Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis.
<h4>Background</h4>Inflammatory Bowel Disease (IBD) is characterized by overt inflammation of the intestine and is typically accompanied by symptoms of bloody diarrhea, abdominal pain and cramping. The Colonic Migrating Motor Complex (CMMC) directs the movement of colonic luminal content...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199394&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850079061628420096 |
|---|---|
| author | Ben R Hofma Hannah R Wardill Chris Mavrangelos Melissa A Campaniello David Dimasi Joanne M Bowen Scott D Smid Claudine S Bonder Elizabeth A Beckett Patrick A Hughes |
| author_facet | Ben R Hofma Hannah R Wardill Chris Mavrangelos Melissa A Campaniello David Dimasi Joanne M Bowen Scott D Smid Claudine S Bonder Elizabeth A Beckett Patrick A Hughes |
| author_sort | Ben R Hofma |
| collection | DOAJ |
| description | <h4>Background</h4>Inflammatory Bowel Disease (IBD) is characterized by overt inflammation of the intestine and is typically accompanied by symptoms of bloody diarrhea, abdominal pain and cramping. The Colonic Migrating Motor Complex (CMMC) directs the movement of colonic luminal contents over long distances. The tri-nitrobenzene sulphonic acid (TNBS) model of colitis causes inflammatory damage to enteric nerves, however it remains to be determined whether these changes translate to functional outcomes in CMMC activity. We aimed to visualize innate immune cell infiltration into the colon using two-photon laser scanning intra-vital microscopy, and to determine whether CMMC activity is altered in the tri-nitro benzene sulphonic (TNBS) model of colitis.<h4>Methods</h4>Epithelial barrier permeability was compared between TNBS treated and healthy control mice in-vitro and in-vivo. Innate immune activation was determined by ELISA, flow cytometry and by 2-photon intravital microscopy. The effects of TNBS treatment and IL-1β on CMMC function were determined using a specialized organ bath.<h4>Results</h4>TNBS colitis increased epithelial barrier permeability in-vitro and in-vivo. Colonic IL-1β concentrations, colonic and systemic CD11b+ cell infiltration, and the number of migrating CD11b+ cells on colonic blood vessels were all increased in TNBS treated mice relative to controls. CMMC frequency and amplitude were inhibited in the distal and mid colon of TNBS treated mice. CMMC activity was not altered by superfusion with IL-1β.<h4>Conclusions</h4>TNBS colitis damages the epithelial barrier and increases innate immune cell activation in the colon and systemically. Innate cell migration into the colon is readily identifiable by two-photon intra-vital microscopy. CMMC are inhibited by inflammation, but this is not due to direct effects of IL-1β. |
| format | Article |
| id | doaj-art-4f84c26222984b7397a1905fd30a2a13 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4f84c26222984b7397a1905fd30a2a132025-08-20T02:45:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019939410.1371/journal.pone.0199394Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis.Ben R HofmaHannah R WardillChris MavrangelosMelissa A CampanielloDavid DimasiJoanne M BowenScott D SmidClaudine S BonderElizabeth A BeckettPatrick A Hughes<h4>Background</h4>Inflammatory Bowel Disease (IBD) is characterized by overt inflammation of the intestine and is typically accompanied by symptoms of bloody diarrhea, abdominal pain and cramping. The Colonic Migrating Motor Complex (CMMC) directs the movement of colonic luminal contents over long distances. The tri-nitrobenzene sulphonic acid (TNBS) model of colitis causes inflammatory damage to enteric nerves, however it remains to be determined whether these changes translate to functional outcomes in CMMC activity. We aimed to visualize innate immune cell infiltration into the colon using two-photon laser scanning intra-vital microscopy, and to determine whether CMMC activity is altered in the tri-nitro benzene sulphonic (TNBS) model of colitis.<h4>Methods</h4>Epithelial barrier permeability was compared between TNBS treated and healthy control mice in-vitro and in-vivo. Innate immune activation was determined by ELISA, flow cytometry and by 2-photon intravital microscopy. The effects of TNBS treatment and IL-1β on CMMC function were determined using a specialized organ bath.<h4>Results</h4>TNBS colitis increased epithelial barrier permeability in-vitro and in-vivo. Colonic IL-1β concentrations, colonic and systemic CD11b+ cell infiltration, and the number of migrating CD11b+ cells on colonic blood vessels were all increased in TNBS treated mice relative to controls. CMMC frequency and amplitude were inhibited in the distal and mid colon of TNBS treated mice. CMMC activity was not altered by superfusion with IL-1β.<h4>Conclusions</h4>TNBS colitis damages the epithelial barrier and increases innate immune cell activation in the colon and systemically. Innate cell migration into the colon is readily identifiable by two-photon intra-vital microscopy. CMMC are inhibited by inflammation, but this is not due to direct effects of IL-1β.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199394&type=printable |
| spellingShingle | Ben R Hofma Hannah R Wardill Chris Mavrangelos Melissa A Campaniello David Dimasi Joanne M Bowen Scott D Smid Claudine S Bonder Elizabeth A Beckett Patrick A Hughes Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis. PLoS ONE |
| title | Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis. |
| title_full | Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis. |
| title_fullStr | Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis. |
| title_full_unstemmed | Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis. |
| title_short | Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis. |
| title_sort | colonic migrating motor complexes are inhibited in acute tri nitro benzene sulphonic acid colitis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199394&type=printable |
| work_keys_str_mv | AT benrhofma colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT hannahrwardill colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT chrismavrangelos colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT melissaacampaniello colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT daviddimasi colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT joannembowen colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT scottdsmid colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT claudinesbonder colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT elizabethabeckett colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis AT patrickahughes colonicmigratingmotorcomplexesareinhibitedinacutetrinitrobenzenesulphonicacidcolitis |