Modulating Signal Generation in Aptamer‐Based CNT‐FET Biosensors by Controlling the Functionalization Route
Abstract The identification of biomarkers is key to the early detection of physiological dysfunction. Nanoscale field‐effect transistors (FETs) modified with target‐specific receptors enable direct target sensing, offering enhanced sensitivity due to nanoscale channel confinement. In this regard, si...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-06-01
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| Series: | Advanced Sensor Research |
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| Online Access: | https://doi.org/10.1002/adsr.70002 |
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| author | Haosen Miao Gririraj Manoharan Ahran Lim Peter Mirau Jorge L. Chávez Chang‐Seuk Lee Matteo Palma |
| author_facet | Haosen Miao Gririraj Manoharan Ahran Lim Peter Mirau Jorge L. Chávez Chang‐Seuk Lee Matteo Palma |
| author_sort | Haosen Miao |
| collection | DOAJ |
| description | Abstract The identification of biomarkers is key to the early detection of physiological dysfunction. Nanoscale field‐effect transistors (FETs) modified with target‐specific receptors enable direct target sensing, offering enhanced sensitivity due to nanoscale channel confinement. In this regard, single‐walled carbon nanotubes (SWCNTs) have emerged as strong candidates for the development of transistor‐based biosensors. Understanding the structural parameters that affect sensing performance in such nanoscale electrical detection platforms is essential for their reliable and controllable use. Here, this is investigated that how different assembly strategies employed in the construction of nanoscale aptamer‐based SWCNT‐FET biosensors can dramatically affect their signal generation, with conductance increasing or decreasing for the same aptamer‐cortisol recognition event. a cortisol‐binding DNA aptamer exhibiting well‐characterized conformational behavior is employed, as a model receptor to explore the influence of different surface functionalization strategies on SWCNT‐based biosensors performance. Through combined electrical and optical characterization, this is elucidated that how aptamer conformation governs local electrostatic changes within the Debye length, which in turn modulates the electrostatic gating of the devices. This work offers insight into effective design strategies for the construction of biosensors functionalized with electrostatically active molecular receptors. |
| format | Article |
| id | doaj-art-504bd8cf201e4e07a16c346385c4edf3 |
| institution | OA Journals |
| issn | 2751-1219 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Sensor Research |
| spelling | doaj-art-504bd8cf201e4e07a16c346385c4edf32025-08-20T02:31:20ZengWiley-VCHAdvanced Sensor Research2751-12192025-06-0146n/an/a10.1002/adsr.70002Modulating Signal Generation in Aptamer‐Based CNT‐FET Biosensors by Controlling the Functionalization RouteHaosen Miao0Gririraj Manoharan1Ahran Lim2Peter Mirau3Jorge L. Chávez4Chang‐Seuk Lee5Matteo Palma6Department of Chemistry Queen Mary University of London London E1 4NS UKDepartment of Chemistry Queen Mary University of London London E1 4NS UKDepartment of Chemistry Seoul Women's University Seoul 01797 Republic of KoreaMaterials and Manufacturing Directorate Air Force Research Laboratory Ohio OH 45433 USA711th Human Performance Wing Air Force Research Laboratory Ohio OH 45433 USADepartment of Chemistry Seoul Women's University Seoul 01797 Republic of KoreaDepartment of Chemistry Queen Mary University of London London E1 4NS UKAbstract The identification of biomarkers is key to the early detection of physiological dysfunction. Nanoscale field‐effect transistors (FETs) modified with target‐specific receptors enable direct target sensing, offering enhanced sensitivity due to nanoscale channel confinement. In this regard, single‐walled carbon nanotubes (SWCNTs) have emerged as strong candidates for the development of transistor‐based biosensors. Understanding the structural parameters that affect sensing performance in such nanoscale electrical detection platforms is essential for their reliable and controllable use. Here, this is investigated that how different assembly strategies employed in the construction of nanoscale aptamer‐based SWCNT‐FET biosensors can dramatically affect their signal generation, with conductance increasing or decreasing for the same aptamer‐cortisol recognition event. a cortisol‐binding DNA aptamer exhibiting well‐characterized conformational behavior is employed, as a model receptor to explore the influence of different surface functionalization strategies on SWCNT‐based biosensors performance. Through combined electrical and optical characterization, this is elucidated that how aptamer conformation governs local electrostatic changes within the Debye length, which in turn modulates the electrostatic gating of the devices. This work offers insight into effective design strategies for the construction of biosensors functionalized with electrostatically active molecular receptors.https://doi.org/10.1002/adsr.70002DNA aptamersFET biosensorssingle‐walled carbon nanotubes |
| spellingShingle | Haosen Miao Gririraj Manoharan Ahran Lim Peter Mirau Jorge L. Chávez Chang‐Seuk Lee Matteo Palma Modulating Signal Generation in Aptamer‐Based CNT‐FET Biosensors by Controlling the Functionalization Route Advanced Sensor Research DNA aptamers FET biosensors single‐walled carbon nanotubes |
| title | Modulating Signal Generation in Aptamer‐Based CNT‐FET Biosensors by Controlling the Functionalization Route |
| title_full | Modulating Signal Generation in Aptamer‐Based CNT‐FET Biosensors by Controlling the Functionalization Route |
| title_fullStr | Modulating Signal Generation in Aptamer‐Based CNT‐FET Biosensors by Controlling the Functionalization Route |
| title_full_unstemmed | Modulating Signal Generation in Aptamer‐Based CNT‐FET Biosensors by Controlling the Functionalization Route |
| title_short | Modulating Signal Generation in Aptamer‐Based CNT‐FET Biosensors by Controlling the Functionalization Route |
| title_sort | modulating signal generation in aptamer based cnt fet biosensors by controlling the functionalization route |
| topic | DNA aptamers FET biosensors single‐walled carbon nanotubes |
| url | https://doi.org/10.1002/adsr.70002 |
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