Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in Rats
Objective: To evaluate the protective effect and possible mechanism of dandelion jujube juice on D-galactose-induced liver injury in Sprague Dawley (SD) rats. Methods: D-galactose was intraperitoneally injected into the rats at a dose of 500 mg/kg for six weeks to establish a chronic liver injury mo...
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The editorial department of Science and Technology of Food Industry
2024-11-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010068 |
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| author | Jiawei YOU Ruyi HAN Yuexiao CHEN Fen CHEN Yuan LIU Mingyue LI Hongying LI Yiyang LI Yafeng SUN Su TIAN |
| author_facet | Jiawei YOU Ruyi HAN Yuexiao CHEN Fen CHEN Yuan LIU Mingyue LI Hongying LI Yiyang LI Yafeng SUN Su TIAN |
| author_sort | Jiawei YOU |
| collection | DOAJ |
| description | Objective: To evaluate the protective effect and possible mechanism of dandelion jujube juice on D-galactose-induced liver injury in Sprague Dawley (SD) rats. Methods: D-galactose was intraperitoneally injected into the rats at a dose of 500 mg/kg for six weeks to establish a chronic liver injury model. These rats treated with D-galactose were then randomly divided into model group and dandelion jujube juice group (treated with 10 mL/kg·d of dandelion jujube juice by intragastric administration for 28 d). The liver index was calculated, and the pathological changes in liver tissue were observed using HE staining and oil red O staining. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum, malondialdehyde (MDA) content and catalase (CAT) activity in liver tissue were determined by corresponding assay kit. Western blot analysis was used to detect the protein expression, including Kelch like epichlorohydrin associated protein 1 (Keap1), nuclear factor E2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1), BCL2 associated X (Bax), and B-cell lysoma-2 (Bcl-2) proteins in liver tissue. Results: Compared with the blank group, the liver index and liver lipid deposition area were increased in the model group. In addition, the model group showed a significant increase in the levels of serum ALT (P<0.05), AST (P<0.01), liver tissue MDA (P<0.01), Keap1 (P<0.01), Bax (P<0.01) protein expression, as well as a significant decrease in liver tissue CAT activity (P<0.01), Nrf2 (P<0.01), HO-1 (P<0.05), and Bcl-2 (P<0.01) protein expression levels, these indicate liver damage. Furthermore, in comparison with the model group, dandelion jujube juice could significantly reduce liver index (P<0.05), the levels of serum ALT (P<0.05) and AST (P<0.05), liver tissue MDA (P<0.05), Keap1 (P<0.01) protein expression, while CAT activity (P<0.05), Nrf2 (P<0.05), HO-1 (P<0.05) and Bcl-2 (P<0.01) expression in liver tissue were significantly increased. Dandelion jujube juice also significantly improved the morphology of liver tissue and reduced the number of liver cell apoptosis and the area of liver lipid deposition. Conclusion: Dandelion jujube juice may have a certain protective effect against D-galactose-induced liver injury, which may be related to activating the Keap1/Nrf2/HO-1 signaling pathway, improving liver antioxidant capacity, and inhibiting liver cell apoptosis. |
| format | Article |
| id | doaj-art-4fd84fbe2eb144398d364dd7da2fa113 |
| institution | OA Journals |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2024-11-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-4fd84fbe2eb144398d364dd7da2fa1132025-08-20T02:32:38ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-11-01452233133710.13386/j.issn1002-0306.20240100682024010068-22Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in RatsJiawei YOU0Ruyi HAN1Yuexiao CHEN2Fen CHEN3Yuan LIU4Mingyue LI5Hongying LI6Yiyang LI7Yafeng SUN8Su TIAN9Department of Clinical Nutrition, The First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, ChinaTongzhou Center for Disease Control and Prevention, Beijing 101100, ChinaInstitute of Biotechnology and Health, Beijing Academy of Science and Technology, Beijing 100089, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaObjective: To evaluate the protective effect and possible mechanism of dandelion jujube juice on D-galactose-induced liver injury in Sprague Dawley (SD) rats. Methods: D-galactose was intraperitoneally injected into the rats at a dose of 500 mg/kg for six weeks to establish a chronic liver injury model. These rats treated with D-galactose were then randomly divided into model group and dandelion jujube juice group (treated with 10 mL/kg·d of dandelion jujube juice by intragastric administration for 28 d). The liver index was calculated, and the pathological changes in liver tissue were observed using HE staining and oil red O staining. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum, malondialdehyde (MDA) content and catalase (CAT) activity in liver tissue were determined by corresponding assay kit. Western blot analysis was used to detect the protein expression, including Kelch like epichlorohydrin associated protein 1 (Keap1), nuclear factor E2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1), BCL2 associated X (Bax), and B-cell lysoma-2 (Bcl-2) proteins in liver tissue. Results: Compared with the blank group, the liver index and liver lipid deposition area were increased in the model group. In addition, the model group showed a significant increase in the levels of serum ALT (P<0.05), AST (P<0.01), liver tissue MDA (P<0.01), Keap1 (P<0.01), Bax (P<0.01) protein expression, as well as a significant decrease in liver tissue CAT activity (P<0.01), Nrf2 (P<0.01), HO-1 (P<0.05), and Bcl-2 (P<0.01) protein expression levels, these indicate liver damage. Furthermore, in comparison with the model group, dandelion jujube juice could significantly reduce liver index (P<0.05), the levels of serum ALT (P<0.05) and AST (P<0.05), liver tissue MDA (P<0.05), Keap1 (P<0.01) protein expression, while CAT activity (P<0.05), Nrf2 (P<0.05), HO-1 (P<0.05) and Bcl-2 (P<0.01) expression in liver tissue were significantly increased. Dandelion jujube juice also significantly improved the morphology of liver tissue and reduced the number of liver cell apoptosis and the area of liver lipid deposition. Conclusion: Dandelion jujube juice may have a certain protective effect against D-galactose-induced liver injury, which may be related to activating the Keap1/Nrf2/HO-1 signaling pathway, improving liver antioxidant capacity, and inhibiting liver cell apoptosis.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010068dandelion jujube juiced-galactoseliver injuryapoptosisantioxidant |
| spellingShingle | Jiawei YOU Ruyi HAN Yuexiao CHEN Fen CHEN Yuan LIU Mingyue LI Hongying LI Yiyang LI Yafeng SUN Su TIAN Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in Rats Shipin gongye ke-ji dandelion jujube juice d-galactose liver injury apoptosis antioxidant |
| title | Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in Rats |
| title_full | Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in Rats |
| title_fullStr | Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in Rats |
| title_full_unstemmed | Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in Rats |
| title_short | Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in Rats |
| title_sort | protective effect of dandelion jujube juice on d galactose induced liver injury in rats |
| topic | dandelion jujube juice d-galactose liver injury apoptosis antioxidant |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010068 |
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