Genes related to neural tube defects and glioblastoma
Abstract There are many similarities between early embryonic development and tumorigenesis. The occurrence of neural tube defects (NTDs) and glioblastoma (GBM) are both related to the abnormal development of neuroectodermal cells. To obtain genes related to both NTDs and GBM, as well as small molecu...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-86891-2 |
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| author | Rui Cao Yurong Liu Kaixin Wei Ning Jin Yuxiang Liang Ruifang Ao Weiwei Pan Xiang Wang Xiuwei Wang Li Zhang Jun Xie |
| author_facet | Rui Cao Yurong Liu Kaixin Wei Ning Jin Yuxiang Liang Ruifang Ao Weiwei Pan Xiang Wang Xiuwei Wang Li Zhang Jun Xie |
| author_sort | Rui Cao |
| collection | DOAJ |
| description | Abstract There are many similarities between early embryonic development and tumorigenesis. The occurrence of neural tube defects (NTDs) and glioblastoma (GBM) are both related to the abnormal development of neuroectodermal cells. To obtain genes related to both NTDs and GBM, as well as small molecule drugs with potential clinical application value. We performed bioinformatics analysis on transcriptome sequencing data of retinoic acid (RA)-induced NTDs mice, human NTDs samples and GBM samples. RT-qPCR, Western blot, and immunohistochemistry were used to validate the expression of candidate genes. Our results indicated that two genes at mRNA and protein levels have been well verified in both NTDs mouse and GBM human samples, namely, Poli and Fgf1. Molecular docking and validating in vitro were performed for FGF1 against pazopanib by using Autodock and Biacore. Cytological experiments showed that pazopanib significantly inhibited the proliferation of GBM tumor cells and mouse neural cells, promoted apoptosis, and had no effect on GBM tumor cells migration. Overall, our results demonstrated that Fgf1 abnormally expressed at different developmental stages, it may be a potentially prenatal biomarker for NTDs and potential therapeutic target for GBM. Pazopanib may be a new drug for the treatment of GBM tumors. |
| format | Article |
| id | doaj-art-4fc1396ccc4a44a396d2917b2b0b029a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4fc1396ccc4a44a396d2917b2b0b029a2025-02-02T12:22:59ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-025-86891-2Genes related to neural tube defects and glioblastomaRui Cao0Yurong Liu1Kaixin Wei2Ning Jin3Yuxiang Liang4Ruifang Ao5Weiwei Pan6Xiang Wang7Xiuwei Wang8Li Zhang9Jun Xie10Department of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Key Laboratory of Coal Environmental Pathogenicity and Prevention (Ministry of Education, China, Shanxi Medical UniversityAcademy of Medical Sciences, Shanxi Medical UniversityDepartment of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Key Laboratory of Coal Environmental Pathogenicity and Prevention (Ministry of Education, China, Shanxi Medical UniversityDepartment of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Key Laboratory of Coal Environmental Pathogenicity and Prevention (Ministry of Education, China, Shanxi Medical UniversityDepartment of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Key Laboratory of Coal Environmental Pathogenicity and Prevention (Ministry of Education, China, Shanxi Medical UniversityDepartment of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Key Laboratory of Coal Environmental Pathogenicity and Prevention (Ministry of Education, China, Shanxi Medical UniversityShanxi Key Laboratory of Pharmaceutical Biotechnology, Shanxi Biological Research Institute Co., LtdShanxi Key Laboratory of Pharmaceutical Biotechnology, Shanxi Biological Research Institute Co., LtdBeijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of PediatricsDepartment of Hepatobiliary Surgery and Liver Transplant Center, The First Hospital of Shanxi Medical UniversityDepartment of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Key Laboratory of Coal Environmental Pathogenicity and Prevention (Ministry of Education, China, Shanxi Medical UniversityAbstract There are many similarities between early embryonic development and tumorigenesis. The occurrence of neural tube defects (NTDs) and glioblastoma (GBM) are both related to the abnormal development of neuroectodermal cells. To obtain genes related to both NTDs and GBM, as well as small molecule drugs with potential clinical application value. We performed bioinformatics analysis on transcriptome sequencing data of retinoic acid (RA)-induced NTDs mice, human NTDs samples and GBM samples. RT-qPCR, Western blot, and immunohistochemistry were used to validate the expression of candidate genes. Our results indicated that two genes at mRNA and protein levels have been well verified in both NTDs mouse and GBM human samples, namely, Poli and Fgf1. Molecular docking and validating in vitro were performed for FGF1 against pazopanib by using Autodock and Biacore. Cytological experiments showed that pazopanib significantly inhibited the proliferation of GBM tumor cells and mouse neural cells, promoted apoptosis, and had no effect on GBM tumor cells migration. Overall, our results demonstrated that Fgf1 abnormally expressed at different developmental stages, it may be a potentially prenatal biomarker for NTDs and potential therapeutic target for GBM. Pazopanib may be a new drug for the treatment of GBM tumors.https://doi.org/10.1038/s41598-025-86891-2Neural tube defectsGlioblastoma, Fgf1Pazopanib |
| spellingShingle | Rui Cao Yurong Liu Kaixin Wei Ning Jin Yuxiang Liang Ruifang Ao Weiwei Pan Xiang Wang Xiuwei Wang Li Zhang Jun Xie Genes related to neural tube defects and glioblastoma Scientific Reports Neural tube defects Glioblastoma, Fgf1 Pazopanib |
| title | Genes related to neural tube defects and glioblastoma |
| title_full | Genes related to neural tube defects and glioblastoma |
| title_fullStr | Genes related to neural tube defects and glioblastoma |
| title_full_unstemmed | Genes related to neural tube defects and glioblastoma |
| title_short | Genes related to neural tube defects and glioblastoma |
| title_sort | genes related to neural tube defects and glioblastoma |
| topic | Neural tube defects Glioblastoma, Fgf1 Pazopanib |
| url | https://doi.org/10.1038/s41598-025-86891-2 |
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