Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselae
Abstract The Bartonella genus of bacteria encompasses ubiquitous species, some of which are pathogenic in humans and animals. Bartonella henselae, the causative agent of Cat Scratch disease, is responsible for a large portion of human Bartonella infections. These bacteria can grow outside of cells,...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07535-9 |
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| author | Shiva Kumar Goud Gadila John R. Caskey Edward B. Breitschwerdt Ricardo G. Maggi Monica E. Embers |
| author_facet | Shiva Kumar Goud Gadila John R. Caskey Edward B. Breitschwerdt Ricardo G. Maggi Monica E. Embers |
| author_sort | Shiva Kumar Goud Gadila |
| collection | DOAJ |
| description | Abstract The Bartonella genus of bacteria encompasses ubiquitous species, some of which are pathogenic in humans and animals. Bartonella henselae, the causative agent of Cat Scratch disease, is responsible for a large portion of human Bartonella infections. These bacteria can grow outside of cells, replicate in erythrocytes and invade endothelial and monocytic cells. We have previously reported reduced antibiotic susceptibility of intracellular Bartonella. In this study we performed comparative transcriptomic analyses between the extracellular and intracellular B. henselae phenotypes. Overall, specific genes involved in invasion, virulence, extracellular adhesion of type 4 secretion system were downregulated following intracellular invasion of B. henselae. Downregulation included BadA, a well-characterized adhesin molecule, of critical importance for cell invasion. These studies demonstrate the ability to purify Bartonella RNA from infected cells and offer a repository of gene expression data for future research. The development of novel therapeutics will benefit from the ability to determine target expression by Bartonella in relevant microenvironments. |
| format | Article |
| id | doaj-art-4f5e7f5b73a24d178d753cc013063eac |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-4f5e7f5b73a24d178d753cc013063eac2025-02-02T12:37:08ZengNature PortfolioCommunications Biology2399-36422025-01-018111610.1038/s42003-025-07535-9Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselaeShiva Kumar Goud Gadila0John R. Caskey1Edward B. Breitschwerdt2Ricardo G. Maggi3Monica E. Embers4Division of Immunology, Tulane National Primate Research Center, Tulane UniversityDepartment of Medicine, Clinical Science Center, University of Wisconsin School of Medicine and Public HealthIntracellular Pathogens Research Laboratory, Department of Clinical Sciences, Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State UniversityIntracellular Pathogens Research Laboratory, Department of Clinical Sciences, Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State UniversityDivision of Immunology, Tulane National Primate Research Center, Tulane UniversityAbstract The Bartonella genus of bacteria encompasses ubiquitous species, some of which are pathogenic in humans and animals. Bartonella henselae, the causative agent of Cat Scratch disease, is responsible for a large portion of human Bartonella infections. These bacteria can grow outside of cells, replicate in erythrocytes and invade endothelial and monocytic cells. We have previously reported reduced antibiotic susceptibility of intracellular Bartonella. In this study we performed comparative transcriptomic analyses between the extracellular and intracellular B. henselae phenotypes. Overall, specific genes involved in invasion, virulence, extracellular adhesion of type 4 secretion system were downregulated following intracellular invasion of B. henselae. Downregulation included BadA, a well-characterized adhesin molecule, of critical importance for cell invasion. These studies demonstrate the ability to purify Bartonella RNA from infected cells and offer a repository of gene expression data for future research. The development of novel therapeutics will benefit from the ability to determine target expression by Bartonella in relevant microenvironments.https://doi.org/10.1038/s42003-025-07535-9 |
| spellingShingle | Shiva Kumar Goud Gadila John R. Caskey Edward B. Breitschwerdt Ricardo G. Maggi Monica E. Embers Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselae Communications Biology |
| title | Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselae |
| title_full | Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselae |
| title_fullStr | Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselae |
| title_full_unstemmed | Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselae |
| title_short | Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselae |
| title_sort | comparison of transcriptomic profiles between intracellular and extracellular bartonella henselae |
| url | https://doi.org/10.1038/s42003-025-07535-9 |
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