Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus <i>Halopseudomonas</i>
Many species of the genus <i>Pseudomonas</i> are known to be highly tolerant to solvents and other environmental stressors. Based on phylogenomic and comparative genomic analyses, several <i>Pseudomonas</i> species were recently transferred to a new genus named <i>Halop...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/12/11/2116 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850227113773236224 |
|---|---|
| author | Simone Bertoldi Pedro D. M. A. S. Mattos Carla C. C. R. de Carvalho Luzie Kruse Stephan Thies Hermann J. Heipieper Christian Eberlein |
| author_facet | Simone Bertoldi Pedro D. M. A. S. Mattos Carla C. C. R. de Carvalho Luzie Kruse Stephan Thies Hermann J. Heipieper Christian Eberlein |
| author_sort | Simone Bertoldi |
| collection | DOAJ |
| description | Many species of the genus <i>Pseudomonas</i> are known to be highly tolerant to solvents and other environmental stressors. Based on phylogenomic and comparative genomic analyses, several <i>Pseudomonas</i> species were recently transferred to a new genus named <i>Halopseudomonas</i>. Because of their unique enzymatic machinery, these strains are being discussed as novel biocatalysts in biotechnology. In order to test their growth parameters and stress tolerance, five <i>Halopseudomonas</i> strains were assessed regarding their tolerance toward different <i>n</i>-alkanols (1-butanol, 1-hexanol, 1-octanol, 1-decanol), as well as to salt stress and elevated temperatures. The toxicity of the solvents was investigated by their effects on bacterial growth rates and presented as EC50 concentrations. Hereby, all <i>Halopseudomonas</i> strains showed EC50 values up to two-fold lower than those previously detected for <i>Pseudomonas putida</i>. In addition, the activity of the <i>cis-trans</i> isomerase of unsaturated fatty acids (Cti), which is an urgent stress response mechanism known to be present in all <i>Pseudomonas</i> species, was monitored in the five <i>Halopseudomonas</i> strains. Although several of the tested species were known to contain the <i>cti</i> gene, no significant phenotypic activity could be detected in the presence of the assayed stressors. A bioinformatic analysis of eight <i>cti</i>-carrying <i>Halopseudomonas</i> strains examining promotor binding sites, binding motifs and signal peptides showed that most of the <i>cti</i> genes have a lipoprotein signal peptide and promotor regions and binding motifs that do not coincide with those of <i>Pseudomonas</i>. These insights represent putative reasons for the absence of the expected Cti activity in <i>Halopseudomonas</i>, which in turn has always been observed in <i>cti</i>-carrying <i>Pseudomonas</i>. The lack of Cti activity under membrane stress conditions when the <i>cti</i> gene is present has never been documented, and this could represent potential negative implications on the utility of the genus <i>Halopseudomonas</i> for some biotechnological applications. |
| format | Article |
| id | doaj-art-4f585ce4af4243faa0a7e8cd1ff15581 |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-4f585ce4af4243faa0a7e8cd1ff155812025-08-20T02:04:55ZengMDPI AGMicroorganisms2076-26072024-10-011211211610.3390/microorganisms12112116Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus <i>Halopseudomonas</i>Simone Bertoldi0Pedro D. M. A. S. Mattos1Carla C. C. R. de Carvalho2Luzie Kruse3Stephan Thies4Hermann J. Heipieper5Christian Eberlein6Department of Molecular Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyDepartment of Molecular Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyDepartment of Bioengineering, iBB—Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, PortugalInstitute of Molecular Enzyme Technology, Faculty of Mathematics and Natural Sciences, Heinrich Heine University Düsseldorf, 40204 Düsseldorf, GermanyInstitute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, 52428 Jülich, GermanyDepartment of Molecular Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyDepartment of Molecular Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyMany species of the genus <i>Pseudomonas</i> are known to be highly tolerant to solvents and other environmental stressors. Based on phylogenomic and comparative genomic analyses, several <i>Pseudomonas</i> species were recently transferred to a new genus named <i>Halopseudomonas</i>. Because of their unique enzymatic machinery, these strains are being discussed as novel biocatalysts in biotechnology. In order to test their growth parameters and stress tolerance, five <i>Halopseudomonas</i> strains were assessed regarding their tolerance toward different <i>n</i>-alkanols (1-butanol, 1-hexanol, 1-octanol, 1-decanol), as well as to salt stress and elevated temperatures. The toxicity of the solvents was investigated by their effects on bacterial growth rates and presented as EC50 concentrations. Hereby, all <i>Halopseudomonas</i> strains showed EC50 values up to two-fold lower than those previously detected for <i>Pseudomonas putida</i>. In addition, the activity of the <i>cis-trans</i> isomerase of unsaturated fatty acids (Cti), which is an urgent stress response mechanism known to be present in all <i>Pseudomonas</i> species, was monitored in the five <i>Halopseudomonas</i> strains. Although several of the tested species were known to contain the <i>cti</i> gene, no significant phenotypic activity could be detected in the presence of the assayed stressors. A bioinformatic analysis of eight <i>cti</i>-carrying <i>Halopseudomonas</i> strains examining promotor binding sites, binding motifs and signal peptides showed that most of the <i>cti</i> genes have a lipoprotein signal peptide and promotor regions and binding motifs that do not coincide with those of <i>Pseudomonas</i>. These insights represent putative reasons for the absence of the expected Cti activity in <i>Halopseudomonas</i>, which in turn has always been observed in <i>cti</i>-carrying <i>Pseudomonas</i>. The lack of Cti activity under membrane stress conditions when the <i>cti</i> gene is present has never been documented, and this could represent potential negative implications on the utility of the genus <i>Halopseudomonas</i> for some biotechnological applications.https://www.mdpi.com/2076-2607/12/11/2116<i>Halopseudomonas</i><i>cis-trans</i> isomerasesolvent tolerance<i>Pseudomonas</i> |
| spellingShingle | Simone Bertoldi Pedro D. M. A. S. Mattos Carla C. C. R. de Carvalho Luzie Kruse Stephan Thies Hermann J. Heipieper Christian Eberlein Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus <i>Halopseudomonas</i> Microorganisms <i>Halopseudomonas</i> <i>cis-trans</i> isomerase solvent tolerance <i>Pseudomonas</i> |
| title | Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus <i>Halopseudomonas</i> |
| title_full | Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus <i>Halopseudomonas</i> |
| title_fullStr | Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus <i>Halopseudomonas</i> |
| title_full_unstemmed | Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus <i>Halopseudomonas</i> |
| title_short | Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus <i>Halopseudomonas</i> |
| title_sort | evaluation of the robustness under alkanol stress and adaptability of members of the new genus i halopseudomonas i |
| topic | <i>Halopseudomonas</i> <i>cis-trans</i> isomerase solvent tolerance <i>Pseudomonas</i> |
| url | https://www.mdpi.com/2076-2607/12/11/2116 |
| work_keys_str_mv | AT simonebertoldi evaluationoftherobustnessunderalkanolstressandadaptabilityofmembersofthenewgenusihalopseudomonasi AT pedrodmasmattos evaluationoftherobustnessunderalkanolstressandadaptabilityofmembersofthenewgenusihalopseudomonasi AT carlaccrdecarvalho evaluationoftherobustnessunderalkanolstressandadaptabilityofmembersofthenewgenusihalopseudomonasi AT luziekruse evaluationoftherobustnessunderalkanolstressandadaptabilityofmembersofthenewgenusihalopseudomonasi AT stephanthies evaluationoftherobustnessunderalkanolstressandadaptabilityofmembersofthenewgenusihalopseudomonasi AT hermannjheipieper evaluationoftherobustnessunderalkanolstressandadaptabilityofmembersofthenewgenusihalopseudomonasi AT christianeberlein evaluationoftherobustnessunderalkanolstressandadaptabilityofmembersofthenewgenusihalopseudomonasi |