Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired
BackgroundPsoriasis is a systemic, immune-mediated, inflammatory skin disease in which T cells have been found to play a significant role. The phenotypic and functional properties of circulating CD8 T cells in the pathogenesis of the disease are still ill-defined.ObjectiveThis study aimed to assess...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1585378/full |
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| author | Yiqiao Chen Chiara Tontini Isabella Tosi Jonathan N. Barker Paola Di Meglio Christopher E. M. Griffiths Christopher E. M. Griffiths Rajia Bahri Silvia Bulfone-Paus |
| author_facet | Yiqiao Chen Chiara Tontini Isabella Tosi Jonathan N. Barker Paola Di Meglio Christopher E. M. Griffiths Christopher E. M. Griffiths Rajia Bahri Silvia Bulfone-Paus |
| author_sort | Yiqiao Chen |
| collection | DOAJ |
| description | BackgroundPsoriasis is a systemic, immune-mediated, inflammatory skin disease in which T cells have been found to play a significant role. The phenotypic and functional properties of circulating CD8 T cells in the pathogenesis of the disease are still ill-defined.ObjectiveThis study aimed to assess changes in the phenotype, activation status and mediator release of CD8 T cells in the peripheral blood of patients with mild-to-moderate psoriasis.MethodsPeripheral blood mononuclear cells from patients with mild-to-moderate psoriasis and healthy individuals were used to investigate the CD8 T cell immune phenotype and mediator release upon in vitro TCR-independent (phorbol 12-myristate 13-acetate (PMA) plus ionomycin (ION)) or TCR-dependent (anti-CD3/CD28) activation by flow cytometry.ResultsPatients with psoriasis exhibited reduced circulating CD8 memory T cell frequency compared to healthy controls. Additionally, although CD8 T cell subsets showed similar levels of the skin homing marker CCR4, they demonstrated a significant upregulation of B- and T-lymphocyte attenuator (BTLA) expression compared to healthy individuals. Upon CD8 T cell activation, IL-17A and IL-17F were expressed at low and comparable levels in psoriasis patients and healthy controls. In contrast, CD69, IFNγ, and Granzyme B were significantly decreased in anti-CD3/CD28-activated CD8 T cell subsets. PASI scores positively correlated with IFNγ-producing CD8 T memory cells and negatively with TNF-producing CD8- T cell subsets.ConclusionPatients with mild-to-moderate psoriasis showed a significant decrease in CD8 T memory cells and reduced release of cytotoxic mediators by CD8 T cells. Thus, this indicates that psoriasis impacts the functionality of circulating CD8 T cells. |
| format | Article |
| id | doaj-art-4f8cc0e177ca47f2ae924059e6fe9f63 |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-4f8cc0e177ca47f2ae924059e6fe9f632025-08-20T03:10:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-05-011610.3389/fimmu.2025.15853781585378Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impairedYiqiao Chen0Chiara Tontini1Isabella Tosi2Jonathan N. Barker3Paola Di Meglio4Christopher E. M. Griffiths5Christopher E. M. Griffiths6Rajia Bahri7Silvia Bulfone-Paus8Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United KingdomLydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United KingdomSt. John’s Institute of Dermatology, School of Basic and Medical Biosciences, King’s College London, London, United KingdomSt. John’s Institute of Dermatology, School of Basic and Medical Biosciences, King’s College London, London, United KingdomSt. John’s Institute of Dermatology, School of Basic and Medical Biosciences, King’s College London, London, United KingdomDermatology Centre, Manchester Academic Health Science Centre, Northern Care Alliance NHS Foundation Trust, Manchester, United KingdomDepartment of Dermatology, King’s College Hospital, King’s College London, London, United KingdomLydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United KingdomLydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United KingdomBackgroundPsoriasis is a systemic, immune-mediated, inflammatory skin disease in which T cells have been found to play a significant role. The phenotypic and functional properties of circulating CD8 T cells in the pathogenesis of the disease are still ill-defined.ObjectiveThis study aimed to assess changes in the phenotype, activation status and mediator release of CD8 T cells in the peripheral blood of patients with mild-to-moderate psoriasis.MethodsPeripheral blood mononuclear cells from patients with mild-to-moderate psoriasis and healthy individuals were used to investigate the CD8 T cell immune phenotype and mediator release upon in vitro TCR-independent (phorbol 12-myristate 13-acetate (PMA) plus ionomycin (ION)) or TCR-dependent (anti-CD3/CD28) activation by flow cytometry.ResultsPatients with psoriasis exhibited reduced circulating CD8 memory T cell frequency compared to healthy controls. Additionally, although CD8 T cell subsets showed similar levels of the skin homing marker CCR4, they demonstrated a significant upregulation of B- and T-lymphocyte attenuator (BTLA) expression compared to healthy individuals. Upon CD8 T cell activation, IL-17A and IL-17F were expressed at low and comparable levels in psoriasis patients and healthy controls. In contrast, CD69, IFNγ, and Granzyme B were significantly decreased in anti-CD3/CD28-activated CD8 T cell subsets. PASI scores positively correlated with IFNγ-producing CD8 T memory cells and negatively with TNF-producing CD8- T cell subsets.ConclusionPatients with mild-to-moderate psoriasis showed a significant decrease in CD8 T memory cells and reduced release of cytotoxic mediators by CD8 T cells. Thus, this indicates that psoriasis impacts the functionality of circulating CD8 T cells.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1585378/fullflow cytometryCD8 T cellsmultiparametric analysisunsupervised clusteringimmunophenotypingperipheral blood mononuclear cells |
| spellingShingle | Yiqiao Chen Chiara Tontini Isabella Tosi Jonathan N. Barker Paola Di Meglio Christopher E. M. Griffiths Christopher E. M. Griffiths Rajia Bahri Silvia Bulfone-Paus Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired Frontiers in Immunology flow cytometry CD8 T cells multiparametric analysis unsupervised clustering immunophenotyping peripheral blood mononuclear cells |
| title | Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired |
| title_full | Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired |
| title_fullStr | Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired |
| title_full_unstemmed | Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired |
| title_short | Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired |
| title_sort | circulating cd8 t cells from patients with mild to moderate psoriasis are functionally impaired |
| topic | flow cytometry CD8 T cells multiparametric analysis unsupervised clustering immunophenotyping peripheral blood mononuclear cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1585378/full |
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