Comparative Study of Angiotensin Convertase Enzyme (ACE2) Gene Expression in Patients with Covid-19 By Molecular Method
Introduction: The Covid 19 illness is triggered by a coronavirus known as SARS CoV-2. This particular virus spreads rapidly likely because of the Spike proteins affinity, to the human ACE2 cell receptor causing harm to organs, like the lungs in type 2 pneumocytes that abundantly express this recept...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-12-01
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| Series: | Journal of Craniomaxillofacial Research |
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| Online Access: | https://jcr.tums.ac.ir/index.php/jcr/article/view/508 |
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| author | Behrooz Farzanegan Naghmeh Bahrami Mohammad Fathi Saba Tahmasebi Mehdi Kazem Pour Dizaji Shadi Shafaghi Farnaz Ahmadi Farnoush Mohammadi Seyedeh Zahra Fotook Kiaei Mona Mohajeri Tehrani Masoume Farhangiyan Abdolreza Mohamadnia |
| author_facet | Behrooz Farzanegan Naghmeh Bahrami Mohammad Fathi Saba Tahmasebi Mehdi Kazem Pour Dizaji Shadi Shafaghi Farnaz Ahmadi Farnoush Mohammadi Seyedeh Zahra Fotook Kiaei Mona Mohajeri Tehrani Masoume Farhangiyan Abdolreza Mohamadnia |
| author_sort | Behrooz Farzanegan |
| collection | DOAJ |
| description |
Introduction: The Covid 19 illness is triggered by a coronavirus known as SARS CoV-2. This particular virus spreads rapidly likely because of the Spike proteins affinity, to the human ACE2 cell receptor causing harm to organs, like the lungs in type 2 pneumocytes that abundantly express this receptor.
Materials and Methods: The research methodology used in this study involved a case control approach. Patients were referred by a specialist following diagnosis. A total of 50 samples, from infectious cases and 50 samples from cases, with mild symptoms were analyzed for statistical purposes. The ACE2 gene expression was assessed using the Realtime RCR method after RNA extraction.
Results: The findings indicate that the average age does not have an impact on individuals experiencing mild symptoms. Moreover, there is a decrease in the expression of ACE2 genes in the blood of patients with severe symptoms hospitalized in intensive care units as compared to those, with mild symptoms, highlighting a substantial disparity.
Conclusion: The numerical value of fold change for ACE2 gene in severe type patients shows a decrease compared to mild patients and it is 2.11 times lower in severe type patients than mild type.
Keywords: COVID-19; ACE2; Realtime RCR.
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| format | Article |
| id | doaj-art-501f02494db742efb3afec47e008b247 |
| institution | Kabale University |
| issn | 2345-5489 2345-6213 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Craniomaxillofacial Research |
| spelling | doaj-art-501f02494db742efb3afec47e008b2472025-01-06T08:41:00ZengTehran University of Medical SciencesJournal of Craniomaxillofacial Research2345-54892345-62132024-12-01113Comparative Study of Angiotensin Convertase Enzyme (ACE2) Gene Expression in Patients with Covid-19 By Molecular MethodBehrooz Farzanegan0Naghmeh Bahrami1Mohammad Fathi2Saba Tahmasebi3Mehdi Kazem Pour Dizaji4Shadi Shafaghi5Farnaz Ahmadi6Farnoush Mohammadi7Seyedeh Zahra Fotook Kiaei8Mona Mohajeri Tehrani9Masoume Farhangiyan10Abdolreza Mohamadnia11Critical Care Quality Improvement Research Center, SBMU, Tehran, Iran.Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.Anesthesiology Research Center, Department of Anesthesia and Critical Care, Shahid Modares Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Anesthesiology Research Center, Department of Anesthesia and Critical Care, Shahid Modares Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Biostatistics, Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Lung Transplantation Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Lung Transplantation Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pulmonary and Critical Care, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran.Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran.Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran, And Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Introduction: The Covid 19 illness is triggered by a coronavirus known as SARS CoV-2. This particular virus spreads rapidly likely because of the Spike proteins affinity, to the human ACE2 cell receptor causing harm to organs, like the lungs in type 2 pneumocytes that abundantly express this receptor. Materials and Methods: The research methodology used in this study involved a case control approach. Patients were referred by a specialist following diagnosis. A total of 50 samples, from infectious cases and 50 samples from cases, with mild symptoms were analyzed for statistical purposes. The ACE2 gene expression was assessed using the Realtime RCR method after RNA extraction. Results: The findings indicate that the average age does not have an impact on individuals experiencing mild symptoms. Moreover, there is a decrease in the expression of ACE2 genes in the blood of patients with severe symptoms hospitalized in intensive care units as compared to those, with mild symptoms, highlighting a substantial disparity. Conclusion: The numerical value of fold change for ACE2 gene in severe type patients shows a decrease compared to mild patients and it is 2.11 times lower in severe type patients than mild type. Keywords: COVID-19; ACE2; Realtime RCR. https://jcr.tums.ac.ir/index.php/jcr/article/view/508COVID-19; ACE2; Realtime RCR. |
| spellingShingle | Behrooz Farzanegan Naghmeh Bahrami Mohammad Fathi Saba Tahmasebi Mehdi Kazem Pour Dizaji Shadi Shafaghi Farnaz Ahmadi Farnoush Mohammadi Seyedeh Zahra Fotook Kiaei Mona Mohajeri Tehrani Masoume Farhangiyan Abdolreza Mohamadnia Comparative Study of Angiotensin Convertase Enzyme (ACE2) Gene Expression in Patients with Covid-19 By Molecular Method Journal of Craniomaxillofacial Research COVID-19; ACE2; Realtime RCR. |
| title | Comparative Study of Angiotensin Convertase Enzyme (ACE2) Gene Expression in Patients with Covid-19 By Molecular Method |
| title_full | Comparative Study of Angiotensin Convertase Enzyme (ACE2) Gene Expression in Patients with Covid-19 By Molecular Method |
| title_fullStr | Comparative Study of Angiotensin Convertase Enzyme (ACE2) Gene Expression in Patients with Covid-19 By Molecular Method |
| title_full_unstemmed | Comparative Study of Angiotensin Convertase Enzyme (ACE2) Gene Expression in Patients with Covid-19 By Molecular Method |
| title_short | Comparative Study of Angiotensin Convertase Enzyme (ACE2) Gene Expression in Patients with Covid-19 By Molecular Method |
| title_sort | comparative study of angiotensin convertase enzyme ace2 gene expression in patients with covid 19 by molecular method |
| topic | COVID-19; ACE2; Realtime RCR. |
| url | https://jcr.tums.ac.ir/index.php/jcr/article/view/508 |
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