Sexual Selection in Mosquitofish: Differences in the Use of Mating Cues Between Sexes

Sexual selection is a major driver of speciation and evolution, with mate choice being a key component. Individuals assess mate quality by integrating various mating cues. The Western mosquitofish (<i>Gambusia affinis</i>), a species exhibiting pronounced sexual dimorphism in body size a...

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Main Authors: Jiefei Wei, Bowen Feng, Chenglong Dong, Bojian Chen, Kai Liu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/10/1489
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author Jiefei Wei
Bowen Feng
Chenglong Dong
Bojian Chen
Kai Liu
author_facet Jiefei Wei
Bowen Feng
Chenglong Dong
Bojian Chen
Kai Liu
author_sort Jiefei Wei
collection DOAJ
description Sexual selection is a major driver of speciation and evolution, with mate choice being a key component. Individuals assess mate quality by integrating various mating cues. The Western mosquitofish (<i>Gambusia affinis</i>), a species exhibiting pronounced sexual dimorphism in body size and secondary sexual traits, serves as an ideal model for studying mate choice. This study examines the impact of mating cues on mate choice in different sexes of <i>G. affinis</i> through a combination of morphological parameter database construction, computer-simulated animations, and dichotomous association preference tests. The results showed that male gonopodium status significantly affects female mate choice. Females exhibited a preference for males with resting-phase gonopodia, suggesting their aversion to forced copulation and sexual harassment in coercive mating systems. Furthermore, males preferred younger females, with this preference being positively correlated with male body size. This suggests that males are sensitive to sperm competition intensity and may base their choice on social rank. Geometric morphometric analysis and simulation experiments showed that males preferred females with larger gravid spots, regardless of age, suggesting that gravid spot size reflects female fecundity. Male preference for younger females with streamlined bodies and smaller abdomens was significant, but body size did not affect mate choice in general. Our findings highlight that female and male <i>G. affinis</i> employ different mate choice strategies, with females prioritizing male harassment avoidance and males considering multiple mating cues, not solely one dominant characteristic, in their mate choice decisions. These findings demonstrate that mate choice in <i>G. affinis</i> involves balancing conflicting preferences for traits associated with reduced harassment risk (e.g., resting-phase gonopodium in males) and those linked to reproductive potential (e.g., large gravid spot in females), highlighting the nuanced decision-making processes in both sexes.
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spelling doaj-art-4fffa0519b8a4df5916b484049f9d75d2025-08-20T03:47:52ZengMDPI AGAnimals2076-26152025-05-011510148910.3390/ani15101489Sexual Selection in Mosquitofish: Differences in the Use of Mating Cues Between SexesJiefei Wei0Bowen Feng1Chenglong Dong2Bojian Chen3Kai Liu4Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaDepartment of Biology, Queen’s University, Kingston, ON K7P 3N6, CanadaFisheries College, Jimei University, Xiamen 361021, ChinaSexual selection is a major driver of speciation and evolution, with mate choice being a key component. Individuals assess mate quality by integrating various mating cues. The Western mosquitofish (<i>Gambusia affinis</i>), a species exhibiting pronounced sexual dimorphism in body size and secondary sexual traits, serves as an ideal model for studying mate choice. This study examines the impact of mating cues on mate choice in different sexes of <i>G. affinis</i> through a combination of morphological parameter database construction, computer-simulated animations, and dichotomous association preference tests. The results showed that male gonopodium status significantly affects female mate choice. Females exhibited a preference for males with resting-phase gonopodia, suggesting their aversion to forced copulation and sexual harassment in coercive mating systems. Furthermore, males preferred younger females, with this preference being positively correlated with male body size. This suggests that males are sensitive to sperm competition intensity and may base their choice on social rank. Geometric morphometric analysis and simulation experiments showed that males preferred females with larger gravid spots, regardless of age, suggesting that gravid spot size reflects female fecundity. Male preference for younger females with streamlined bodies and smaller abdomens was significant, but body size did not affect mate choice in general. Our findings highlight that female and male <i>G. affinis</i> employ different mate choice strategies, with females prioritizing male harassment avoidance and males considering multiple mating cues, not solely one dominant characteristic, in their mate choice decisions. These findings demonstrate that mate choice in <i>G. affinis</i> involves balancing conflicting preferences for traits associated with reduced harassment risk (e.g., resting-phase gonopodium in males) and those linked to reproductive potential (e.g., large gravid spot in females), highlighting the nuanced decision-making processes in both sexes.https://www.mdpi.com/2076-2615/15/10/1489mate choicecoercive mating systemmating cues<i>Gambusia affinis</i>
spellingShingle Jiefei Wei
Bowen Feng
Chenglong Dong
Bojian Chen
Kai Liu
Sexual Selection in Mosquitofish: Differences in the Use of Mating Cues Between Sexes
Animals
mate choice
coercive mating system
mating cues
<i>Gambusia affinis</i>
title Sexual Selection in Mosquitofish: Differences in the Use of Mating Cues Between Sexes
title_full Sexual Selection in Mosquitofish: Differences in the Use of Mating Cues Between Sexes
title_fullStr Sexual Selection in Mosquitofish: Differences in the Use of Mating Cues Between Sexes
title_full_unstemmed Sexual Selection in Mosquitofish: Differences in the Use of Mating Cues Between Sexes
title_short Sexual Selection in Mosquitofish: Differences in the Use of Mating Cues Between Sexes
title_sort sexual selection in mosquitofish differences in the use of mating cues between sexes
topic mate choice
coercive mating system
mating cues
<i>Gambusia affinis</i>
url https://www.mdpi.com/2076-2615/15/10/1489
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AT bojianchen sexualselectioninmosquitofishdifferencesintheuseofmatingcuesbetweensexes
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