Plasmolipin deficiency is essential for HUVECs survival under hypoxic conditions
Abstract This study aims to explore the molecules that affect the survival of Human Umbilical Vein Endothelial Cells (HUVECs) under hypoxia and their mechanisms of action. In hypoxia, plasmolipin (PLLP) was identified through the screening of CRISPR/Cas9 and small guide RNA (sgRNA) library. Function...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2025-05-01
|
| Series: | Cell Death Discovery |
| Online Access: | https://doi.org/10.1038/s41420-025-02526-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849744674076491776 |
|---|---|
| author | Yanghua Li Weiling Man Xiang Li Xiaojie Wu Yumeng Cui Shiyun Chen Xianhong Li Yanli Lin Lihe Jiang Youliang Wang |
| author_facet | Yanghua Li Weiling Man Xiang Li Xiaojie Wu Yumeng Cui Shiyun Chen Xianhong Li Yanli Lin Lihe Jiang Youliang Wang |
| author_sort | Yanghua Li |
| collection | DOAJ |
| description | Abstract This study aims to explore the molecules that affect the survival of Human Umbilical Vein Endothelial Cells (HUVECs) under hypoxia and their mechanisms of action. In hypoxia, plasmolipin (PLLP) was identified through the screening of CRISPR/Cas9 and small guide RNA (sgRNA) library. Functionally, PLLP knockout led to increase cell proliferation, cellular metabolism, tight junction formation, angiogenesis ability, migration and invasion in hypoxic HUVECs. Furthermore, PLLP knockout countered the inhibitory effects of bevacizumab on HUVECs angiogenesis and cell survival in hypoxic conditions. PLLP knockout was found to modulate the survival of HUVECs in hypoxia by enhancing the phosphorylation of AKT and ERK1/2 proteins. In conclusion, inhibiting the expression of PLLP in HUVECs promotes cell survival and maintenance of cellular functions under hypoxic condition. PLLP plays a crucial role in regulating cell survival in hypoxia through the activation of AKT and ERK1/2 pathways. This study identifies novel molecules that affect HUVECs survival under hypoxic conditions and provides a new possibility for future studies on cell survival under hypoxic conditions. |
| format | Article |
| id | doaj-art-4f71a9520e694b61bf4b2a5e0d98c13f |
| institution | DOAJ |
| issn | 2058-7716 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death Discovery |
| spelling | doaj-art-4f71a9520e694b61bf4b2a5e0d98c13f2025-08-20T03:10:13ZengNature Publishing GroupCell Death Discovery2058-77162025-05-0111111410.1038/s41420-025-02526-5Plasmolipin deficiency is essential for HUVECs survival under hypoxic conditionsYanghua Li0Weiling Man1Xiang Li2Xiaojie Wu3Yumeng Cui4Shiyun Chen5Xianhong Li6Yanli Lin7Lihe Jiang8Youliang Wang9Medical College, Guangxi UniversityLaboratory of Advanced Biotechnology, Beijing Institute of BiotechnologyLaboratory of Advanced Biotechnology, Beijing Institute of BiotechnologyLaboratory of Advanced Biotechnology, Beijing Institute of BiotechnologyLaboratory of Advanced Biotechnology, Beijing Institute of BiotechnologyLaboratory of Advanced Biotechnology, Beijing Institute of BiotechnologyLaboratory of Advanced Biotechnology, Beijing Institute of BiotechnologyLaboratory of Advanced Biotechnology, Beijing Institute of BiotechnologyMedical College, Guangxi UniversityLaboratory of Advanced Biotechnology, Beijing Institute of BiotechnologyAbstract This study aims to explore the molecules that affect the survival of Human Umbilical Vein Endothelial Cells (HUVECs) under hypoxia and their mechanisms of action. In hypoxia, plasmolipin (PLLP) was identified through the screening of CRISPR/Cas9 and small guide RNA (sgRNA) library. Functionally, PLLP knockout led to increase cell proliferation, cellular metabolism, tight junction formation, angiogenesis ability, migration and invasion in hypoxic HUVECs. Furthermore, PLLP knockout countered the inhibitory effects of bevacizumab on HUVECs angiogenesis and cell survival in hypoxic conditions. PLLP knockout was found to modulate the survival of HUVECs in hypoxia by enhancing the phosphorylation of AKT and ERK1/2 proteins. In conclusion, inhibiting the expression of PLLP in HUVECs promotes cell survival and maintenance of cellular functions under hypoxic condition. PLLP plays a crucial role in regulating cell survival in hypoxia through the activation of AKT and ERK1/2 pathways. This study identifies novel molecules that affect HUVECs survival under hypoxic conditions and provides a new possibility for future studies on cell survival under hypoxic conditions.https://doi.org/10.1038/s41420-025-02526-5 |
| spellingShingle | Yanghua Li Weiling Man Xiang Li Xiaojie Wu Yumeng Cui Shiyun Chen Xianhong Li Yanli Lin Lihe Jiang Youliang Wang Plasmolipin deficiency is essential for HUVECs survival under hypoxic conditions Cell Death Discovery |
| title | Plasmolipin deficiency is essential for HUVECs survival under hypoxic conditions |
| title_full | Plasmolipin deficiency is essential for HUVECs survival under hypoxic conditions |
| title_fullStr | Plasmolipin deficiency is essential for HUVECs survival under hypoxic conditions |
| title_full_unstemmed | Plasmolipin deficiency is essential for HUVECs survival under hypoxic conditions |
| title_short | Plasmolipin deficiency is essential for HUVECs survival under hypoxic conditions |
| title_sort | plasmolipin deficiency is essential for huvecs survival under hypoxic conditions |
| url | https://doi.org/10.1038/s41420-025-02526-5 |
| work_keys_str_mv | AT yanghuali plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT weilingman plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT xiangli plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT xiaojiewu plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT yumengcui plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT shiyunchen plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT xianhongli plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT yanlilin plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT lihejiang plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions AT youliangwang plasmolipindeficiencyisessentialforhuvecssurvivalunderhypoxicconditions |