Pomegranate-peel-chitosan-gelatin composite: A hemostatic dental sponge with antibacterial enhancement
Background: Effective post-tooth extraction bleeding management and the alleviation of patient discomfort hinge upon the careful choice and judicious application of suitable hemostatic agents. Purpose: In this study, we developed a biodegradable, porous hemostatic sponge composed of gelatin (GE) and...
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Universitas Airlangga
2025-06-01
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| Series: | Dental Journal |
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| Online Access: | https://e-journal.unair.ac.id/MKG/article/view/51779 |
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| author | Lale Rozykulyyeva Prihartini Widiyanti Suryani Dyah Astuti |
| author_facet | Lale Rozykulyyeva Prihartini Widiyanti Suryani Dyah Astuti |
| author_sort | Lale Rozykulyyeva |
| collection | DOAJ |
| description | Background: Effective post-tooth extraction bleeding management and the alleviation of patient discomfort hinge upon the careful choice and judicious application of suitable hemostatic agents. Purpose: In this study, we developed a biodegradable, porous hemostatic sponge composed of gelatin (GE) and chitosan (CS), enhanced by the incorporation of pomegranate peel extract (PE), which was designed for use in dental applications, with a focus on antibacterial properties and infection prevention. Methods: The sponge was synthesized using an environmentally friendly (green) foaming approach without a foaming agent and was fabricated by freeze-drying. The efficiency of the hemostatic sponge was evaluated using various tests, including structural analysis, mechanical strength, water absorption capacity, hydrophilicity, blood clotting time (BCT), in vitro antibacterial effectiveness, and biodegradability. Results: The calcium chloride–crosslinked CS-GE and PE-immersed (CS-GE-PE) sponges exhibited adequate tensile strengths, with CS-GE-PE at 0.776 ± 0.025 MPa. The CS-GE-PE sponge showed significant water absorption (927.1% ± 37.55%). Hydrophilicity was evident (contact angle: 45°) and decreased slightly with the addition of PE. The BCT was shorter for the CS-GE sponge (161 ± 9.644 s), and both sponges exhibited minimal hemolysis, indicating biocompatibility. The CS-GE-PE sponge exhibited slightly enhanced antibacterial properties. Conclusion: This study has successfully developed a composite sponge consisting of CS, GE, and PE that exhibits a balanced level of biodegradability, antibacterial and anti-inflammatory properties, and blood absorption properties that reduce clotting time. This innovative material has great potential for a wide range of clinical applications in dental procedures and wound care. |
| format | Article |
| id | doaj-art-4f4da51f33e04cc4a4c3d6a288e4f308 |
| institution | Kabale University |
| issn | 1978-3728 2442-9740 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Dental Journal |
| spelling | doaj-art-4f4da51f33e04cc4a4c3d6a288e4f3082025-08-20T03:24:32ZengUniversitas AirlanggaDental Journal1978-37282442-97402025-06-0158217117910.20473/j.djmkg.v58.i2.p171-17949939Pomegranate-peel-chitosan-gelatin composite: A hemostatic dental sponge with antibacterial enhancementLale Rozykulyyeva0https://orcid.org/0000-0002-7170-9610Prihartini Widiyanti1https://orcid.org/0000-0002-5636-5100Suryani Dyah Astuti2https://orcid.org/0000-0003-3000-0792Department of Mathematics and Natural Sciences, Faculty of Science and Technology, Universitas Airlangga, SurabayaDepartment of Biomedical Engineering, Faculty of Science and Technology, Universitas Airlangga, SurabayaDepartment of Biomedical Engineering, Faculty of Science and Technology, Universitas Airlangga, SurabayaBackground: Effective post-tooth extraction bleeding management and the alleviation of patient discomfort hinge upon the careful choice and judicious application of suitable hemostatic agents. Purpose: In this study, we developed a biodegradable, porous hemostatic sponge composed of gelatin (GE) and chitosan (CS), enhanced by the incorporation of pomegranate peel extract (PE), which was designed for use in dental applications, with a focus on antibacterial properties and infection prevention. Methods: The sponge was synthesized using an environmentally friendly (green) foaming approach without a foaming agent and was fabricated by freeze-drying. The efficiency of the hemostatic sponge was evaluated using various tests, including structural analysis, mechanical strength, water absorption capacity, hydrophilicity, blood clotting time (BCT), in vitro antibacterial effectiveness, and biodegradability. Results: The calcium chloride–crosslinked CS-GE and PE-immersed (CS-GE-PE) sponges exhibited adequate tensile strengths, with CS-GE-PE at 0.776 ± 0.025 MPa. The CS-GE-PE sponge showed significant water absorption (927.1% ± 37.55%). Hydrophilicity was evident (contact angle: 45°) and decreased slightly with the addition of PE. The BCT was shorter for the CS-GE sponge (161 ± 9.644 s), and both sponges exhibited minimal hemolysis, indicating biocompatibility. The CS-GE-PE sponge exhibited slightly enhanced antibacterial properties. Conclusion: This study has successfully developed a composite sponge consisting of CS, GE, and PE that exhibits a balanced level of biodegradability, antibacterial and anti-inflammatory properties, and blood absorption properties that reduce clotting time. This innovative material has great potential for a wide range of clinical applications in dental procedures and wound care.https://e-journal.unair.ac.id/MKG/article/view/51779antibacterialdentistrygelatinhemostatic effectmedicine |
| spellingShingle | Lale Rozykulyyeva Prihartini Widiyanti Suryani Dyah Astuti Pomegranate-peel-chitosan-gelatin composite: A hemostatic dental sponge with antibacterial enhancement Dental Journal antibacterial dentistry gelatin hemostatic effect medicine |
| title | Pomegranate-peel-chitosan-gelatin composite: A hemostatic dental sponge with antibacterial enhancement |
| title_full | Pomegranate-peel-chitosan-gelatin composite: A hemostatic dental sponge with antibacterial enhancement |
| title_fullStr | Pomegranate-peel-chitosan-gelatin composite: A hemostatic dental sponge with antibacterial enhancement |
| title_full_unstemmed | Pomegranate-peel-chitosan-gelatin composite: A hemostatic dental sponge with antibacterial enhancement |
| title_short | Pomegranate-peel-chitosan-gelatin composite: A hemostatic dental sponge with antibacterial enhancement |
| title_sort | pomegranate peel chitosan gelatin composite a hemostatic dental sponge with antibacterial enhancement |
| topic | antibacterial dentistry gelatin hemostatic effect medicine |
| url | https://e-journal.unair.ac.id/MKG/article/view/51779 |
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