Egg Microinjection for the Ladybird Beetle Harmonia axyridis
In this paper, we present a detailed protocol for microinjecting DNA, RNA, or protein solutions into fertilized eggs of the multicolored Asian ladybird beetle, Harmonia axyridis, under a stereomicroscope equipped with an injection apparatus. H. axyridis is an emerging model organism for studying var...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2024-12-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5136&type=0 |
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| author | Taro Nakamura Yuji Matsuoka Teruyuki Niimi |
| author_facet | Taro Nakamura Yuji Matsuoka Teruyuki Niimi |
| author_sort | Taro Nakamura |
| collection | DOAJ |
| description | In this paper, we present a detailed protocol for microinjecting DNA, RNA, or protein solutions into fertilized eggs of the multicolored Asian ladybird beetle, Harmonia axyridis, under a stereomicroscope equipped with an injection apparatus. H. axyridis is an emerging model organism for studying various biological fields, showing intraspecific polymorphisms exhibiting highly diverse color patterns on the elytra. Here, we describe how to rear ladybird beetles in a laboratory and obtain fertilized eggs for microinjection experiments. We also provide a constant fluid flow injection method, which enhances the efficiency of microinjection and improves throughput. Our step-by-step protocol is applicable to generating transgenic or genome-edited ladybird beetles, facilitating functional genetics in H. axyridis; the microinjection method should be applicable to other insect eggs. |
| format | Article |
| id | doaj-art-4fd06d1c4f08416b872a8b5e324796e8 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-4fd06d1c4f08416b872a8b5e324796e82025-02-07T08:16:22ZengBio-protocol LLCBio-Protocol2331-83252024-12-01142410.21769/BioProtoc.5136Egg Microinjection for the Ladybird Beetle Harmonia axyridisTaro Nakamura0Yuji Matsuoka1Teruyuki Niimi2Division of Evolutionary Developmental Biology, National Institute for Basic Biology, Okazaki, JapanBasic Biology Program, Graduate Institute for Advanced Studies, The Graduate University for Advanced Studies, SOKENDAI, Okazaki, JapanDivision of Evolutionary Developmental Biology, National Institute for Basic Biology, Okazaki, JapanDivision of Evolutionary Developmental Biology, National Institute for Basic Biology, Okazaki, JapanBasic Biology Program, Graduate Institute for Advanced Studies, The Graduate University for Advanced Studies, SOKENDAI, Okazaki, JapanIn this paper, we present a detailed protocol for microinjecting DNA, RNA, or protein solutions into fertilized eggs of the multicolored Asian ladybird beetle, Harmonia axyridis, under a stereomicroscope equipped with an injection apparatus. H. axyridis is an emerging model organism for studying various biological fields, showing intraspecific polymorphisms exhibiting highly diverse color patterns on the elytra. Here, we describe how to rear ladybird beetles in a laboratory and obtain fertilized eggs for microinjection experiments. We also provide a constant fluid flow injection method, which enhances the efficiency of microinjection and improves throughput. Our step-by-step protocol is applicable to generating transgenic or genome-edited ladybird beetles, facilitating functional genetics in H. axyridis; the microinjection method should be applicable to other insect eggs.https://bio-protocol.org/en/bpdetail?id=5136&type=0 |
| spellingShingle | Taro Nakamura Yuji Matsuoka Teruyuki Niimi Egg Microinjection for the Ladybird Beetle Harmonia axyridis Bio-Protocol |
| title | Egg Microinjection for the Ladybird Beetle Harmonia axyridis |
| title_full | Egg Microinjection for the Ladybird Beetle Harmonia axyridis |
| title_fullStr | Egg Microinjection for the Ladybird Beetle Harmonia axyridis |
| title_full_unstemmed | Egg Microinjection for the Ladybird Beetle Harmonia axyridis |
| title_short | Egg Microinjection for the Ladybird Beetle Harmonia axyridis |
| title_sort | egg microinjection for the ladybird beetle harmonia axyridis |
| url | https://bio-protocol.org/en/bpdetail?id=5136&type=0 |
| work_keys_str_mv | AT taronakamura eggmicroinjectionfortheladybirdbeetleharmoniaaxyridis AT yujimatsuoka eggmicroinjectionfortheladybirdbeetleharmoniaaxyridis AT teruyukiniimi eggmicroinjectionfortheladybirdbeetleharmoniaaxyridis |