Salivary Glands Proteins Expression of Anopheles dirus A Fed on Plasmodium vivax- and Plasmodium falciparum-Infected Human Blood
Mosquitoes are able to adapt to feed on blood by the salivary glands which created a protein that works against the haemostasis process. This study aims to investigate the salivary glands proteins expression of 50 adult female An. dirus A mosquitoes, a main vector of malaria in Thailand, each group...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2013/535267 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558524405645312 |
|---|---|
| author | Saowanee Cotama Paron Dekumyoy Yudthana Samung Usa Lek-Uthai |
| author_facet | Saowanee Cotama Paron Dekumyoy Yudthana Samung Usa Lek-Uthai |
| author_sort | Saowanee Cotama |
| collection | DOAJ |
| description | Mosquitoes are able to adapt to feed on blood by the salivary glands which created a protein that works against the haemostasis process. This study aims to investigate the salivary glands proteins expression of 50 adult female An. dirus A mosquitoes, a main vector of malaria in Thailand, each group with an age of 5 days which were artificial membrane fed on sugar, normal blood, blood infected with P. vivax, and blood infected with P. falciparum. Then mosquito salivary gland proteins were analyzed by SDS-PAGE on days 0, 1, 2, 3, and 4 after feeding. The findings revealed that the major salivary glands proteins had molecular weights of 62, 58, 43, 36, 33, 30, and 18 kDa. One protein band of approximately 13 kDa was found in normal blood and blood infected with P. vivax fed on day 0. A stronger protein band, 65 kDa, was expressed from the salivary glands of mosquitoes fed with P. vivax- or P. falciparum-infected blood on only day 0, but none on days 1 to 4. The study shows that salivary glands proteins expression of An. dirus may affect the malaria parasite life cycle and the ability of mosquitoes to transmit malaria parasites in post-24-hour disappearance observation. |
| format | Article |
| id | doaj-art-4f6c7e1cd1ba4eb4bea04801bf31c2a4 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-4f6c7e1cd1ba4eb4bea04801bf31c2a42025-02-03T01:32:09ZengWileyJournal of Parasitology Research2090-00232090-00312013-01-01201310.1155/2013/535267535267Salivary Glands Proteins Expression of Anopheles dirus A Fed on Plasmodium vivax- and Plasmodium falciparum-Infected Human BloodSaowanee Cotama0Paron Dekumyoy1Yudthana Samung2Usa Lek-Uthai3Program in Infectious Diseases and Epidemiology, Faculty of Graduate Studies, Mahidol University, Nakornpathom 73170, ThailandDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Rajvithi Road, Bangkok 10400, ThailandDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Rajvithi Road, Bangkok 10400, ThailandDepartment of Parasitology and Entomology, Faculty of Public Health, Mahidol University, Rajvithi Road, Bangkok 10400, ThailandMosquitoes are able to adapt to feed on blood by the salivary glands which created a protein that works against the haemostasis process. This study aims to investigate the salivary glands proteins expression of 50 adult female An. dirus A mosquitoes, a main vector of malaria in Thailand, each group with an age of 5 days which were artificial membrane fed on sugar, normal blood, blood infected with P. vivax, and blood infected with P. falciparum. Then mosquito salivary gland proteins were analyzed by SDS-PAGE on days 0, 1, 2, 3, and 4 after feeding. The findings revealed that the major salivary glands proteins had molecular weights of 62, 58, 43, 36, 33, 30, and 18 kDa. One protein band of approximately 13 kDa was found in normal blood and blood infected with P. vivax fed on day 0. A stronger protein band, 65 kDa, was expressed from the salivary glands of mosquitoes fed with P. vivax- or P. falciparum-infected blood on only day 0, but none on days 1 to 4. The study shows that salivary glands proteins expression of An. dirus may affect the malaria parasite life cycle and the ability of mosquitoes to transmit malaria parasites in post-24-hour disappearance observation.http://dx.doi.org/10.1155/2013/535267 |
| spellingShingle | Saowanee Cotama Paron Dekumyoy Yudthana Samung Usa Lek-Uthai Salivary Glands Proteins Expression of Anopheles dirus A Fed on Plasmodium vivax- and Plasmodium falciparum-Infected Human Blood Journal of Parasitology Research |
| title | Salivary Glands Proteins Expression of Anopheles dirus A Fed on Plasmodium vivax- and Plasmodium falciparum-Infected Human Blood |
| title_full | Salivary Glands Proteins Expression of Anopheles dirus A Fed on Plasmodium vivax- and Plasmodium falciparum-Infected Human Blood |
| title_fullStr | Salivary Glands Proteins Expression of Anopheles dirus A Fed on Plasmodium vivax- and Plasmodium falciparum-Infected Human Blood |
| title_full_unstemmed | Salivary Glands Proteins Expression of Anopheles dirus A Fed on Plasmodium vivax- and Plasmodium falciparum-Infected Human Blood |
| title_short | Salivary Glands Proteins Expression of Anopheles dirus A Fed on Plasmodium vivax- and Plasmodium falciparum-Infected Human Blood |
| title_sort | salivary glands proteins expression of anopheles dirus a fed on plasmodium vivax and plasmodium falciparum infected human blood |
| url | http://dx.doi.org/10.1155/2013/535267 |
| work_keys_str_mv | AT saowaneecotama salivaryglandsproteinsexpressionofanophelesdirusafedonplasmodiumvivaxandplasmodiumfalciparuminfectedhumanblood AT parondekumyoy salivaryglandsproteinsexpressionofanophelesdirusafedonplasmodiumvivaxandplasmodiumfalciparuminfectedhumanblood AT yudthanasamung salivaryglandsproteinsexpressionofanophelesdirusafedonplasmodiumvivaxandplasmodiumfalciparuminfectedhumanblood AT usalekuthai salivaryglandsproteinsexpressionofanophelesdirusafedonplasmodiumvivaxandplasmodiumfalciparuminfectedhumanblood |