Study on antifungal active substance of Bacillus subtilis F-2
Effects of Bacillus subtilis F-1 and F-2 on inhibiting the growth of Fusarium oxysporum f. sp. cucumerinum (F. o. c) were studied by a co-culture experiment. The results showed that the growth of F. o. c could be strongly inhibited by the specific metabolites of Bacillus subtilis F-2, but not by Bac...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2007-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.01.0034 |
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| Summary: | Effects of Bacillus subtilis F-1 and F-2 on inhibiting the growth of Fusarium oxysporum f. sp. cucumerinum (F. o. c) were studied by a co-culture experiment. The results showed that the growth of F. o. c could be strongly inhibited by the specific metabolites of Bacillus subtilis F-2, but not by Bacillus subtilis F-1. It was guessed by LC-MS determination that the possible molecular weights of the specific metabolites were 1477.0, 1489.1 and 1505.1. It was further found by MALDI-TOF MS that there were two groups of homologues with a difference of 14 Da in molecular weights in the extract of Bacillus subtilis F-2. In the first group, there were 5 kinds of homologues with the molecular weights of 1462.8, 1476.8, 1490.8, 1504.8 and 1518.9, respectively, which were identified as lipopeptide antibiotics Fengycin B (C<sub>14-18</sub>) by the method of mass fingerprinting. In the second group, the molecular weights of 5 kinds of homologues were 1432.8, 1446.8, 1460.8, 1474.8 and 1488.9, respectively, which were estimated as the isomer of Fengycin A (C<sub>14-18</sub>) as only 2 Da difference in molecular weights. |
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| ISSN: | 1008-9209 2097-5155 |