Association of Immune Cell Phenotypes With Oral Cancer: A Two-Sample Mendelian Randomisation Study

Objectives: The purpose of this study is to assess the potential causal relationship between immune cell phenotype and oral cancer using Mendelian randomisation analysis. Methods: A two-sample Mendelian randomisation (MR) analysis using summary statistics from genome-wide association studies in Euro...

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Main Authors: Yanran Yang, Jiamin Xu, Yanzhu Lu, Zhenxing Tang, Jiajun He
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:International Dental Journal
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Online Access:http://www.sciencedirect.com/science/article/pii/S002065392500084X
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author Yanran Yang
Jiamin Xu
Yanzhu Lu
Zhenxing Tang
Jiajun He
author_facet Yanran Yang
Jiamin Xu
Yanzhu Lu
Zhenxing Tang
Jiajun He
author_sort Yanran Yang
collection DOAJ
description Objectives: The purpose of this study is to assess the potential causal relationship between immune cell phenotype and oral cancer using Mendelian randomisation analysis. Methods: A two-sample Mendelian randomisation (MR) analysis using summary statistics from genome-wide association studies in European populations was conducted to explore causal relationships between immune cell phenotypes and the risk of oral cancer. Inverse-variance weighting, MR-EGGER, simple mode, weighted median, and weighted mode were applied for MR analysis. Sensitivity analyses, including the Steiger test, Cochran's Q test, Egger intercept, and leave-one-out analysis, were performed to assess the robustness of the results. Additionally, colocalisation analysis was carried out to further validate causal associations. Results: A total of 21 immune cell phenotypes were identified as risk factors for oral cancer, while 6 immune cell phenotypes demonstrated protective effects. Sensitivity analyses indicated a lack of robustness in four causal relationships. Genetic variants at rs9469077 on chr6 might be shared between CD28–CD127–CD25++CD8br AC of regulatory T cells and oral cancer. Conclusion: This MR study provides evidence for a strong association between immune cells and oral cancer, highlighting specific immune cell phenotypes as significant risk factors for the development of oral cancer. These findings offer a foundation for future precision immunotherapy strategies for oral cancer. Further studies are required to confirm the relationship between immune cells and oral cancer risk and to elucidate the underlying mechanisms. Clinical Relevance: This study confirms the potential relationship between specific immune cell phenotypes and oral cancer, providing theoretical support for future immunotherapy against oral cancer.
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spelling doaj-art-4fd12a0c551c4fdb876c04b9d6dd3e002025-08-20T01:49:07ZengElsevierInternational Dental Journal0020-65392025-06-017531808181710.1016/j.identj.2025.03.013Association of Immune Cell Phenotypes With Oral Cancer: A Two-Sample Mendelian Randomisation StudyYanran Yang0Jiamin Xu1Yanzhu Lu2Zhenxing Tang3Jiajun He4Corresponding author. Department of Stomatology, Chengdu Seventh People's Hospital, No. 1188, Shuangxing Avenue, Shuangliu District, Chengdu, China.; Department of Stomatology, Chengdu Seventh People's Hospital (Affiliated Cancer Hospital of Chengdu Medical College), Chengdu, ChinaDepartment of Stomatology, Chengdu Seventh People's Hospital (Affiliated Cancer Hospital of Chengdu Medical College), Chengdu, ChinaDepartment of Stomatology, Chengdu Seventh People's Hospital (Affiliated Cancer Hospital of Chengdu Medical College), Chengdu, ChinaDepartment of Stomatology, Chengdu Seventh People's Hospital (Affiliated Cancer Hospital of Chengdu Medical College), Chengdu, ChinaDepartment of Stomatology, Chengdu Seventh People's Hospital (Affiliated Cancer Hospital of Chengdu Medical College), Chengdu, ChinaObjectives: The purpose of this study is to assess the potential causal relationship between immune cell phenotype and oral cancer using Mendelian randomisation analysis. Methods: A two-sample Mendelian randomisation (MR) analysis using summary statistics from genome-wide association studies in European populations was conducted to explore causal relationships between immune cell phenotypes and the risk of oral cancer. Inverse-variance weighting, MR-EGGER, simple mode, weighted median, and weighted mode were applied for MR analysis. Sensitivity analyses, including the Steiger test, Cochran's Q test, Egger intercept, and leave-one-out analysis, were performed to assess the robustness of the results. Additionally, colocalisation analysis was carried out to further validate causal associations. Results: A total of 21 immune cell phenotypes were identified as risk factors for oral cancer, while 6 immune cell phenotypes demonstrated protective effects. Sensitivity analyses indicated a lack of robustness in four causal relationships. Genetic variants at rs9469077 on chr6 might be shared between CD28–CD127–CD25++CD8br AC of regulatory T cells and oral cancer. Conclusion: This MR study provides evidence for a strong association between immune cells and oral cancer, highlighting specific immune cell phenotypes as significant risk factors for the development of oral cancer. These findings offer a foundation for future precision immunotherapy strategies for oral cancer. Further studies are required to confirm the relationship between immune cells and oral cancer risk and to elucidate the underlying mechanisms. Clinical Relevance: This study confirms the potential relationship between specific immune cell phenotypes and oral cancer, providing theoretical support for future immunotherapy against oral cancer.http://www.sciencedirect.com/science/article/pii/S002065392500084XGWASImmune cell phenotypeoralMendelian randomisationOral cancer
spellingShingle Yanran Yang
Jiamin Xu
Yanzhu Lu
Zhenxing Tang
Jiajun He
Association of Immune Cell Phenotypes With Oral Cancer: A Two-Sample Mendelian Randomisation Study
International Dental Journal
GWAS
Immune cell phenotypeoral
Mendelian randomisation
Oral cancer
title Association of Immune Cell Phenotypes With Oral Cancer: A Two-Sample Mendelian Randomisation Study
title_full Association of Immune Cell Phenotypes With Oral Cancer: A Two-Sample Mendelian Randomisation Study
title_fullStr Association of Immune Cell Phenotypes With Oral Cancer: A Two-Sample Mendelian Randomisation Study
title_full_unstemmed Association of Immune Cell Phenotypes With Oral Cancer: A Two-Sample Mendelian Randomisation Study
title_short Association of Immune Cell Phenotypes With Oral Cancer: A Two-Sample Mendelian Randomisation Study
title_sort association of immune cell phenotypes with oral cancer a two sample mendelian randomisation study
topic GWAS
Immune cell phenotypeoral
Mendelian randomisation
Oral cancer
url http://www.sciencedirect.com/science/article/pii/S002065392500084X
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AT jiaminxu associationofimmunecellphenotypeswithoralcanceratwosamplemendelianrandomisationstudy
AT yanzhulu associationofimmunecellphenotypeswithoralcanceratwosamplemendelianrandomisationstudy
AT zhenxingtang associationofimmunecellphenotypeswithoralcanceratwosamplemendelianrandomisationstudy
AT jiajunhe associationofimmunecellphenotypeswithoralcanceratwosamplemendelianrandomisationstudy