3D cell aggregates amplify diffusion signals.
Biophysical models can predict the behavior of cell cultures including 3D cell aggregates (3DCAs), thereby reducing the need for costly and time-consuming experiments. Specifically, mass transfer models enable studying the transport of nutrients, oxygen, signaling molecules, and drugs in 3DCA. These...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0310109 |
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| _version_ | 1849332029636739072 |
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| author | Hamidreza Arjmandi Kajsa P Kanebratt Liisa Vilén Peter Gennemark Adam Noel |
| author_facet | Hamidreza Arjmandi Kajsa P Kanebratt Liisa Vilén Peter Gennemark Adam Noel |
| author_sort | Hamidreza Arjmandi |
| collection | DOAJ |
| description | Biophysical models can predict the behavior of cell cultures including 3D cell aggregates (3DCAs), thereby reducing the need for costly and time-consuming experiments. Specifically, mass transfer models enable studying the transport of nutrients, oxygen, signaling molecules, and drugs in 3DCA. These models require the defining of boundary conditions (BC) between the 3DCA and surrounding medium. However, accurately modeling the BC that relates the inner and outer boundary concentrations at the border between the 3DCA and the medium remains a challenge that this paper addresses using both theoretical and experimental methods. The provided biophysical analysis indicates that the concentration of molecules inside boundary is higher than that at the outer boundary, revealing an amplification factor that is confirmed by a particle-based simulator (PBS). Due to the amplification factor, the PBS confirms that when a 3DCA with a low concentration of target molecules is introduced to a culture medium with a higher concentration, the molecule concentration in the medium rapidly decreases. The theoretical model and PBS simulations were used to design a pilot experiment with liver spheroids as the 3DCA and glucose as the target molecule. Experimental results agree with the proposed theory and derived properties. |
| format | Article |
| id | doaj-art-4f5e20695cf04ccf861df8eab4c47d29 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4f5e20695cf04ccf861df8eab4c47d292025-08-20T03:46:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01199e031010910.1371/journal.pone.03101093D cell aggregates amplify diffusion signals.Hamidreza ArjmandiKajsa P KanebrattLiisa VilénPeter GennemarkAdam NoelBiophysical models can predict the behavior of cell cultures including 3D cell aggregates (3DCAs), thereby reducing the need for costly and time-consuming experiments. Specifically, mass transfer models enable studying the transport of nutrients, oxygen, signaling molecules, and drugs in 3DCA. These models require the defining of boundary conditions (BC) between the 3DCA and surrounding medium. However, accurately modeling the BC that relates the inner and outer boundary concentrations at the border between the 3DCA and the medium remains a challenge that this paper addresses using both theoretical and experimental methods. The provided biophysical analysis indicates that the concentration of molecules inside boundary is higher than that at the outer boundary, revealing an amplification factor that is confirmed by a particle-based simulator (PBS). Due to the amplification factor, the PBS confirms that when a 3DCA with a low concentration of target molecules is introduced to a culture medium with a higher concentration, the molecule concentration in the medium rapidly decreases. The theoretical model and PBS simulations were used to design a pilot experiment with liver spheroids as the 3DCA and glucose as the target molecule. Experimental results agree with the proposed theory and derived properties.https://doi.org/10.1371/journal.pone.0310109 |
| spellingShingle | Hamidreza Arjmandi Kajsa P Kanebratt Liisa Vilén Peter Gennemark Adam Noel 3D cell aggregates amplify diffusion signals. PLoS ONE |
| title | 3D cell aggregates amplify diffusion signals. |
| title_full | 3D cell aggregates amplify diffusion signals. |
| title_fullStr | 3D cell aggregates amplify diffusion signals. |
| title_full_unstemmed | 3D cell aggregates amplify diffusion signals. |
| title_short | 3D cell aggregates amplify diffusion signals. |
| title_sort | 3d cell aggregates amplify diffusion signals |
| url | https://doi.org/10.1371/journal.pone.0310109 |
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