HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced Colitis
Inflammatory bowel disease (IBD) is highly prevalent globally and, in the majority of cases, remains asymptomatic during its initial stages. The gastrointestinal microbiota secretes volatile organic compounds (VOCs), and their composition alters in IBD. The examination of VOCs could prove beneficial...
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Bio-protocol LLC
2025-03-01
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| Online Access: | https://bio-protocol.org/en/bpdetail?id=5246&type=0 |
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| author | Olga Shagaleeva Daria Kashatnikova Dmitry Kardonsky Boris Efimov Viktor Ivanov Svetlana Smirnova Yana Zorkina Elizaveta Vorobjeva Artemiy Silantiev Viktoriia Kazakova Irina Kolesnikova Maria Markelova Anna Vanyushkina Andrei Chaplin Tatiana Grigoryeva Natalya Zakharzhevskaya |
| author_facet | Olga Shagaleeva Daria Kashatnikova Dmitry Kardonsky Boris Efimov Viktor Ivanov Svetlana Smirnova Yana Zorkina Elizaveta Vorobjeva Artemiy Silantiev Viktoriia Kazakova Irina Kolesnikova Maria Markelova Anna Vanyushkina Andrei Chaplin Tatiana Grigoryeva Natalya Zakharzhevskaya |
| author_sort | Olga Shagaleeva |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) is highly prevalent globally and, in the majority of cases, remains asymptomatic during its initial stages. The gastrointestinal microbiota secretes volatile organic compounds (VOCs), and their composition alters in IBD. The examination of VOCs could prove beneficial in complementing diagnostic techniques to facilitate the early identification of IBD risk. In this protocol, a model of sodium dextran sulfate (DSS)-induced colitis in rats was successfully implemented for the non-invasive metabolomic assessment of different stages of inflammation. Headspace–gas chromatography–mass spectrometry (HS–GC–MS) was used as a non-invasive method for inflammation assessment at early and remission stages. The disease activity index (DAI) and histological method were employed to assess intestinal inflammation. The HS–GC–MS method demonstrated high sensitivity to intestine inflammation, confirmed by DAI and histology assay, in the acute and remission stages, identifying changes in the relative content of VOCs in stools. HS–GC–MS may be a useful and non-invasive method for IBD diagnostics and therapy effectiveness control. |
| format | Article |
| id | doaj-art-50162dce4cff4ec7bf7fecaeb7e640f6 |
| institution | DOAJ |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-50162dce4cff4ec7bf7fecaeb7e640f62025-08-20T02:41:14ZengBio-protocol LLCBio-Protocol2331-83252025-03-0115610.21769/BioProtoc.5246HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced ColitisOlga Shagaleeva0Daria Kashatnikova1Dmitry Kardonsky2Boris Efimov3Viktor Ivanov4Svetlana Smirnova5Yana Zorkina6Elizaveta Vorobjeva7Artemiy Silantiev8Viktoriia Kazakova9Irina Kolesnikova10Maria Markelova11Anna Vanyushkina12Andrei Chaplin13Tatiana Grigoryeva14Natalya Zakharzhevskaya15Laboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaLaboratory of Ecological Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaDepartment of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaLaboratory of Ecological Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, RussiaDepartment of Basic and Applied Neurobiology, V. P. Serbsky National Medical Research Center for Psychiatry and Narcology, Moscow, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, RussiaVladimir Zelman Center for Neurobiology and Brain Rehabilitation, Skolkovo Institute of Science and Technology, MoscowLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaDepartment of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow, RussiaInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, RussiaLaboratory of Molecular Pathophysiology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaInflammatory bowel disease (IBD) is highly prevalent globally and, in the majority of cases, remains asymptomatic during its initial stages. The gastrointestinal microbiota secretes volatile organic compounds (VOCs), and their composition alters in IBD. The examination of VOCs could prove beneficial in complementing diagnostic techniques to facilitate the early identification of IBD risk. In this protocol, a model of sodium dextran sulfate (DSS)-induced colitis in rats was successfully implemented for the non-invasive metabolomic assessment of different stages of inflammation. Headspace–gas chromatography–mass spectrometry (HS–GC–MS) was used as a non-invasive method for inflammation assessment at early and remission stages. The disease activity index (DAI) and histological method were employed to assess intestinal inflammation. The HS–GC–MS method demonstrated high sensitivity to intestine inflammation, confirmed by DAI and histology assay, in the acute and remission stages, identifying changes in the relative content of VOCs in stools. HS–GC–MS may be a useful and non-invasive method for IBD diagnostics and therapy effectiveness control.https://bio-protocol.org/en/bpdetail?id=5246&type=0 |
| spellingShingle | Olga Shagaleeva Daria Kashatnikova Dmitry Kardonsky Boris Efimov Viktor Ivanov Svetlana Smirnova Yana Zorkina Elizaveta Vorobjeva Artemiy Silantiev Viktoriia Kazakova Irina Kolesnikova Maria Markelova Anna Vanyushkina Andrei Chaplin Tatiana Grigoryeva Natalya Zakharzhevskaya HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced Colitis Bio-Protocol |
| title | HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced Colitis |
| title_full | HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced Colitis |
| title_fullStr | HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced Colitis |
| title_full_unstemmed | HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced Colitis |
| title_short | HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced Colitis |
| title_sort | hs gc ms method for the diagnosis of ibd dynamics in a model of dss induced colitis |
| url | https://bio-protocol.org/en/bpdetail?id=5246&type=0 |
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