Establishment of sugarcane mosaic virus-based vector for dual gene expression in maize
Abstract Virus-based gene expression is a simple and powerful approach for functional genetic studies in plants. Here, sugarcane mosaic virus (SCMV, Potyvirus sacchari) was engineered as a dual gene expression vector for simultaneous expression of two heterologous proteins in maize plants. Inoculati...
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| Format: | Article |
| Language: | English |
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BMC
2025-05-01
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| Series: | Phytopathology Research |
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| Online Access: | https://doi.org/10.1186/s42483-025-00330-8 |
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| author | Huangai Li Ke Xie Xiyin Zheng Meng Han Asigul Ismayil Na Liu Xiang Wei Rong Zhong Yan Wang Yule Liu |
| author_facet | Huangai Li Ke Xie Xiyin Zheng Meng Han Asigul Ismayil Na Liu Xiang Wei Rong Zhong Yan Wang Yule Liu |
| author_sort | Huangai Li |
| collection | DOAJ |
| description | Abstract Virus-based gene expression is a simple and powerful approach for functional genetic studies in plants. Here, sugarcane mosaic virus (SCMV, Potyvirus sacchari) was engineered as a dual gene expression vector for simultaneous expression of two heterologous proteins in maize plants. Inoculation of the full-length cDNA clone of SCMV from agro-infiltrated Nicotiana benthamiana resulted in a rapid systemic infection in maize. To assess the possibility of SCMV as the gene expression vector, the marker gene GFP or GUS was inserted into either the NIb/CP or P1/HC-Pro junction site of SCMV to produce single-gene expression vectors. The results showed that these engineered SCMV vectors permitted efficient gene expression in systemically infected leaves and had the genetic capacity of inserts of more than 1800 bp, suggesting that both junction sites are suitable for heterologous gene insertion and expression. Furthermore, two different genes GFP and mCherry could be expressed simultaneously by engineering them into either NIb/CP or P1/HC-Pro junction sites of the same vector. These results clearly demonstrate the suitability of SCMV as a transient dual gene expression vector for maize plants. |
| format | Article |
| id | doaj-art-4fc53d6d994b431284f776a76971ae9a |
| institution | Kabale University |
| issn | 2524-4167 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Phytopathology Research |
| spelling | doaj-art-4fc53d6d994b431284f776a76971ae9a2025-08-20T03:53:11ZengBMCPhytopathology Research2524-41672025-05-017111310.1186/s42483-025-00330-8Establishment of sugarcane mosaic virus-based vector for dual gene expression in maizeHuangai Li0Ke Xie1Xiyin Zheng2Meng Han3Asigul Ismayil4Na Liu5Xiang Wei6Rong Zhong7Yan Wang8Yule Liu9Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityGuangxi Vocational University of AgricultureCenter for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityCenter for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityCenter for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityCenter for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityCenter for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityCenter for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityCenter for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityCenter for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua UniversityAbstract Virus-based gene expression is a simple and powerful approach for functional genetic studies in plants. Here, sugarcane mosaic virus (SCMV, Potyvirus sacchari) was engineered as a dual gene expression vector for simultaneous expression of two heterologous proteins in maize plants. Inoculation of the full-length cDNA clone of SCMV from agro-infiltrated Nicotiana benthamiana resulted in a rapid systemic infection in maize. To assess the possibility of SCMV as the gene expression vector, the marker gene GFP or GUS was inserted into either the NIb/CP or P1/HC-Pro junction site of SCMV to produce single-gene expression vectors. The results showed that these engineered SCMV vectors permitted efficient gene expression in systemically infected leaves and had the genetic capacity of inserts of more than 1800 bp, suggesting that both junction sites are suitable for heterologous gene insertion and expression. Furthermore, two different genes GFP and mCherry could be expressed simultaneously by engineering them into either NIb/CP or P1/HC-Pro junction sites of the same vector. These results clearly demonstrate the suitability of SCMV as a transient dual gene expression vector for maize plants.https://doi.org/10.1186/s42483-025-00330-8Sugarcane mosaic virus (SCMV)MaizeVirus-based gene expression vectorsDual gene expressionMechanical inoculation |
| spellingShingle | Huangai Li Ke Xie Xiyin Zheng Meng Han Asigul Ismayil Na Liu Xiang Wei Rong Zhong Yan Wang Yule Liu Establishment of sugarcane mosaic virus-based vector for dual gene expression in maize Phytopathology Research Sugarcane mosaic virus (SCMV) Maize Virus-based gene expression vectors Dual gene expression Mechanical inoculation |
| title | Establishment of sugarcane mosaic virus-based vector for dual gene expression in maize |
| title_full | Establishment of sugarcane mosaic virus-based vector for dual gene expression in maize |
| title_fullStr | Establishment of sugarcane mosaic virus-based vector for dual gene expression in maize |
| title_full_unstemmed | Establishment of sugarcane mosaic virus-based vector for dual gene expression in maize |
| title_short | Establishment of sugarcane mosaic virus-based vector for dual gene expression in maize |
| title_sort | establishment of sugarcane mosaic virus based vector for dual gene expression in maize |
| topic | Sugarcane mosaic virus (SCMV) Maize Virus-based gene expression vectors Dual gene expression Mechanical inoculation |
| url | https://doi.org/10.1186/s42483-025-00330-8 |
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