Infralimbic parvalbumin neural activity facilitates cued threat avoidance

The infralimbic cortex (IL) is essential for flexible behavioral responses to threatening environmental events. Reactive behaviors such as freezing or flight are adaptive in some contexts, but in others a strategic avoidance behavior may be more advantageous. IL has been implicated in avoidance, but...

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Main Authors: Yi-Yun Ho, Qiuwei Yang, Priyanka Boddu, David A Bulkin, Melissa R Warden
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-04-01
Series:eLife
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Online Access:https://elifesciences.org/articles/91221
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author Yi-Yun Ho
Qiuwei Yang
Priyanka Boddu
David A Bulkin
Melissa R Warden
author_facet Yi-Yun Ho
Qiuwei Yang
Priyanka Boddu
David A Bulkin
Melissa R Warden
author_sort Yi-Yun Ho
collection DOAJ
description The infralimbic cortex (IL) is essential for flexible behavioral responses to threatening environmental events. Reactive behaviors such as freezing or flight are adaptive in some contexts, but in others a strategic avoidance behavior may be more advantageous. IL has been implicated in avoidance, but the contribution of distinct IL neural subtypes with differing molecular identities and wiring patterns is poorly understood. Here, we study IL parvalbumin (PV) interneurons in mice as they engage in active avoidance behavior, a behavior in which mice must suppress freezing in order to move to safety. We find that activity in inhibitory PV neurons increases during movement to avoid the shock in this behavioral paradigm, and that PV activity during movement emerges after mice have experienced a single shock, prior to learning avoidance. PV neural activity does not change during movement toward cued rewards or during general locomotion in the open field, behavioral paradigms where freezing does not need to be suppressed to enable movement. Optogenetic suppression of PV neurons increases the duration of freezing and delays the onset of avoidance behavior, but does not affect movement toward rewards or general locomotion. These data provide evidence that IL PV neurons support strategic avoidance behavior by suppressing freezing.
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spelling doaj-art-4fde5daf8aaa45ea825d1448e5cfc1382025-08-20T03:06:53ZengeLife Sciences Publications LtdeLife2050-084X2025-04-011210.7554/eLife.91221Infralimbic parvalbumin neural activity facilitates cued threat avoidanceYi-Yun Ho0https://orcid.org/0000-0002-2465-790XQiuwei Yang1Priyanka Boddu2David A Bulkin3Melissa R Warden4https://orcid.org/0000-0003-2240-3997Department of Neurobiology and Behavior, Cornell University, Ithaca, United States; Cornell Neurotech, Cornell University, Ithaca, United StatesDepartment of Neurobiology and Behavior, Cornell University, Ithaca, United StatesDepartment of Neurobiology and Behavior, Cornell University, Ithaca, United StatesDepartment of Neurobiology and Behavior, Cornell University, Ithaca, United States; Cornell Neurotech, Cornell University, Ithaca, United StatesDepartment of Neurobiology and Behavior, Cornell University, Ithaca, United States; Cornell Neurotech, Cornell University, Ithaca, United States; Department of Translational Neurosciences, University of Arizona College of Medicine, Phoenix, United States; Graduate Interdisciplinary Program in Neuroscience, University of Arizona, Tucson, United StatesThe infralimbic cortex (IL) is essential for flexible behavioral responses to threatening environmental events. Reactive behaviors such as freezing or flight are adaptive in some contexts, but in others a strategic avoidance behavior may be more advantageous. IL has been implicated in avoidance, but the contribution of distinct IL neural subtypes with differing molecular identities and wiring patterns is poorly understood. Here, we study IL parvalbumin (PV) interneurons in mice as they engage in active avoidance behavior, a behavior in which mice must suppress freezing in order to move to safety. We find that activity in inhibitory PV neurons increases during movement to avoid the shock in this behavioral paradigm, and that PV activity during movement emerges after mice have experienced a single shock, prior to learning avoidance. PV neural activity does not change during movement toward cued rewards or during general locomotion in the open field, behavioral paradigms where freezing does not need to be suppressed to enable movement. Optogenetic suppression of PV neurons increases the duration of freezing and delays the onset of avoidance behavior, but does not affect movement toward rewards or general locomotion. These data provide evidence that IL PV neurons support strategic avoidance behavior by suppressing freezing.https://elifesciences.org/articles/91221parvalbuminavoidanceinfralimbicprefrontal corteximagingoptogenetic
spellingShingle Yi-Yun Ho
Qiuwei Yang
Priyanka Boddu
David A Bulkin
Melissa R Warden
Infralimbic parvalbumin neural activity facilitates cued threat avoidance
eLife
parvalbumin
avoidance
infralimbic
prefrontal cortex
imaging
optogenetic
title Infralimbic parvalbumin neural activity facilitates cued threat avoidance
title_full Infralimbic parvalbumin neural activity facilitates cued threat avoidance
title_fullStr Infralimbic parvalbumin neural activity facilitates cued threat avoidance
title_full_unstemmed Infralimbic parvalbumin neural activity facilitates cued threat avoidance
title_short Infralimbic parvalbumin neural activity facilitates cued threat avoidance
title_sort infralimbic parvalbumin neural activity facilitates cued threat avoidance
topic parvalbumin
avoidance
infralimbic
prefrontal cortex
imaging
optogenetic
url https://elifesciences.org/articles/91221
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AT qiuweiyang infralimbicparvalbuminneuralactivityfacilitatescuedthreatavoidance
AT priyankaboddu infralimbicparvalbuminneuralactivityfacilitatescuedthreatavoidance
AT davidabulkin infralimbicparvalbuminneuralactivityfacilitatescuedthreatavoidance
AT melissarwarden infralimbicparvalbuminneuralactivityfacilitatescuedthreatavoidance