Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria
Sperm cryopreservation provides patients undergoing oncological, surgical, or infertility treatments the opportunity to conceive their own children, using assisted reproductive technologies. However, the freezing-thawing process can negatively influence both the quantity and the quality of spermatoz...
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2025-01-01
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| Series: | Antioxidants |
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| author | Hamza Goujet Ismail Kaarouch Abderrahim Malki Modou Mamoune Mbaye Rosalie Cabry Noureddine Louanjli Taha Rhouda Moncef Benkhalifa |
| author_facet | Hamza Goujet Ismail Kaarouch Abderrahim Malki Modou Mamoune Mbaye Rosalie Cabry Noureddine Louanjli Taha Rhouda Moncef Benkhalifa |
| author_sort | Hamza Goujet |
| collection | DOAJ |
| description | Sperm cryopreservation provides patients undergoing oncological, surgical, or infertility treatments the opportunity to conceive their own children, using assisted reproductive technologies. However, the freezing-thawing process can negatively influence both the quantity and the quality of spermatozoa, mainly due to an excessive production of reactive oxygen species and/or an impaired antioxidant defense system in sperm. Aromatic and medicinal plants synthesize essential oils with antioxidant proprieties as a part of their ecological adaptation to environmental stress, thanks to their rich bioactive phytochemical components. This study aimed to assess sperm progressive motility, viability, plasma membrane functionality, and lipid peroxidation levels of human cryopreserved normozoospermic (n = 51) and asthenozoospermic (n = 51) samples without or with the addition of <i>Thymus satureoides</i> (TSEO) (20 µg/mL), <i>Artemisia vulgaris</i> (AVEO) (48 µg/mL), and <i>Rosmarinus officinalis</i> (ROEO) (13 µg/mL) essential oils. Sperm parameters were significantly better preserved with ROEO in both normozoospermic (<i>p</i> < 0.05) and asthenozoospermic samples (<i>p</i> < 0.01). In contrast, TSEO had a negative impact for both groups (<i>p</i> < 0.05). Meanwhile, no significant effects were observed with AVEO. In summary, the study revealed that in vitro addition of essential oils as antioxidant agents during cryopreservation can be either beneficial, which helps preserve sperm parameters and fertilizing potential, or detrimental as spermicidal agents. |
| format | Article |
| id | doaj-art-50411706038945f88918e7fa7d17489a |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-50411706038945f88918e7fa7d17489a2025-01-24T13:19:24ZengMDPI AGAntioxidants2076-39212025-01-011417510.3390/antiox14010075Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm CriteriaHamza Goujet0Ismail Kaarouch1Abderrahim Malki2Modou Mamoune Mbaye3Rosalie Cabry4Noureddine Louanjli5Taha Rhouda6Moncef Benkhalifa7Laboratory of Physiopathology and Molecular Genetics, Ben M’Sik Faculty of Sciences, Hassan II University of Casablanca, Casablanca 20670, MoroccoAfrican Fertility Center, Private Clinic of Human Reproduction and Endoscopic Surgery, Casablanca 20000, MoroccoLaboratory of Physiopathology and Molecular Genetics, Ben M’Sik Faculty of Sciences, Hassan II University of Casablanca, Casablanca 20670, MoroccoLaboratory of Medical Analyses, Reproductive Biology, LABOMAC, IRIFIV Centre, Casablanca 20100, MoroccoReproductive Medicine, Reproductive Biology and Genetics, Peritox Laboratory, University Hospital and School of Medicine, Picardie University Jules Verne, 80054 Amiens, FranceLaboratory of Medical Analyses, Reproductive Biology, LABOMAC, IRIFIV Centre, Casablanca 20100, MoroccoLaboratory of Physiopathology and Molecular Genetics, Ben M’Sik Faculty of Sciences, Hassan II University of Casablanca, Casablanca 20670, MoroccoReproductive Medicine, Reproductive Biology and Genetics, Peritox Laboratory, University Hospital and School of Medicine, Picardie University Jules Verne, 80054 Amiens, FranceSperm cryopreservation provides patients undergoing oncological, surgical, or infertility treatments the opportunity to conceive their own children, using assisted reproductive technologies. However, the freezing-thawing process can negatively influence both the quantity and the quality of spermatozoa, mainly due to an excessive production of reactive oxygen species and/or an impaired antioxidant defense system in sperm. Aromatic and medicinal plants synthesize essential oils with antioxidant proprieties as a part of their ecological adaptation to environmental stress, thanks to their rich bioactive phytochemical components. This study aimed to assess sperm progressive motility, viability, plasma membrane functionality, and lipid peroxidation levels of human cryopreserved normozoospermic (n = 51) and asthenozoospermic (n = 51) samples without or with the addition of <i>Thymus satureoides</i> (TSEO) (20 µg/mL), <i>Artemisia vulgaris</i> (AVEO) (48 µg/mL), and <i>Rosmarinus officinalis</i> (ROEO) (13 µg/mL) essential oils. Sperm parameters were significantly better preserved with ROEO in both normozoospermic (<i>p</i> < 0.05) and asthenozoospermic samples (<i>p</i> < 0.01). In contrast, TSEO had a negative impact for both groups (<i>p</i> < 0.05). Meanwhile, no significant effects were observed with AVEO. In summary, the study revealed that in vitro addition of essential oils as antioxidant agents during cryopreservation can be either beneficial, which helps preserve sperm parameters and fertilizing potential, or detrimental as spermicidal agents.https://www.mdpi.com/2076-3921/14/1/75sperm cryopreservationessential oilsfertility preservationsperm quality |
| spellingShingle | Hamza Goujet Ismail Kaarouch Abderrahim Malki Modou Mamoune Mbaye Rosalie Cabry Noureddine Louanjli Taha Rhouda Moncef Benkhalifa Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria Antioxidants sperm cryopreservation essential oils fertility preservation sperm quality |
| title | Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria |
| title_full | Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria |
| title_fullStr | Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria |
| title_full_unstemmed | Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria |
| title_short | Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria |
| title_sort | effects of essential oils as antioxidant and cryoprotective agents in improving frozen and thawed human sperm criteria |
| topic | sperm cryopreservation essential oils fertility preservation sperm quality |
| url | https://www.mdpi.com/2076-3921/14/1/75 |
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