In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications
β-Cell replacement through transplantation is the only curative treatment to establish a long-term stable euglycemia in diabetic patients. Owing to the shortage of donor tissue, attempts are being made to develop alternative sources of insulin-secreting cells. Stem cells differentiation and reprogra...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/5807876 |
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| author | Guoguang Niu John P. McQuilling Yu Zhou Emmanuel C. Opara Giuseppe Orlando Shay Soker |
| author_facet | Guoguang Niu John P. McQuilling Yu Zhou Emmanuel C. Opara Giuseppe Orlando Shay Soker |
| author_sort | Guoguang Niu |
| collection | DOAJ |
| description | β-Cell replacement through transplantation is the only curative treatment to establish a long-term stable euglycemia in diabetic patients. Owing to the shortage of donor tissue, attempts are being made to develop alternative sources of insulin-secreting cells. Stem cells differentiation and reprograming as well as isolating pancreatic progenitors from different sources are some examples; however, no approach has yet yielded a clinically relevant solution. Dissociated islet cells that are cultured in cell numbers by in vitro proliferation provide a promising platform for redifferentiation towards β-cells phenotype. In this study, we cultured islet-derived cells in vitro and examined the expression of β-cell genes during the proliferation. Islets were isolated from porcine pancreases and enzymatically digested to dissociate the component cells. The cells proliferated well in tissue culture plates and were subcultured for no more than 5 passages. Only 10% of insulin expression, as measured by PCR, was preserved in each passage. High glucose media enhanced insulin expression by about 4–18 fold, suggesting a glucose-dependent effect in the proliferated islet-derived cells. The islet-derived cells also expressed other pancreatic genes such as Pdx1, NeuroD, glucagon, and somatostatin. Taken together, these results indicate that pancreatic islet-derived cells, proliferated in vitro, retained the expression capacity for key pancreatic genes, thus suggesting that the cells may be redifferentiated into insulin-secreting β-like cells. |
| format | Article |
| id | doaj-art-4f81801565b9472ea4e1902883432387 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-4f81801565b9472ea4e19028834323872025-02-03T01:32:08ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/58078765807876In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy ApplicationsGuoguang Niu0John P. McQuilling1Yu Zhou2Emmanuel C. Opara3Giuseppe Orlando4Shay Soker5Wake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Medical Center Boulevard, Winston-Salem, NC 27157, USAWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Medical Center Boulevard, Winston-Salem, NC 27157, USAWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Medical Center Boulevard, Winston-Salem, NC 27157, USAWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Medical Center Boulevard, Winston-Salem, NC 27157, USAWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Medical Center Boulevard, Winston-Salem, NC 27157, USAWake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Medical Center Boulevard, Winston-Salem, NC 27157, USAβ-Cell replacement through transplantation is the only curative treatment to establish a long-term stable euglycemia in diabetic patients. Owing to the shortage of donor tissue, attempts are being made to develop alternative sources of insulin-secreting cells. Stem cells differentiation and reprograming as well as isolating pancreatic progenitors from different sources are some examples; however, no approach has yet yielded a clinically relevant solution. Dissociated islet cells that are cultured in cell numbers by in vitro proliferation provide a promising platform for redifferentiation towards β-cells phenotype. In this study, we cultured islet-derived cells in vitro and examined the expression of β-cell genes during the proliferation. Islets were isolated from porcine pancreases and enzymatically digested to dissociate the component cells. The cells proliferated well in tissue culture plates and were subcultured for no more than 5 passages. Only 10% of insulin expression, as measured by PCR, was preserved in each passage. High glucose media enhanced insulin expression by about 4–18 fold, suggesting a glucose-dependent effect in the proliferated islet-derived cells. The islet-derived cells also expressed other pancreatic genes such as Pdx1, NeuroD, glucagon, and somatostatin. Taken together, these results indicate that pancreatic islet-derived cells, proliferated in vitro, retained the expression capacity for key pancreatic genes, thus suggesting that the cells may be redifferentiated into insulin-secreting β-like cells.http://dx.doi.org/10.1155/2016/5807876 |
| spellingShingle | Guoguang Niu John P. McQuilling Yu Zhou Emmanuel C. Opara Giuseppe Orlando Shay Soker In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications Journal of Diabetes Research |
| title | In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications |
| title_full | In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications |
| title_fullStr | In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications |
| title_full_unstemmed | In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications |
| title_short | In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications |
| title_sort | in vitro proliferation of porcine pancreatic islet cells for β cell therapy applications |
| url | http://dx.doi.org/10.1155/2016/5807876 |
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