Increased expression of CSF1 in patients with eosinophilic asthma
Abstract Background The link between colony‐stimulating factor 1 (CSF1) and asthma was reported recently. However, the role and mechanism of CSF1 in asthma remain poorly understood. In this study, we aimed to explore the expression and its potential mechanism of CSF1 in asthma. Methods CSF1 expressi...
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.847 |
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| author | Lijuan Du Lu Tang Lisha Xiao Kun Tang Zhimin Zeng Yuxia Liang Yubiao Guo |
| author_facet | Lijuan Du Lu Tang Lisha Xiao Kun Tang Zhimin Zeng Yuxia Liang Yubiao Guo |
| author_sort | Lijuan Du |
| collection | DOAJ |
| description | Abstract Background The link between colony‐stimulating factor 1 (CSF1) and asthma was reported recently. However, the role and mechanism of CSF1 in asthma remain poorly understood. In this study, we aimed to explore the expression and its potential mechanism of CSF1 in asthma. Methods CSF1 expression in the airway samples from asthmatics and healthy controls were examined, then the correlations between CSF1 and eosinophilic indicators were analyzed. Subsequently, bronchial epithelial cells (BEAS‐2B) with CSF1 overexpression and knockdown were constructed to investigate the potential molecular mechanism of CSF1. Finally, the effect of CSF1R inhibitor on STAT1 was investigated. Results The expression of CSF1 was significantly increased in patients with asthma compared to healthy controls, especially in patients with severe and eosinophilic asthma. Upregulated CSF1 positively correlated with airway‐increased eosinophil inflammation. In vitro, cytokines interleukin 13 (IL‐13) and IL‐33 can stimulate the upregulation of CSF1 expression. CSF1 overexpression enhanced p‐CSF1R/CSF1R and p‐STAT1/STAT1 expression, while knockdown CSF1 using anti‐CSF1 siRNAs decreased p‐CSF1R/CSF1R and p‐STAT1/STAT1 expression. Furthermore, the inhibitor of CSF1R significantly decreased p‐STAT1/STAT1 expression. Conclusions Sputum CSF1 may be involved in asthmatic airway eosinophil inflammation by interacting with CSF1R and further activating the STAT1 signaling. Interfering this potential pathway could serve as an anti‐inflammatory therapy for asthma. |
| format | Article |
| id | doaj-art-4f89c4beea2d41ab9cc17b2913516383 |
| institution | OA Journals |
| issn | 2050-4527 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Wiley |
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| series | Immunity, Inflammation and Disease |
| spelling | doaj-art-4f89c4beea2d41ab9cc17b29135163832025-08-20T02:09:27ZengWileyImmunity, Inflammation and Disease2050-45272023-05-01115n/an/a10.1002/iid3.847Increased expression of CSF1 in patients with eosinophilic asthmaLijuan Du0Lu Tang1Lisha Xiao2Kun Tang3Zhimin Zeng4Yuxia Liang5Yubiao Guo6Department of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Sun Yat‐Sen University Guangzhou Guangdong ChinaDepartment of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Sun Yat‐Sen University Guangzhou Guangdong ChinaDepartment of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Sun Yat‐Sen University Guangzhou Guangdong ChinaDepartment of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Sun Yat‐Sen University Guangzhou Guangdong ChinaDepartment of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Sun Yat‐Sen University Guangzhou Guangdong ChinaDepartment of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Sun Yat‐Sen University Guangzhou Guangdong ChinaDepartment of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Sun Yat‐Sen University Guangzhou Guangdong ChinaAbstract Background The link between colony‐stimulating factor 1 (CSF1) and asthma was reported recently. However, the role and mechanism of CSF1 in asthma remain poorly understood. In this study, we aimed to explore the expression and its potential mechanism of CSF1 in asthma. Methods CSF1 expression in the airway samples from asthmatics and healthy controls were examined, then the correlations between CSF1 and eosinophilic indicators were analyzed. Subsequently, bronchial epithelial cells (BEAS‐2B) with CSF1 overexpression and knockdown were constructed to investigate the potential molecular mechanism of CSF1. Finally, the effect of CSF1R inhibitor on STAT1 was investigated. Results The expression of CSF1 was significantly increased in patients with asthma compared to healthy controls, especially in patients with severe and eosinophilic asthma. Upregulated CSF1 positively correlated with airway‐increased eosinophil inflammation. In vitro, cytokines interleukin 13 (IL‐13) and IL‐33 can stimulate the upregulation of CSF1 expression. CSF1 overexpression enhanced p‐CSF1R/CSF1R and p‐STAT1/STAT1 expression, while knockdown CSF1 using anti‐CSF1 siRNAs decreased p‐CSF1R/CSF1R and p‐STAT1/STAT1 expression. Furthermore, the inhibitor of CSF1R significantly decreased p‐STAT1/STAT1 expression. Conclusions Sputum CSF1 may be involved in asthmatic airway eosinophil inflammation by interacting with CSF1R and further activating the STAT1 signaling. Interfering this potential pathway could serve as an anti‐inflammatory therapy for asthma.https://doi.org/10.1002/iid3.847airway eosinophil inflammationasthmaCSF1‐CSF1RSTAT1 |
| spellingShingle | Lijuan Du Lu Tang Lisha Xiao Kun Tang Zhimin Zeng Yuxia Liang Yubiao Guo Increased expression of CSF1 in patients with eosinophilic asthma Immunity, Inflammation and Disease airway eosinophil inflammation asthma CSF1‐CSF1R STAT1 |
| title | Increased expression of CSF1 in patients with eosinophilic asthma |
| title_full | Increased expression of CSF1 in patients with eosinophilic asthma |
| title_fullStr | Increased expression of CSF1 in patients with eosinophilic asthma |
| title_full_unstemmed | Increased expression of CSF1 in patients with eosinophilic asthma |
| title_short | Increased expression of CSF1 in patients with eosinophilic asthma |
| title_sort | increased expression of csf1 in patients with eosinophilic asthma |
| topic | airway eosinophil inflammation asthma CSF1‐CSF1R STAT1 |
| url | https://doi.org/10.1002/iid3.847 |
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