Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules
Nanopipettes, as a class of solid-state nanopores, have evolved into universal tools in biomedicine for the detection of biomarkers and different biological analytes. Nanopipette-based methods combine high sensitivity, selectivity, single-molecule resolution, and multifunctionality. The features hav...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/12/627 |
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| author | Regina M. Kuanaeva Alexander N. Vaneev Petr V. Gorelkin Alexander S. Erofeev |
| author_facet | Regina M. Kuanaeva Alexander N. Vaneev Petr V. Gorelkin Alexander S. Erofeev |
| author_sort | Regina M. Kuanaeva |
| collection | DOAJ |
| description | Nanopipettes, as a class of solid-state nanopores, have evolved into universal tools in biomedicine for the detection of biomarkers and different biological analytes. Nanopipette-based methods combine high sensitivity, selectivity, single-molecule resolution, and multifunctionality. The features have significantly expanded interest in their applications for the biomolecular detection, imaging, and molecular diagnostics of real samples. Moreover, the ease of manufacturing nanopipettes, coupled with their compatibility with fluorescence and electrochemical methods, makes them ideal for portable point-of-care diagnostic devices. This review summarized the latest progress in nanopipette-based nanopore technology for the detection of biomarkers, DNA, RNA, proteins, and peptides, in particular β-amyloid or α-synuclein, emphasizing the impact of technology on molecular diagnostics. By addressing key challenges in single-molecule detection and expanding applications in diverse biological areas, nanopipettes are poised to play a transformative role in the future of personalized medicine. |
| format | Article |
| id | doaj-art-500d8f86a38847d8bd5bd5c982af0dda |
| institution | DOAJ |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-500d8f86a38847d8bd5bd5c982af0dda2025-08-20T02:50:52ZengMDPI AGBiosensors2079-63742024-12-01141262710.3390/bios14120627Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of BiomoleculesRegina M. Kuanaeva0Alexander N. Vaneev1Petr V. Gorelkin2Alexander S. Erofeev3Research Laboratory of Biophysics, National University of Science and Technology “MISIS”, 119049 Moscow, RussiaResearch Laboratory of Biophysics, National University of Science and Technology “MISIS”, 119049 Moscow, RussiaResearch Laboratory of Biophysics, National University of Science and Technology “MISIS”, 119049 Moscow, RussiaResearch Laboratory of Biophysics, National University of Science and Technology “MISIS”, 119049 Moscow, RussiaNanopipettes, as a class of solid-state nanopores, have evolved into universal tools in biomedicine for the detection of biomarkers and different biological analytes. Nanopipette-based methods combine high sensitivity, selectivity, single-molecule resolution, and multifunctionality. The features have significantly expanded interest in their applications for the biomolecular detection, imaging, and molecular diagnostics of real samples. Moreover, the ease of manufacturing nanopipettes, coupled with their compatibility with fluorescence and electrochemical methods, makes them ideal for portable point-of-care diagnostic devices. This review summarized the latest progress in nanopipette-based nanopore technology for the detection of biomarkers, DNA, RNA, proteins, and peptides, in particular β-amyloid or α-synuclein, emphasizing the impact of technology on molecular diagnostics. By addressing key challenges in single-molecule detection and expanding applications in diverse biological areas, nanopipettes are poised to play a transformative role in the future of personalized medicine.https://www.mdpi.com/2079-6374/14/12/627nanopipettenanoporesingle moleculemolecular diagnosticssolid-state nanopore |
| spellingShingle | Regina M. Kuanaeva Alexander N. Vaneev Petr V. Gorelkin Alexander S. Erofeev Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules Biosensors nanopipette nanopore single molecule molecular diagnostics solid-state nanopore |
| title | Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules |
| title_full | Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules |
| title_fullStr | Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules |
| title_full_unstemmed | Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules |
| title_short | Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules |
| title_sort | nanopipettes as a potential diagnostic tool for selective nanopore detection of biomolecules |
| topic | nanopipette nanopore single molecule molecular diagnostics solid-state nanopore |
| url | https://www.mdpi.com/2079-6374/14/12/627 |
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