Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro study
The research project focuses on conducting an in vitro comparison between sodium alginate and chitosan electrospun nanofibers for moist wound management. These fabricated nanofibers were incorporated with levofloxacin (Lev) as a model drug to investigate their release behavior. The study included a...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-11-01
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| Series: | Nanocomposites |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20550324.2024.2362534 |
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| author | Sharjeel Abid Ling Wang Md. Kaiser Haider Gopiraman Mayakrishnan Rajamani Lakshminarayanan Kyu Oh Kim Azeem Ullah Ick Soo Kim |
| author_facet | Sharjeel Abid Ling Wang Md. Kaiser Haider Gopiraman Mayakrishnan Rajamani Lakshminarayanan Kyu Oh Kim Azeem Ullah Ick Soo Kim |
| author_sort | Sharjeel Abid |
| collection | DOAJ |
| description | The research project focuses on conducting an in vitro comparison between sodium alginate and chitosan electrospun nanofibers for moist wound management. These fabricated nanofibers were incorporated with levofloxacin (Lev) as a model drug to investigate their release behavior. The study included a comprehensive examination of the physicochemical, thermal, antibacterial, and cytocompatibility properties of these nanofibers. Both sodium alginate (SA-L) and chitosan (CS-L) nanofibers were successfully produced with a homogeneous and defect-free structure. XRD and FTIR analyses were conducted to validate the incorporation of Lev in the nanofibers. In terms of liquid absorption capacity, it was observed that sodium alginate nanofibers outperformed chitosan nanofibers, exhibiting absorption capacities of 5.9, 5.88, and 7.1 g/g for PBS, solution A, and DI, respectively, for SA-L, compared to 5.27, 4.42, and 5.3 g/g for CS-L nanofibers. This superior absorption ability in SA-L nanofibers may be attributed to the inherent gel-forming properties of alginate in aqueous environments. Furthermore, the release kinetics indicated that SA-L nanofibers exhibited faster drug release compared to CS-L nanofibers, which could be attributed to alginate’s natural gel-forming tendency in aqueous mediums. Both SA-L and CS-L nanofibers demonstrated remarkable efficacy against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa), with CS-L nanofibers displaying larger inhibition zones in agar diffusion tests due to chitosan’s inherent antibacterial properties. Moreover, cytocompatibility assessments using the HaCat cell line revealed that the prepared nanofibers were biocompatible and non-toxic. Interestingly, CS-L nanofibers exhibited superior cell proliferation when compared to SA-L nanofibers, potentially attributed to the inherent positively charged amine groups which enhance proliferation and migration of negatively charged keratinocytes. |
| format | Article |
| id | doaj-art-4f6df2b2f1264ac2b48a47b13612c9b3 |
| institution | OA Journals |
| issn | 2055-0324 2055-0332 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Nanocomposites |
| spelling | doaj-art-4f6df2b2f1264ac2b48a47b13612c9b32025-08-20T02:26:56ZengTaylor & Francis GroupNanocomposites2055-03242055-03322024-11-0110124225510.1080/20550324.2024.2362534Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro studySharjeel Abid0Ling Wang1Md. Kaiser Haider2Gopiraman Mayakrishnan3Rajamani Lakshminarayanan4Kyu Oh Kim5Azeem Ullah6Ick Soo Kim7Department of Textile Engineering, School of Engineering and Technology, National Textile University (NTU), PakistanNano Fusion Technology Research Group, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Ueda, Nagano, JapanNano Fusion Technology Research Group, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Ueda, Nagano, JapanNano Fusion Technology Research Group, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Ueda, Nagano, JapanDepartment of Pharmacy, National University of Singapore, Singapore, SingaporeDepartment of Fiber System Engineering, Dankook University, Yongin-si, Gyeonggi-do, Republic of KoreaNano Fusion Technology Research Group, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Ueda, Nagano, JapanNano Fusion Technology Research Group, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Ueda, Nagano, JapanThe research project focuses on conducting an in vitro comparison between sodium alginate and chitosan electrospun nanofibers for moist wound management. These fabricated nanofibers were incorporated with levofloxacin (Lev) as a model drug to investigate their release behavior. The study included a comprehensive examination of the physicochemical, thermal, antibacterial, and cytocompatibility properties of these nanofibers. Both sodium alginate (SA-L) and chitosan (CS-L) nanofibers were successfully produced with a homogeneous and defect-free structure. XRD and FTIR analyses were conducted to validate the incorporation of Lev in the nanofibers. In terms of liquid absorption capacity, it was observed that sodium alginate nanofibers outperformed chitosan nanofibers, exhibiting absorption capacities of 5.9, 5.88, and 7.1 g/g for PBS, solution A, and DI, respectively, for SA-L, compared to 5.27, 4.42, and 5.3 g/g for CS-L nanofibers. This superior absorption ability in SA-L nanofibers may be attributed to the inherent gel-forming properties of alginate in aqueous environments. Furthermore, the release kinetics indicated that SA-L nanofibers exhibited faster drug release compared to CS-L nanofibers, which could be attributed to alginate’s natural gel-forming tendency in aqueous mediums. Both SA-L and CS-L nanofibers demonstrated remarkable efficacy against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa), with CS-L nanofibers displaying larger inhibition zones in agar diffusion tests due to chitosan’s inherent antibacterial properties. Moreover, cytocompatibility assessments using the HaCat cell line revealed that the prepared nanofibers were biocompatible and non-toxic. Interestingly, CS-L nanofibers exhibited superior cell proliferation when compared to SA-L nanofibers, potentially attributed to the inherent positively charged amine groups which enhance proliferation and migration of negatively charged keratinocytes.https://www.tandfonline.com/doi/10.1080/20550324.2024.2362534Biocompatibilitychitosanelectrospinningmoist wound managementnanofiberssodium alginate |
| spellingShingle | Sharjeel Abid Ling Wang Md. Kaiser Haider Gopiraman Mayakrishnan Rajamani Lakshminarayanan Kyu Oh Kim Azeem Ullah Ick Soo Kim Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro study Nanocomposites Biocompatibility chitosan electrospinning moist wound management nanofibers sodium alginate |
| title | Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro study |
| title_full | Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro study |
| title_fullStr | Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro study |
| title_full_unstemmed | Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro study |
| title_short | Investigating alginate and chitosan electrospun nanofibers as a potential wound dressing: an in vitro study |
| title_sort | investigating alginate and chitosan electrospun nanofibers as a potential wound dressing an in vitro study |
| topic | Biocompatibility chitosan electrospinning moist wound management nanofibers sodium alginate |
| url | https://www.tandfonline.com/doi/10.1080/20550324.2024.2362534 |
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