Genomic analysis and antibiotic resistance of a multidrug-resistant bacterium isolated from pharmaceutical wastewater treatment plant sludge
Pharmaceutical wastewater treatment plants (PWWTPs) serve as reservoirs for antibiotic-resistant bacteria (ARBs) and antibiotic resistance genes (ARGs). In this study, a multiantibiotic-resistant strain of Acinetobacter lwoffii (named N4) was isolated from the dewatered sludge of a PWWTP. N4 exhibit...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015227 |
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| author | Ningyu Sun Hu Gao Xinbo Zhang Zeyou Chen Anping Peng |
| author_facet | Ningyu Sun Hu Gao Xinbo Zhang Zeyou Chen Anping Peng |
| author_sort | Ningyu Sun |
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| description | Pharmaceutical wastewater treatment plants (PWWTPs) serve as reservoirs for antibiotic-resistant bacteria (ARBs) and antibiotic resistance genes (ARGs). In this study, a multiantibiotic-resistant strain of Acinetobacter lwoffii (named N4) was isolated from the dewatered sludge of a PWWTP. N4 exhibited high resistance to both antibiotics and metals, with minimum inhibitory concentrations (MICs) of chloramphenicol and cefazolin reaching 1024 mg·L−1 and MICs of Cu2+ and Zn2+ reaching 512 mg·L−1. Co-sensitization experiments revealed that when antibiotics are co-existing with heavy metal ions (such as TET and Cd2+, AMP and Cu2+) could enhance the resistance of N4 to them. Whole-genome sequencing of N4 revealed a genome size of 0.37 Mb encoding 3359 genes. Among these, 23 ARGs were identified, including dfrA26, bl2beCTXM, catB3, qnrB, rosB, tlrC, smeD, smeE, mexE, ceoB, oprN, acrB, adeF, ykkC, ksgA and sul2, which confer resistance through mechanisms such as efflux pumps, enzyme modification and target bypass. Additionally, the N4 genome contained 187 genes associated with human disease and 249 virulence factors, underscoring its potential pathogenicity. Overall, this study provides valuable insights into ARBs in PWWTPs and highlights the potential risks posed by multidrug-resistant strains such as N4. |
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| institution | Kabale University |
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| language | English |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-50141dd3def84a7ea0f3ca662ff4b5322025-01-23T05:25:34ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117446Genomic analysis and antibiotic resistance of a multidrug-resistant bacterium isolated from pharmaceutical wastewater treatment plant sludgeNingyu Sun0Hu Gao1Xinbo Zhang2Zeyou Chen3Anping Peng4Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, China; Joint Research Centre for Protective Infrastructure Technology and Environmental Green Bioprocess, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, ChinaTianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, China; Joint Research Centre for Protective Infrastructure Technology and Environmental Green Bioprocess, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, China; Jinan Municipal Engineering Design and Research Institute (Group) Co., Ltd., Xuzhou Branch, Xuzhou, Jiangsu 221000, ChinaTianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, China; Joint Research Centre for Protective Infrastructure Technology and Environmental Green Bioprocess, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, ChinaCollege of Environmental Science and Engineering, Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, Tianjin 300071, ChinaTianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, China; Joint Research Centre for Protective Infrastructure Technology and Environmental Green Bioprocess, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, China; Corresponding author at: Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, NO.26, Jinjing Rd, Xiqing District, Tianjin 300384, China.Pharmaceutical wastewater treatment plants (PWWTPs) serve as reservoirs for antibiotic-resistant bacteria (ARBs) and antibiotic resistance genes (ARGs). In this study, a multiantibiotic-resistant strain of Acinetobacter lwoffii (named N4) was isolated from the dewatered sludge of a PWWTP. N4 exhibited high resistance to both antibiotics and metals, with minimum inhibitory concentrations (MICs) of chloramphenicol and cefazolin reaching 1024 mg·L−1 and MICs of Cu2+ and Zn2+ reaching 512 mg·L−1. Co-sensitization experiments revealed that when antibiotics are co-existing with heavy metal ions (such as TET and Cd2+, AMP and Cu2+) could enhance the resistance of N4 to them. Whole-genome sequencing of N4 revealed a genome size of 0.37 Mb encoding 3359 genes. Among these, 23 ARGs were identified, including dfrA26, bl2beCTXM, catB3, qnrB, rosB, tlrC, smeD, smeE, mexE, ceoB, oprN, acrB, adeF, ykkC, ksgA and sul2, which confer resistance through mechanisms such as efflux pumps, enzyme modification and target bypass. Additionally, the N4 genome contained 187 genes associated with human disease and 249 virulence factors, underscoring its potential pathogenicity. Overall, this study provides valuable insights into ARBs in PWWTPs and highlights the potential risks posed by multidrug-resistant strains such as N4.http://www.sciencedirect.com/science/article/pii/S0147651324015227Acinetobacter lwoffiiPharmaceutical wastewater treatment plants (PWWTPs)Whole-genome sequencing (WGS)Antibiotic resistance genes (ARGs)Resistance Mechanisms |
| spellingShingle | Ningyu Sun Hu Gao Xinbo Zhang Zeyou Chen Anping Peng Genomic analysis and antibiotic resistance of a multidrug-resistant bacterium isolated from pharmaceutical wastewater treatment plant sludge Ecotoxicology and Environmental Safety Acinetobacter lwoffii Pharmaceutical wastewater treatment plants (PWWTPs) Whole-genome sequencing (WGS) Antibiotic resistance genes (ARGs) Resistance Mechanisms |
| title | Genomic analysis and antibiotic resistance of a multidrug-resistant bacterium isolated from pharmaceutical wastewater treatment plant sludge |
| title_full | Genomic analysis and antibiotic resistance of a multidrug-resistant bacterium isolated from pharmaceutical wastewater treatment plant sludge |
| title_fullStr | Genomic analysis and antibiotic resistance of a multidrug-resistant bacterium isolated from pharmaceutical wastewater treatment plant sludge |
| title_full_unstemmed | Genomic analysis and antibiotic resistance of a multidrug-resistant bacterium isolated from pharmaceutical wastewater treatment plant sludge |
| title_short | Genomic analysis and antibiotic resistance of a multidrug-resistant bacterium isolated from pharmaceutical wastewater treatment plant sludge |
| title_sort | genomic analysis and antibiotic resistance of a multidrug resistant bacterium isolated from pharmaceutical wastewater treatment plant sludge |
| topic | Acinetobacter lwoffii Pharmaceutical wastewater treatment plants (PWWTPs) Whole-genome sequencing (WGS) Antibiotic resistance genes (ARGs) Resistance Mechanisms |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015227 |
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