applications |
| title_fullStr | Biodegradable Oxygen‐Generating Microneedle Patches for Regenerative Medicine Applications |
| title_full_unstemmed | Biodegradable Oxygen‐Generating Microneedle Patches for Regenerative Medicine Applications |
| title_short | Biodegradable Oxygen‐Generating Microneedle Patches for Regenerative Medicine Applications |
| title_sort | biodegradable oxygen generating microneedle patches for regenerative medicine applications |
| topic | biomaterials GelMA microneedles oxygen‐generating materials wound healing |
| url | https://doi.org/10.1002/anbr.202400093 |
| work_keys_str_mv | AT lindsaybarnum biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT mohamadmahdisamandari biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT yasirsuhail biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT steventoro biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT ashkannovin biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT pejmanghelich biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT jacobquint biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT farnoooshsaeedinejad biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT manukomma biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT kshitiz biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications AT alitamayol biodegradableoxygengeneratingmicroneedlepatchesforregenerativemedicineapplications |