Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida
Abstract The jewel wasp, Nasonia (Hymenoptera: Pteromalidae), is a well-established model system for evolutionary genetics and host-microbial interactions. Here, we present the genome of N. oneida, a species lacking prior genomic characterization, using 10× Genomics linked-read (400× coverage), Illu...
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| Format: | Article |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05375-0 |
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| author | Anjali Rana Paras Verma Shashi Bhushan Pandit John H. Werren Xu Wang Rhitoban Raychoudhury |
| author_facet | Anjali Rana Paras Verma Shashi Bhushan Pandit John H. Werren Xu Wang Rhitoban Raychoudhury |
| author_sort | Anjali Rana |
| collection | DOAJ |
| description | Abstract The jewel wasp, Nasonia (Hymenoptera: Pteromalidae), is a well-established model system for evolutionary genetics and host-microbial interactions. Here, we present the genome of N. oneida, a species lacking prior genomic characterization, using 10× Genomics linked-read (400× coverage), Illumina short-read (120× coverage), and transcriptome data (30× coverage). The assembled genome size is 267 Mb, comprising 4,675 scaffolds, with a scaffold N50 of 1 Mb and 98.40% Benchmarking Universal Single-Copy Orthologues (BUSCOs) completeness score. Annotation revealed 32.29% (86.46 Mb) of repetitive sequences and 14,221 protein-coding genes. Comparative genomics of N. oneida with 15 other hymenopteran species validated the presence of 5,939 gene families shared among them, including 3643 single-copy and 2296 multicopy gene families. This study provides the first de novo assembly of N. oneida, providing a significant addition to the growing repertoire of molecular tools for comparative genomics and functional studies to understand the evolution of closely related species as well as the evolution of parasitic wasps. |
| format | Article |
| id | doaj-art-4fba6f422ff44db88b770b9dfbc6b7e2 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Data |
| spelling | doaj-art-4fba6f422ff44db88b770b9dfbc6b7e22025-08-20T03:45:27ZengNature PortfolioScientific Data2052-44632025-07-0112111110.1038/s41597-025-05375-0Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneidaAnjali Rana0Paras Verma1Shashi Bhushan Pandit2John H. Werren3Xu Wang4Rhitoban Raychoudhury5Indian Institute of Science Education and Research (IISER Mohali)Indian Institute of Science Education and Research (IISER Mohali)Indian Institute of Science Education and Research (IISER Mohali)Department of Biology, University of RochesterDepartment of Pathobiology, College of Veterinary Medicine, Auburn UniversityIndian Institute of Science Education and Research (IISER Mohali)Abstract The jewel wasp, Nasonia (Hymenoptera: Pteromalidae), is a well-established model system for evolutionary genetics and host-microbial interactions. Here, we present the genome of N. oneida, a species lacking prior genomic characterization, using 10× Genomics linked-read (400× coverage), Illumina short-read (120× coverage), and transcriptome data (30× coverage). The assembled genome size is 267 Mb, comprising 4,675 scaffolds, with a scaffold N50 of 1 Mb and 98.40% Benchmarking Universal Single-Copy Orthologues (BUSCOs) completeness score. Annotation revealed 32.29% (86.46 Mb) of repetitive sequences and 14,221 protein-coding genes. Comparative genomics of N. oneida with 15 other hymenopteran species validated the presence of 5,939 gene families shared among them, including 3643 single-copy and 2296 multicopy gene families. This study provides the first de novo assembly of N. oneida, providing a significant addition to the growing repertoire of molecular tools for comparative genomics and functional studies to understand the evolution of closely related species as well as the evolution of parasitic wasps.https://doi.org/10.1038/s41597-025-05375-0 |
| spellingShingle | Anjali Rana Paras Verma Shashi Bhushan Pandit John H. Werren Xu Wang Rhitoban Raychoudhury Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida Scientific Data |
| title | Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida |
| title_full | Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida |
| title_fullStr | Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida |
| title_full_unstemmed | Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida |
| title_short | Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida |
| title_sort | genome assembly and annotation of the parasitoid jewel wasp nasonia oneida |
| url | https://doi.org/10.1038/s41597-025-05375-0 |
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