The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview
Acute respiratory tract infection is a common public health concern worldwide a new emerging contagious virus (COVID-2019) or SARSC- 2 causing a pandemic pneumonia outbreak, The main transmission route of this virus is through droplets from respiratory made during sneezing or coughing of infected pe...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sulaimani Polytechnic University
2020-04-01
|
| Series: | Kurdistan Journal of Applied Research |
| Subjects: | |
| Online Access: | https://kjar.spu.edu.iq/index.php/kjar/article/view/487 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823861331046957056 |
|---|---|
| author | Abdullah Ahmed Hama Othman Abdulrahman Mohammed Fatima Mahmud Ali Osama Hamid Shareef Sardar Muhammad Wli Sabiha Sharif Syamand Ahmed Qadir |
| author_facet | Abdullah Ahmed Hama Othman Abdulrahman Mohammed Fatima Mahmud Ali Osama Hamid Shareef Sardar Muhammad Wli Sabiha Sharif Syamand Ahmed Qadir |
| author_sort | Abdullah Ahmed Hama |
| collection | DOAJ |
| description | Acute respiratory tract infection is a common public health concern worldwide a new emerging contagious virus (COVID-2019) or SARSC- 2 causing a pandemic pneumonia outbreak, The main transmission route of this virus is through droplets from respiratory made during sneezing or coughing of infected people like the recent viral infection of severe acute respiratory syndrome (SARS-CoV1) and the Middle East respiratory syndrome (MERS). Many epidemiological factors have a crucial role in promoting the transmission of the COVID-2019 that makes the disease as an emerging and global alarming against this new coronavirus. Early diagnosis of the etiological agents is critical for appropriate management, controlling plan, protection, and treatment. The new outbreak of COVID-19 can be detected by different molecular protocols. Quantitative polymerase chain reaction (qPCR) is the recommended technique used with varied sensitivity due to primers variation and specimen type. The reliable, high specific and sensitive diagnosis protocols are necessary for an emerging control plan. This study will review and explore the most available methods of molecular identification and primers for the diagnosis of the new coronavirus (COVID-19). This review will also open the new clues to develop and select appropriate diagnosis panel and specific primers for new coronavirus. In conclusion of this review, the RNA dependent RNA polymerase (RdRp) and RdRp/Hel protocols will be valuable to distinguish the COVID-19 from the SARS-CoV and the other respiratory viral pathogens.
|
| format | Article |
| id | doaj-art-503bd1d3ff284ca8b84a8de050006653 |
| institution | Kabale University |
| issn | 2411-7684 2411-7706 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Sulaimani Polytechnic University |
| record_format | Article |
| series | Kurdistan Journal of Applied Research |
| spelling | doaj-art-503bd1d3ff284ca8b84a8de0500066532025-02-09T21:00:20ZengSulaimani Polytechnic UniversityKurdistan Journal of Applied Research2411-76842411-77062020-04-015310.24017/covid.2The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an OverviewAbdullah Ahmed Hama0Othman Abdulrahman Mohammed1Fatima Mahmud Ali2Osama Hamid Shareef3Sardar Muhammad Wli4Sabiha Sharif5Syamand Ahmed Qadir6Medical Laboratory Department, Technical College of Health | Research Center, Sulaimani Polytechnic University, Sulaimani, IraqMedical Laboratory Department | Research Center, Technical College of Applied Science in Halabja, Sulaimani Polytechnic university Sulaimani, IraqNursing Department, Sulaimani Technical Institute | Research Center, Sulaimani Polytechnic University, Sulaimani, IraqMedical Laboratory Science, Technical College of Applied Science in Halabja | Research Center, Sulaimani Polytechnic University,Sulaimani, IraqNursing department, Technical College of Health | Research Center, Sulaimani Polytechnic University, Sulaimani, IraqMedical Laboratory Department, Technical College of Health | Research Center, Sulaimani Polytechnic university Sulaimani, IraqMedical Laboratory Science, Technical College of Applied Science in Halabja | Research center, Sulaimani Polytechnic University, Sulaimani, IraqAcute respiratory tract infection is a common public health concern worldwide a new emerging contagious virus (COVID-2019) or SARSC- 2 causing a pandemic pneumonia outbreak, The main transmission route of this virus is through droplets from respiratory made during sneezing or coughing of infected people like the recent viral infection of severe acute respiratory syndrome (SARS-CoV1) and the Middle East respiratory syndrome (MERS). Many epidemiological factors have a crucial role in promoting the transmission of the COVID-2019 that makes the disease as an emerging and global alarming against this new coronavirus. Early diagnosis of the etiological agents is critical for appropriate management, controlling plan, protection, and treatment. The new outbreak of COVID-19 can be detected by different molecular protocols. Quantitative polymerase chain reaction (qPCR) is the recommended technique used with varied sensitivity due to primers variation and specimen type. The reliable, high specific and sensitive diagnosis protocols are necessary for an emerging control plan. This study will review and explore the most available methods of molecular identification and primers for the diagnosis of the new coronavirus (COVID-19). This review will also open the new clues to develop and select appropriate diagnosis panel and specific primers for new coronavirus. In conclusion of this review, the RNA dependent RNA polymerase (RdRp) and RdRp/Hel protocols will be valuable to distinguish the COVID-19 from the SARS-CoV and the other respiratory viral pathogens. https://kjar.spu.edu.iq/index.php/kjar/article/view/487COVID-19, new coronavirus, diagnostic methods, DNA marker, respiratory viral infection. |
| spellingShingle | Abdullah Ahmed Hama Othman Abdulrahman Mohammed Fatima Mahmud Ali Osama Hamid Shareef Sardar Muhammad Wli Sabiha Sharif Syamand Ahmed Qadir The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview Kurdistan Journal of Applied Research COVID-19, new coronavirus, diagnostic methods, DNA marker, respiratory viral infection. |
| title | The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview |
| title_full | The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview |
| title_fullStr | The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview |
| title_full_unstemmed | The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview |
| title_short | The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview |
| title_sort | molecular diagnosis protocols of new coronavirus covid 19 specificity and sensitivity an overview |
| topic | COVID-19, new coronavirus, diagnostic methods, DNA marker, respiratory viral infection. |
| url | https://kjar.spu.edu.iq/index.php/kjar/article/view/487 |
| work_keys_str_mv | AT abdullahahmedhama themoleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT othmanabdulrahmanmohammed themoleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT fatimamahmudali themoleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT osamahamidshareef themoleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT sardarmuhammadwli themoleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT sabihasharif themoleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT syamandahmedqadir themoleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT abdullahahmedhama moleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT othmanabdulrahmanmohammed moleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT fatimamahmudali moleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT osamahamidshareef moleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT sardarmuhammadwli moleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT sabihasharif moleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview AT syamandahmedqadir moleculardiagnosisprotocolsofnewcoronaviruscovid19specificityandsensitivityanoverview |