Bacterial Infection Disrupts the Intestinal Bacterial Community and Facilitates the Enrichment of Pathogenic Bacteria in the Intestines of <i>Penaeus vannamei</i>

Pathogenic infections can reshape the intestinal microbiota of aquatic animals, thereby impacting their health status. In this study, we aimed to investigate whether <i>Vibrio parahaemolyticus</i> infection induces dysbiosis in the intestinal bacterial community of <i>Penaeus vanna...

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Main Authors: Renjun Zhou, Shaoping Weng, Jianguo He
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/4/864
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author Renjun Zhou
Shaoping Weng
Jianguo He
author_facet Renjun Zhou
Shaoping Weng
Jianguo He
author_sort Renjun Zhou
collection DOAJ
description Pathogenic infections can reshape the intestinal microbiota of aquatic animals, thereby impacting their health status. In this study, we aimed to investigate whether <i>Vibrio parahaemolyticus</i> infection induces dysbiosis in the intestinal bacterial community of <i>Penaeus vannamei</i> and to assess the associated ecological risks. Our findings revealed the deterministic processes in intestinal bacterial community assembly during bacterial infections, indicating that host selection, i.e., host immune response post-infection, has a significant influence on intestinal microbes. More importantly, we found that bacterial infection reshaped the intestinal community by reducing the relative abundance of probiotic <i>Ruegeria</i> species (e.g., <i>R. atlantica</i>, <i>R. lacuscaerulensis</i>, <i>R. conchae</i>, <i>R. profundi</i>, <i>R. arenilitoris</i>, <i>R. pomeroyi</i>) and increasing the relative abundance of <i>Vibrio</i> species (<i>V. harveyi</i>, <i>V. sinaloensis</i>, <i>V. coralliilyticus</i>, and <i>V. brasiliensis</i>). Significant negative correlations were observed between the relative abundance of these <i>Ruegeria</i> species and the relative abundance of <i>Vibrio</i> species. Moreover, the control <i>P. vannamei</i> contained a substantially higher number of keystone species belonging to <i>Ruegeria</i> in the bacterial community network, whereas bacterial infection individuals had few or no keystone species belonging to <i>Ruegeria</i>, with keystone species belonging to <i>Vibrio</i> becoming more prominent. Thus, the significant increase in <i>Vibrio</i> species abundance in the <i>P. vannamei</i> intestine following bacterial infection was associated with the marked reduction in <i>Ruegeria</i> species. Our findings will provide valuable insights into the complex interactions among bacterial infection, intestinal microbiota, and host health, and they provide guidance for the development of probiotics in promoting the healthy culture of <i>P. vannamei</i>.
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spelling doaj-art-4f8a0c82987a406a9b8a935318732f9e2025-08-20T02:18:04ZengMDPI AGMicroorganisms2076-26072025-04-0113486410.3390/microorganisms13040864Bacterial Infection Disrupts the Intestinal Bacterial Community and Facilitates the Enrichment of Pathogenic Bacteria in the Intestines of <i>Penaeus vannamei</i>Renjun Zhou0Shaoping Weng1Jianguo He2State Key Laboratory of Biocontrol/School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol/School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol/School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaPathogenic infections can reshape the intestinal microbiota of aquatic animals, thereby impacting their health status. In this study, we aimed to investigate whether <i>Vibrio parahaemolyticus</i> infection induces dysbiosis in the intestinal bacterial community of <i>Penaeus vannamei</i> and to assess the associated ecological risks. Our findings revealed the deterministic processes in intestinal bacterial community assembly during bacterial infections, indicating that host selection, i.e., host immune response post-infection, has a significant influence on intestinal microbes. More importantly, we found that bacterial infection reshaped the intestinal community by reducing the relative abundance of probiotic <i>Ruegeria</i> species (e.g., <i>R. atlantica</i>, <i>R. lacuscaerulensis</i>, <i>R. conchae</i>, <i>R. profundi</i>, <i>R. arenilitoris</i>, <i>R. pomeroyi</i>) and increasing the relative abundance of <i>Vibrio</i> species (<i>V. harveyi</i>, <i>V. sinaloensis</i>, <i>V. coralliilyticus</i>, and <i>V. brasiliensis</i>). Significant negative correlations were observed between the relative abundance of these <i>Ruegeria</i> species and the relative abundance of <i>Vibrio</i> species. Moreover, the control <i>P. vannamei</i> contained a substantially higher number of keystone species belonging to <i>Ruegeria</i> in the bacterial community network, whereas bacterial infection individuals had few or no keystone species belonging to <i>Ruegeria</i>, with keystone species belonging to <i>Vibrio</i> becoming more prominent. Thus, the significant increase in <i>Vibrio</i> species abundance in the <i>P. vannamei</i> intestine following bacterial infection was associated with the marked reduction in <i>Ruegeria</i> species. Our findings will provide valuable insights into the complex interactions among bacterial infection, intestinal microbiota, and host health, and they provide guidance for the development of probiotics in promoting the healthy culture of <i>P. vannamei</i>.https://www.mdpi.com/2076-2607/13/4/864<i>Penaeus vannamei</i>bacterial infection<i>Vibrio parahaemolyticus</i>intestine microbiota<i>Ruegeria</i>
spellingShingle Renjun Zhou
Shaoping Weng
Jianguo He
Bacterial Infection Disrupts the Intestinal Bacterial Community and Facilitates the Enrichment of Pathogenic Bacteria in the Intestines of <i>Penaeus vannamei</i>
Microorganisms
<i>Penaeus vannamei</i>
bacterial infection
<i>Vibrio parahaemolyticus</i>
intestine microbiota
<i>Ruegeria</i>
title Bacterial Infection Disrupts the Intestinal Bacterial Community and Facilitates the Enrichment of Pathogenic Bacteria in the Intestines of <i>Penaeus vannamei</i>
title_full Bacterial Infection Disrupts the Intestinal Bacterial Community and Facilitates the Enrichment of Pathogenic Bacteria in the Intestines of <i>Penaeus vannamei</i>
title_fullStr Bacterial Infection Disrupts the Intestinal Bacterial Community and Facilitates the Enrichment of Pathogenic Bacteria in the Intestines of <i>Penaeus vannamei</i>
title_full_unstemmed Bacterial Infection Disrupts the Intestinal Bacterial Community and Facilitates the Enrichment of Pathogenic Bacteria in the Intestines of <i>Penaeus vannamei</i>
title_short Bacterial Infection Disrupts the Intestinal Bacterial Community and Facilitates the Enrichment of Pathogenic Bacteria in the Intestines of <i>Penaeus vannamei</i>
title_sort bacterial infection disrupts the intestinal bacterial community and facilitates the enrichment of pathogenic bacteria in the intestines of i penaeus vannamei i
topic <i>Penaeus vannamei</i>
bacterial infection
<i>Vibrio parahaemolyticus</i>
intestine microbiota
<i>Ruegeria</i>
url https://www.mdpi.com/2076-2607/13/4/864
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