Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows
Background and Aim: Milk physicochemical properties play essential role in the milk processing industry, which are moderately to highly affected by genetic factors. This study aimed to evaluate the association between single nucleotide polymorphisms in POU class 1 homeobox 1 (POU1F1) and the physico...
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Veterinary World
2024-10-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.17/October-2024/12.pdf |
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| author | Muhammad Cahyadi Ammar Ibnu Hasan Djorodjatun Samodro Sakti Nasta ‘Ainun Nissa Ahmad Pramono Suryo Firmanto Rizwan Nur Friyatna Slamet Diah Volkandari Pita Sudrajad |
| author_facet | Muhammad Cahyadi Ammar Ibnu Hasan Djorodjatun Samodro Sakti Nasta ‘Ainun Nissa Ahmad Pramono Suryo Firmanto Rizwan Nur Friyatna Slamet Diah Volkandari Pita Sudrajad |
| author_sort | Muhammad Cahyadi |
| collection | DOAJ |
| description | Background and Aim: Milk physicochemical properties play essential role in the milk processing industry, which are moderately to highly affected by genetic factors. This study aimed to evaluate the association between single nucleotide polymorphisms in POU class 1 homeobox 1 (POU1F1) and the physicochemical properties of milk in high-producing Holstein Friesian (HF) cows.
Materials and Methods: A total of 149 high-producing dairy cows from PT Ultra Peternakan Bandung Selatan was included in this study. The physicochemical properties of milk, including density, freezing point, pH, lactose, solid non-fat, protein, and ash content, were determined. Moreover, three polymorphisms within the exon regions of POU1F1 (c.195G>A, c.300G>T, and c.828G>A) were analyzed using polymerase chain reaction-restriction fragment length polymorphism. The association between these polymorphisms and the physicochemical properties of milk was determined using a mixed-effects model analysis, in which the lactation period was used as a covariate.
Results: This study found that two polymorphisms, c.195G>A and c.828G>A, significantly affected the pH of fresh milk. Cows with both the GG genotypes c.195G>A and c.828G>A had lower milk pH values than those with the other genotypes. In addition, a non-significant effect of POU1F1 was observed on the other physicochemical properties of milk.
Conclusion: Two POU1F1 polymorphisms determined the pH of fresh milk in the Indonesian HF population. These are potential marker candidates for milk pH that directly affect the development of dairy products in the milk processing industry. |
| format | Article |
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| institution | OA Journals |
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| language | English |
| publishDate | 2024-10-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj-art-4fafcd1accc44c9e8e5091bf4df314f92025-08-20T02:08:43ZengVeterinary WorldVeterinary World0972-89882231-09162024-10-0117102304231010.14202/vetworld.2024.2304-2310Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cowsMuhammad Cahyadi0https://orcid.org/0000-0003-3300-6479Ammar Ibnu Hasan1https://orcid.org/0009-0007-8127-5987Djorodjatun Samodro Sakti2https://orcid.org/0000-0003-2639-4493Nasta ‘Ainun Nissa3https://orcid.org/0009-0005-1674-3137Ahmad Pramono4https://orcid.org/0000-0001-6915-9456Suryo Firmanto5https://orcid.org/0009-0006-8361-4615Rizwan Nur Friyatna6https://orcid.org/0009-0006-2529-2363Slamet Diah Volkandari7https://orcid.org/0000-0002-4676-4028Pita Sudrajad8https://orcid.org/0000-0001-8148-9095Department of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, Indonesia.Department of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, Indonesia.Department of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, Indonesia.Department of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, Indonesia.Department of Animal Science, Universitas Sebelas Maret, Surakarta, Jawa Tengah 57126, Indonesia.PT Ultra Peternakan Bandung Selatan, Pangalengan, Kabupaten Bandung, Jawa Barat 40378, Indonesia.PT Ultra Peternakan Bandung Selatan, Pangalengan, Kabupaten Bandung, Jawa Barat 40378, Indonesia.Research Center for Food Technology and Processing, The Indonesian National Research and Innovation Agency, Gunung Kidul, Daerah Istimewa Yogyakarta 55861, Indonesia.Research Center for Animal Husbandry, The Indonesian National Research and Innovation Agency, Bogor, Jawa Barat - 16911, Indonesia. Background and Aim: Milk physicochemical properties play essential role in the milk processing industry, which are moderately to highly affected by genetic factors. This study aimed to evaluate the association between single nucleotide polymorphisms in POU class 1 homeobox 1 (POU1F1) and the physicochemical properties of milk in high-producing Holstein Friesian (HF) cows. Materials and Methods: A total of 149 high-producing dairy cows from PT Ultra Peternakan Bandung Selatan was included in this study. The physicochemical properties of milk, including density, freezing point, pH, lactose, solid non-fat, protein, and ash content, were determined. Moreover, three polymorphisms within the exon regions of POU1F1 (c.195G>A, c.300G>T, and c.828G>A) were analyzed using polymerase chain reaction-restriction fragment length polymorphism. The association between these polymorphisms and the physicochemical properties of milk was determined using a mixed-effects model analysis, in which the lactation period was used as a covariate. Results: This study found that two polymorphisms, c.195G>A and c.828G>A, significantly affected the pH of fresh milk. Cows with both the GG genotypes c.195G>A and c.828G>A had lower milk pH values than those with the other genotypes. In addition, a non-significant effect of POU1F1 was observed on the other physicochemical properties of milk. Conclusion: Two POU1F1 polymorphisms determined the pH of fresh milk in the Indonesian HF population. These are potential marker candidates for milk pH that directly affect the development of dairy products in the milk processing industry.https://www.veterinaryworld.org/Vol.17/October-2024/12.pdfexonic mutationholstein friesianmilk phphysicochemical propertiespou1f1 |
| spellingShingle | Muhammad Cahyadi Ammar Ibnu Hasan Djorodjatun Samodro Sakti Nasta ‘Ainun Nissa Ahmad Pramono Suryo Firmanto Rizwan Nur Friyatna Slamet Diah Volkandari Pita Sudrajad Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows Veterinary World exonic mutation holstein friesian milk ph physicochemical properties pou1f1 |
| title | Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows |
| title_full | Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows |
| title_fullStr | Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows |
| title_full_unstemmed | Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows |
| title_short | Exonic mutations of POU class 1 homeobox 1 are associated with milk pH in high-producing Holstein Friesian cows |
| title_sort | exonic mutations of pou class 1 homeobox 1 are associated with milk ph in high producing holstein friesian cows |
| topic | exonic mutation holstein friesian milk ph physicochemical properties pou1f1 |
| url | https://www.veterinaryworld.org/Vol.17/October-2024/12.pdf |
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