Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in Nanotechnologies
Multiplexed biosensing methods for simultaneously detecting multiple biomolecules are important for investigating biological mechanisms associated with physiological processes, developing applications in life sciences, and conducting medical tests. The development of biosensors, especially those adv...
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MDPI AG
2024-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/14/24/2014 |
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| author | Shangjie Zou Guangdun Peng Zhiqiang Ma |
| author_facet | Shangjie Zou Guangdun Peng Zhiqiang Ma |
| author_sort | Shangjie Zou |
| collection | DOAJ |
| description | Multiplexed biosensing methods for simultaneously detecting multiple biomolecules are important for investigating biological mechanisms associated with physiological processes, developing applications in life sciences, and conducting medical tests. The development of biosensors, especially those advanced biosensors with multiplexing potentials, strongly depends on advancements in nanotechnologies, including the nano-coating of thin films, micro–nano 3D structures, and nanotags for signal generation. Surface functionalization is a critical process for biosensing applications, one which enables the immobilization of biological probes or other structures that assist in the capturing of biomolecules. During this functionalizing process, nanomaterials can either be the objects of surface modification or the materials used to modify other base surfaces. These surface-functionalizing strategies, involving the coordination of sensor structures and materials, as well as the associated modifying methods, are largely determinative in the performance of biosensing applications. This review introduces the current studies on biosensors with multiplexing potentials and focuses specifically on the roles of nanomaterials in the design and functionalization of these biosensors. A detailed description of the paradigms used for method selection has been set forth to assist understanding and accelerate the application of novel nanotechnologies in the development of biosensors. |
| format | Article |
| id | doaj-art-4f60ee070ca04eef9f79158109cc2bdd |
| institution | DOAJ |
| issn | 2079-4991 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj-art-4f60ee070ca04eef9f79158109cc2bdd2025-08-20T02:43:20ZengMDPI AGNanomaterials2079-49912024-12-011424201410.3390/nano14242014Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in NanotechnologiesShangjie Zou0Guangdun Peng1Zhiqiang Ma2Center for Cell Lineage Technology and Engineering, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, GIBH-CUHK Joint Research Laboratory on Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, China-New Zealand Belt and Road Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaCenter for Cell Lineage Technology and Engineering, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, GIBH-CUHK Joint Research Laboratory on Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, China-New Zealand Belt and Road Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaDepartment of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong 999077, ChinaMultiplexed biosensing methods for simultaneously detecting multiple biomolecules are important for investigating biological mechanisms associated with physiological processes, developing applications in life sciences, and conducting medical tests. The development of biosensors, especially those advanced biosensors with multiplexing potentials, strongly depends on advancements in nanotechnologies, including the nano-coating of thin films, micro–nano 3D structures, and nanotags for signal generation. Surface functionalization is a critical process for biosensing applications, one which enables the immobilization of biological probes or other structures that assist in the capturing of biomolecules. During this functionalizing process, nanomaterials can either be the objects of surface modification or the materials used to modify other base surfaces. These surface-functionalizing strategies, involving the coordination of sensor structures and materials, as well as the associated modifying methods, are largely determinative in the performance of biosensing applications. This review introduces the current studies on biosensors with multiplexing potentials and focuses specifically on the roles of nanomaterials in the design and functionalization of these biosensors. A detailed description of the paradigms used for method selection has been set forth to assist understanding and accelerate the application of novel nanotechnologies in the development of biosensors.https://www.mdpi.com/2079-4991/14/24/2014surface functionalizationnanomaterialsbiosensormultiplexingsensor performance |
| spellingShingle | Shangjie Zou Guangdun Peng Zhiqiang Ma Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in Nanotechnologies Nanomaterials surface functionalization nanomaterials biosensor multiplexing sensor performance |
| title | Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in Nanotechnologies |
| title_full | Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in Nanotechnologies |
| title_fullStr | Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in Nanotechnologies |
| title_full_unstemmed | Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in Nanotechnologies |
| title_short | Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in Nanotechnologies |
| title_sort | surface functionalizing strategies for multiplexed molecular biosensing developments powered by advancements in nanotechnologies |
| topic | surface functionalization nanomaterials biosensor multiplexing sensor performance |
| url | https://www.mdpi.com/2079-4991/14/24/2014 |
| work_keys_str_mv | AT shangjiezou surfacefunctionalizingstrategiesformultiplexedmolecularbiosensingdevelopmentspoweredbyadvancementsinnanotechnologies AT guangdunpeng surfacefunctionalizingstrategiesformultiplexedmolecularbiosensingdevelopmentspoweredbyadvancementsinnanotechnologies AT zhiqiangma surfacefunctionalizingstrategiesformultiplexedmolecularbiosensingdevelopmentspoweredbyadvancementsinnanotechnologies |