Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS
The aim of this study was to investigate the stability of three major antioxidants of Nigella sativa: thymoquinone (TQ), carvacrol (CR) and thymol (THY), under different stress conditions using HPLC and LC-MS/MS. Forced degradation for each compound was performed under different conditions, includin...
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| Format: | Article |
| Language: | English |
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Sciendo
2020-09-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2020-0028 |
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| author | Soliman Rabab M. Abdel Salam Randa A. Eid Basma G. Khayyat Ahdab Neamatallah Thikryat Mesbah Mostafa K. Hadad Ghada M. |
| author_facet | Soliman Rabab M. Abdel Salam Randa A. Eid Basma G. Khayyat Ahdab Neamatallah Thikryat Mesbah Mostafa K. Hadad Ghada M. |
| author_sort | Soliman Rabab M. |
| collection | DOAJ |
| description | The aim of this study was to investigate the stability of three major antioxidants of Nigella sativa: thymoquinone (TQ), carvacrol (CR) and thymol (THY), under different stress conditions using HPLC and LC-MS/MS. Forced degradation for each compound was performed under different conditions, including oxidation, hydrolysis, photolysis and thermal decomposition. The results showed that both CR and THY were stable under the studied conditions, whereas TQ was not affected by acidic, basic and oxidative forced conditions but the effect of light and heat was significant. The degradation products of TQ were further investigated and characterized by LC-MS/MS. HPLC-UV method has been fully validated in terms of linearity and range, the limit of detection and quantitation, precision, selectivity, accuracy and robustness. The method was successfully applied to quantitative analysis of the principal antioxidants of Nigella sativa TQ, CR and THY in different phytopharmaceuticals. |
| format | Article |
| id | doaj-art-4fd028728eda4d71b78297eb3edbc662 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2020-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-4fd028728eda4d71b78297eb3edbc6622025-02-02T17:46:44ZengSciendoActa Pharmaceutica1846-95582020-09-0170332534210.2478/acph-2020-0028acph-2020-0028Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MSSoliman Rabab M.0Abdel Salam Randa A.1Eid Basma G.2Khayyat Ahdab3Neamatallah Thikryat4Mesbah Mostafa K.5Hadad Ghada M.6Department of Pharmaceutical Chemistry Faculty of Pharmacy, Sinai University El Arish, North Sinai, EgyptDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi ArabiaPharmaceutical Chemistry Department Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, IsmailiaEgyptDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, EgyptThe aim of this study was to investigate the stability of three major antioxidants of Nigella sativa: thymoquinone (TQ), carvacrol (CR) and thymol (THY), under different stress conditions using HPLC and LC-MS/MS. Forced degradation for each compound was performed under different conditions, including oxidation, hydrolysis, photolysis and thermal decomposition. The results showed that both CR and THY were stable under the studied conditions, whereas TQ was not affected by acidic, basic and oxidative forced conditions but the effect of light and heat was significant. The degradation products of TQ were further investigated and characterized by LC-MS/MS. HPLC-UV method has been fully validated in terms of linearity and range, the limit of detection and quantitation, precision, selectivity, accuracy and robustness. The method was successfully applied to quantitative analysis of the principal antioxidants of Nigella sativa TQ, CR and THY in different phytopharmaceuticals.https://doi.org/10.2478/acph-2020-0028thymoquinonecarvacrolthymolhplc-uvlc-ms/msstability |
| spellingShingle | Soliman Rabab M. Abdel Salam Randa A. Eid Basma G. Khayyat Ahdab Neamatallah Thikryat Mesbah Mostafa K. Hadad Ghada M. Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS Acta Pharmaceutica thymoquinone carvacrol thymol hplc-uv lc-ms/ms stability |
| title | Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS |
| title_full | Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS |
| title_fullStr | Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS |
| title_full_unstemmed | Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS |
| title_short | Stability study of thymoquinone, carvacrol and thymol using HPLC-UV and LC-ESI-MS |
| title_sort | stability study of thymoquinone carvacrol and thymol using hplc uv and lc esi ms |
| topic | thymoquinone carvacrol thymol hplc-uv lc-ms/ms stability |
| url | https://doi.org/10.2478/acph-2020-0028 |
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