Use of Fermented Black Tea (<i>Camellia sinensis</i>) Factory Wastes in Standard Rat Diets

Although there are studies on the use of different tea extracts in animal diets, there are no studies on the use of fermented black tea factory production waste (FTFW) in rodent diets. This study aims to evaluate the effects of FTFW, considered an environmentally hazardous waste, in standard rat die...

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Main Authors: Buğra Genç, Nilüfer Kuruca, Gül Fatma Yarım, Tolga Güvenç, Emre Özan, Bahadır Müftüoğlu, Tayfun İde, Aşkın Nur Derinöz Erdoğan, Serdar Odacı
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Veterinary Sciences
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Online Access:https://www.mdpi.com/2306-7381/12/5/451
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author Buğra Genç
Nilüfer Kuruca
Gül Fatma Yarım
Tolga Güvenç
Emre Özan
Bahadır Müftüoğlu
Tayfun İde
Aşkın Nur Derinöz Erdoğan
Serdar Odacı
author_facet Buğra Genç
Nilüfer Kuruca
Gül Fatma Yarım
Tolga Güvenç
Emre Özan
Bahadır Müftüoğlu
Tayfun İde
Aşkın Nur Derinöz Erdoğan
Serdar Odacı
author_sort Buğra Genç
collection DOAJ
description Although there are studies on the use of different tea extracts in animal diets, there are no studies on the use of fermented black tea factory production waste (FTFW) in rodent diets. This study aims to evaluate the effects of FTFW, considered an environmentally hazardous waste, in standard rat diets regarding nutritional, histopathological, and biochemical parameters. In this study, 40 male Wistar albino rats (70 days old, with a live weight of 200–250 g) were divided into four groups, each containing 10 rats. They were fed standard rat diets for 63 days, with varying amounts (0%, 3%, 5%, and 10%) of tea production waste. At the end of the experiment, biochemical analysis of blood and histopathological analysis of all organs were performed. There was no significant difference between the groups in terms of body weight, internal organ weights, or serum biochemical parameters (<i>p</i> > 0.05). No pathological findings were observed in any of the groups. The group receiving 10% tea waste, which had the highest total polyphenol level (1.42 ppm), also showed the highest serum total antioxidant status (TAS) (<i>p</i> < 0.05). In contrast, this group had the lowest total oxidant status (TOS) and oxidative stress index (OSI) levels (<i>p</i> < 0.05). The study concluded that FTFW can be included in the pellet diet of rats without changing their general health status and that a potential environmental pollutant can be used in rat diets.
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issn 2306-7381
language English
publishDate 2025-05-01
publisher MDPI AG
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series Veterinary Sciences
spelling doaj-art-4fd5f16286dd4f968ba5834a7c12b4e22025-08-20T03:12:15ZengMDPI AGVeterinary Sciences2306-73812025-05-0112545110.3390/vetsci12050451Use of Fermented Black Tea (<i>Camellia sinensis</i>) Factory Wastes in Standard Rat DietsBuğra Genç0Nilüfer Kuruca1Gül Fatma Yarım2Tolga Güvenç3Emre Özan4Bahadır Müftüoğlu5Tayfun İde6Aşkın Nur Derinöz Erdoğan7Serdar Odacı8Department of Laboratory Animals, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55100 Samsun, TürkiyeDepartment of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55100 Samsun, TürkiyeDepartment of Biochemistry, Faculty of Veterinary Medicine, Ondokuz Mayis University Samsun, 55100 Samsun, TürkiyeDepartment of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55100 Samsun, TürkiyeDepartment of Laboratory Animals, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55100 Samsun, TürkiyeDepartment of Laboratory Animals, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55100 Samsun, TürkiyeARDEN Research an Experiment, Ankara 06170, TürkiyeARDEN Research an Experiment, Ankara 06170, TürkiyeSamsun Veterinary Control Institute, Republic of Türkiye Ministry of Agriculture and Forestry, Samsun 55200, TürkiyeAlthough there are studies on the use of different tea extracts in animal diets, there are no studies on the use of fermented black tea factory production waste (FTFW) in rodent diets. This study aims to evaluate the effects of FTFW, considered an environmentally hazardous waste, in standard rat diets regarding nutritional, histopathological, and biochemical parameters. In this study, 40 male Wistar albino rats (70 days old, with a live weight of 200–250 g) were divided into four groups, each containing 10 rats. They were fed standard rat diets for 63 days, with varying amounts (0%, 3%, 5%, and 10%) of tea production waste. At the end of the experiment, biochemical analysis of blood and histopathological analysis of all organs were performed. There was no significant difference between the groups in terms of body weight, internal organ weights, or serum biochemical parameters (<i>p</i> > 0.05). No pathological findings were observed in any of the groups. The group receiving 10% tea waste, which had the highest total polyphenol level (1.42 ppm), also showed the highest serum total antioxidant status (TAS) (<i>p</i> < 0.05). In contrast, this group had the lowest total oxidant status (TOS) and oxidative stress index (OSI) levels (<i>p</i> < 0.05). The study concluded that FTFW can be included in the pellet diet of rats without changing their general health status and that a potential environmental pollutant can be used in rat diets.https://www.mdpi.com/2306-7381/12/5/451antioxidantdietfermentationrattea factory waste
spellingShingle Buğra Genç
Nilüfer Kuruca
Gül Fatma Yarım
Tolga Güvenç
Emre Özan
Bahadır Müftüoğlu
Tayfun İde
Aşkın Nur Derinöz Erdoğan
Serdar Odacı
Use of Fermented Black Tea (<i>Camellia sinensis</i>) Factory Wastes in Standard Rat Diets
Veterinary Sciences
antioxidant
diet
fermentation
rat
tea factory waste
title Use of Fermented Black Tea (<i>Camellia sinensis</i>) Factory Wastes in Standard Rat Diets
title_full Use of Fermented Black Tea (<i>Camellia sinensis</i>) Factory Wastes in Standard Rat Diets
title_fullStr Use of Fermented Black Tea (<i>Camellia sinensis</i>) Factory Wastes in Standard Rat Diets
title_full_unstemmed Use of Fermented Black Tea (<i>Camellia sinensis</i>) Factory Wastes in Standard Rat Diets
title_short Use of Fermented Black Tea (<i>Camellia sinensis</i>) Factory Wastes in Standard Rat Diets
title_sort use of fermented black tea i camellia sinensis i factory wastes in standard rat diets
topic antioxidant
diet
fermentation
rat
tea factory waste
url https://www.mdpi.com/2306-7381/12/5/451
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