Chromosome-level genome assembly of an Arctic fish species pale eelpout (Lycodes pallidus)

Abstract Eelpouts (Zoarcidae) are known for their bipolar distributions and distinctive biogeographic histories. However, limited genomic data have hindered our understanding of their adaptive evolution. In this study, we present a thoroughly annotated chromosome-level genome assembly of pale eelpou...

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Main Authors: Ran Zhang, Rui Wang, Xing Miao, Hai Li, Puqing Song, Yuan Li, Longshan Lin
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05385-y
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author Ran Zhang
Rui Wang
Xing Miao
Hai Li
Puqing Song
Yuan Li
Longshan Lin
author_facet Ran Zhang
Rui Wang
Xing Miao
Hai Li
Puqing Song
Yuan Li
Longshan Lin
author_sort Ran Zhang
collection DOAJ
description Abstract Eelpouts (Zoarcidae) are known for their bipolar distributions and distinctive biogeographic histories. However, limited genomic data have hindered our understanding of their adaptive evolution. In this study, we present a thoroughly annotated chromosome-level genome assembly of pale eelpout (Lycodes pallidus) generated through the integration of Illumina, PacBio circular consensus, and Hi-C sequencing techniques. The final assembly spans 753.4 Mb, with its high quality confirmed by a scaffold N50 of 28.6 Mb and a Benchmarking Universal Single-Copy Ortholog (BUSCO) completeness of 99.3%. In comparison to other eelpouts and related fishes, the L. pallidus genome is larger and exhibits greater repetitive element content, accounting for approximately 45% of its total length. We annotated 21,419 protein-coding genes, a significant proportion of which are involved in signal transduction mechanisms and transcription. These findings provide valuable genetic resources for elucidating the evolutionary mechanisms underlying polar fish adaptation.
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id doaj-art-4fb03588f1e343ffa098037eee818a0d
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publishDate 2025-07-01
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spelling doaj-art-4fb03588f1e343ffa098037eee818a0d2025-08-20T03:04:18ZengNature PortfolioScientific Data2052-44632025-07-011211810.1038/s41597-025-05385-yChromosome-level genome assembly of an Arctic fish species pale eelpout (Lycodes pallidus)Ran Zhang0Rui Wang1Xing Miao2Hai Li3Puqing Song4Yuan Li5Longshan Lin6Laboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural ResourcesLaboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural ResourcesLaboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural ResourcesLaboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural ResourcesLaboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural ResourcesLaboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural ResourcesLaboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural ResourcesAbstract Eelpouts (Zoarcidae) are known for their bipolar distributions and distinctive biogeographic histories. However, limited genomic data have hindered our understanding of their adaptive evolution. In this study, we present a thoroughly annotated chromosome-level genome assembly of pale eelpout (Lycodes pallidus) generated through the integration of Illumina, PacBio circular consensus, and Hi-C sequencing techniques. The final assembly spans 753.4 Mb, with its high quality confirmed by a scaffold N50 of 28.6 Mb and a Benchmarking Universal Single-Copy Ortholog (BUSCO) completeness of 99.3%. In comparison to other eelpouts and related fishes, the L. pallidus genome is larger and exhibits greater repetitive element content, accounting for approximately 45% of its total length. We annotated 21,419 protein-coding genes, a significant proportion of which are involved in signal transduction mechanisms and transcription. These findings provide valuable genetic resources for elucidating the evolutionary mechanisms underlying polar fish adaptation.https://doi.org/10.1038/s41597-025-05385-y
spellingShingle Ran Zhang
Rui Wang
Xing Miao
Hai Li
Puqing Song
Yuan Li
Longshan Lin
Chromosome-level genome assembly of an Arctic fish species pale eelpout (Lycodes pallidus)
Scientific Data
title Chromosome-level genome assembly of an Arctic fish species pale eelpout (Lycodes pallidus)
title_full Chromosome-level genome assembly of an Arctic fish species pale eelpout (Lycodes pallidus)
title_fullStr Chromosome-level genome assembly of an Arctic fish species pale eelpout (Lycodes pallidus)
title_full_unstemmed Chromosome-level genome assembly of an Arctic fish species pale eelpout (Lycodes pallidus)
title_short Chromosome-level genome assembly of an Arctic fish species pale eelpout (Lycodes pallidus)
title_sort chromosome level genome assembly of an arctic fish species pale eelpout lycodes pallidus
url https://doi.org/10.1038/s41597-025-05385-y
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