Mechanisms Involved in Soil–Plant Interactions in Response to Poultry Manure and Phytase Enzyme Compared to Inorganic Phosphorus Fertilizers
While soil responses to organic and inorganic phosphorus (P) fertilizers have been widely studied, plant physiological and molecular responses remain insufficiently characterized. Such an understanding is necessary to develop sustainable P fertilization strategies that enhance plant performance in s...
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MDPI AG
2025-03-01
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| Series: | Agronomy |
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| author | Patricia Poblete-Grant Leyla Parra-Almuna Sofía Pontigo Cornelia Rumpel María de La Luz Mora Paula Cartes |
| author_facet | Patricia Poblete-Grant Leyla Parra-Almuna Sofía Pontigo Cornelia Rumpel María de La Luz Mora Paula Cartes |
| author_sort | Patricia Poblete-Grant |
| collection | DOAJ |
| description | While soil responses to organic and inorganic phosphorus (P) fertilizers have been widely studied, plant physiological and molecular responses remain insufficiently characterized. Such an understanding is necessary to develop sustainable P fertilization strategies that enhance plant performance in soils with P limitations. This study investigated the impact of poultry manure (PM) and its combination with phytase enzyme on molecular plant responses involved in P use efficiency (PUE) of ryegrass plants growing on a P-deficient Andisol. A greenhouse experiment under controlled conditions was performed to evaluate soil properties, plant biomass, P uptake, plant performance, and the expression of P transporters under the following P treatments: P deficiency (PD), mineral fertilizers (F), PM alone, and PM combined with phytase. The combination of PM and phytase enhanced soil P availability by 60% and increased soil P enzyme activities 2.6-fold, facilitating the mineralization of organic P. This resulted in a 63% increase in shoot P concentration and a 35% enhancement in shoot biomass. Additionally, oxidative stress markers decreased, with lipid peroxidation in roots reduced up to five-fold, while antioxidant activity increased 1.6-fold. Molecular analysis revealed that the expression of the P transporter gene <i>LpPHT1;4</i> was upregulated 9.3-fold, indicating an improved capacity for P acquisition and utilization. These findings suggest that phytase-mediated hydrolysis of organic P and the activation of plant P transporters are key mechanisms driving enhanced P uptake and efficiency in P-deficient soils. |
| format | Article |
| id | doaj-art-4f600ccf957b4605bbdc58a8291cbd7b |
| institution | OA Journals |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-4f600ccf957b4605bbdc58a8291cbd7b2025-08-20T02:11:21ZengMDPI AGAgronomy2073-43952025-03-0115366010.3390/agronomy15030660Mechanisms Involved in Soil–Plant Interactions in Response to Poultry Manure and Phytase Enzyme Compared to Inorganic Phosphorus FertilizersPatricia Poblete-Grant0Leyla Parra-Almuna1Sofía Pontigo2Cornelia Rumpel3María de La Luz Mora4Paula Cartes5Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Casilla 54-D, Temuco 4780000, ChileCenter of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Casilla 54-D, Temuco 4780000, ChileCenter of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Casilla 54-D, Temuco 4780000, ChileCNRS, Institute for Ecology and Environmental Sciences IEES (UMR 7618, CNRS-UPMC-UPEC-IRD-INRA), 78026 Paris, FranceCenter of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Casilla 54-D, Temuco 4780000, ChileCenter of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Casilla 54-D, Temuco 4780000, ChileWhile soil responses to organic and inorganic phosphorus (P) fertilizers have been widely studied, plant physiological and molecular responses remain insufficiently characterized. Such an understanding is necessary to develop sustainable P fertilization strategies that enhance plant performance in soils with P limitations. This study investigated the impact of poultry manure (PM) and its combination with phytase enzyme on molecular plant responses involved in P use efficiency (PUE) of ryegrass plants growing on a P-deficient Andisol. A greenhouse experiment under controlled conditions was performed to evaluate soil properties, plant biomass, P uptake, plant performance, and the expression of P transporters under the following P treatments: P deficiency (PD), mineral fertilizers (F), PM alone, and PM combined with phytase. The combination of PM and phytase enhanced soil P availability by 60% and increased soil P enzyme activities 2.6-fold, facilitating the mineralization of organic P. This resulted in a 63% increase in shoot P concentration and a 35% enhancement in shoot biomass. Additionally, oxidative stress markers decreased, with lipid peroxidation in roots reduced up to five-fold, while antioxidant activity increased 1.6-fold. Molecular analysis revealed that the expression of the P transporter gene <i>LpPHT1;4</i> was upregulated 9.3-fold, indicating an improved capacity for P acquisition and utilization. These findings suggest that phytase-mediated hydrolysis of organic P and the activation of plant P transporters are key mechanisms driving enhanced P uptake and efficiency in P-deficient soils.https://www.mdpi.com/2073-4395/15/3/660phosphorus uptakepoultry manuresoil phosphorus availabilityphosphorus transporterssustainable fertilization |
| spellingShingle | Patricia Poblete-Grant Leyla Parra-Almuna Sofía Pontigo Cornelia Rumpel María de La Luz Mora Paula Cartes Mechanisms Involved in Soil–Plant Interactions in Response to Poultry Manure and Phytase Enzyme Compared to Inorganic Phosphorus Fertilizers Agronomy phosphorus uptake poultry manure soil phosphorus availability phosphorus transporters sustainable fertilization |
| title | Mechanisms Involved in Soil–Plant Interactions in Response to Poultry Manure and Phytase Enzyme Compared to Inorganic Phosphorus Fertilizers |
| title_full | Mechanisms Involved in Soil–Plant Interactions in Response to Poultry Manure and Phytase Enzyme Compared to Inorganic Phosphorus Fertilizers |
| title_fullStr | Mechanisms Involved in Soil–Plant Interactions in Response to Poultry Manure and Phytase Enzyme Compared to Inorganic Phosphorus Fertilizers |
| title_full_unstemmed | Mechanisms Involved in Soil–Plant Interactions in Response to Poultry Manure and Phytase Enzyme Compared to Inorganic Phosphorus Fertilizers |
| title_short | Mechanisms Involved in Soil–Plant Interactions in Response to Poultry Manure and Phytase Enzyme Compared to Inorganic Phosphorus Fertilizers |
| title_sort | mechanisms involved in soil plant interactions in response to poultry manure and phytase enzyme compared to inorganic phosphorus fertilizers |
| topic | phosphorus uptake poultry manure soil phosphorus availability phosphorus transporters sustainable fertilization |
| url | https://www.mdpi.com/2073-4395/15/3/660 |
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