Ha-ras Oncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells
When transfected to benzo[a]pyrene (BP)‐transformed MCF‐10F human breast epithelial cells (BP1 cell line) the c‐Ha‐ras oncogene has proven to enhance the neoplastic changes initiated by exposure to BP, giving rise to an aggressive tumorigenic cell line, BP1‐Tras. We have previously demonstrated by i...
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| Format: | Article |
| Language: | English |
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Wiley
1999-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1999/903790 |
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| author | Maria Luiza S. Mello Benedicto de Campos Vidal Jose Russo |
| author_facet | Maria Luiza S. Mello Benedicto de Campos Vidal Jose Russo |
| author_sort | Maria Luiza S. Mello |
| collection | DOAJ |
| description | When transfected to benzo[a]pyrene (BP)‐transformed MCF‐10F human breast epithelial cells (BP1 cell line) the c‐Ha‐ras oncogene has proven to enhance the neoplastic changes initiated by exposure to BP, giving rise to an aggressive tumorigenic cell line, BP1‐Tras. We have previously demonstrated by image analysis that BP affects the DNA content and the chromatin supraorganization of MCF‐10F cells. Here Feulgen‐stained BP1‐Tras cells were studied by image analysis in order to evaluate possible additional changes in DNA content and chromatin texture induced by insertion of the ras oncogene. A high variability in DNA content also including polyploidy or near‐polyploidy, and an increase in the packing states of the chromatin which became still condensed in BP1 cells were found in BP1‐Tras cells. The results differed from those reported for the BP1‐E1 cell line which is also an aggressive tumorigenic cell line, but was attained through progressive passages of BP‐transformed cells. It was demonstrated that different patterns of changes in DNA content and chromatin organization may be involved in equally aggressive tumorigenic BP‐transformed cell lines originated from the same cell line by different mechanisms. |
| format | Article |
| id | doaj-art-4fb8ffbdfc0646d78a0a87f25d113aee |
| institution | OA Journals |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-4fb8ffbdfc0646d78a0a87f25d113aee2025-08-20T02:05:38ZengWileyAnalytical Cellular Pathology0921-89121878-36511999-01-01192737910.1155/1999/903790Ha-ras Oncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial CellsMaria Luiza S. Mello0Benedicto de Campos Vidal1Jose Russo2Department of Cell Biology, Institute of Biology, UNICAMP, 13083‐970 Campinas, SP, BrazilDepartment of Cell Biology, Institute of Biology, UNICAMP, 13083‐970 Campinas, SP, BrazilBreast Cancer Research Laboratory, Fox Chase Cancer Center, Philadelphia, PA19111, USAWhen transfected to benzo[a]pyrene (BP)‐transformed MCF‐10F human breast epithelial cells (BP1 cell line) the c‐Ha‐ras oncogene has proven to enhance the neoplastic changes initiated by exposure to BP, giving rise to an aggressive tumorigenic cell line, BP1‐Tras. We have previously demonstrated by image analysis that BP affects the DNA content and the chromatin supraorganization of MCF‐10F cells. Here Feulgen‐stained BP1‐Tras cells were studied by image analysis in order to evaluate possible additional changes in DNA content and chromatin texture induced by insertion of the ras oncogene. A high variability in DNA content also including polyploidy or near‐polyploidy, and an increase in the packing states of the chromatin which became still condensed in BP1 cells were found in BP1‐Tras cells. The results differed from those reported for the BP1‐E1 cell line which is also an aggressive tumorigenic cell line, but was attained through progressive passages of BP‐transformed cells. It was demonstrated that different patterns of changes in DNA content and chromatin organization may be involved in equally aggressive tumorigenic BP‐transformed cell lines originated from the same cell line by different mechanisms.http://dx.doi.org/10.1155/1999/903790 |
| spellingShingle | Maria Luiza S. Mello Benedicto de Campos Vidal Jose Russo Ha-ras Oncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells Analytical Cellular Pathology |
| title | Ha-ras Oncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells |
| title_full | Ha-ras Oncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells |
| title_fullStr | Ha-ras Oncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells |
| title_full_unstemmed | Ha-ras Oncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells |
| title_short | Ha-ras Oncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells |
| title_sort | ha ras oncogene effect on dna content and chromatin supraorganization in benzo a pyrene transformed human breast epithelial cells |
| url | http://dx.doi.org/10.1155/1999/903790 |
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