Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy
Ergothioneine is a natural amino acid with powerful antioxidant activity. Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans, and enhance telomerase activity. In present study, the metagenomics sequencing and quantita...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2023.9250028 |
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| author | Hongyu Pan Qianwang Zheng Yuan Zou Guozhao Luo Mingzhen Tu Na Wang Jingru Zhong Liqiong Guo Junfang Lin |
| author_facet | Hongyu Pan Qianwang Zheng Yuan Zou Guozhao Luo Mingzhen Tu Na Wang Jingru Zhong Liqiong Guo Junfang Lin |
| author_sort | Hongyu Pan |
| collection | DOAJ |
| description | Ergothioneine is a natural amino acid with powerful antioxidant activity. Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans, and enhance telomerase activity. In present study, the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies. And the alterations in the gut barrier, gut morphology, and other gut physiological indicators of aging flies were evaluated. Here, the results showed that ergothioneine induced little variations in gut microbiota composition in flies at mid-life, and significant alterations in the gut microbiota populations were only observed in flies at late -life. And ergothioneine attenuated age-related gut injuries, including decreasing reactive oxygen species levels in intestinal enterocytes, reduction of intestinal cells death, and preservation of gut integrity in aging flies. Ergothioneine also improved the gut morphology by preserving the structure and shape of cell organelles and inducing the intestinal autophagy. And activation of intestinal autophagy also contributed to the longevity effect mediated by ergothioneine in an unidentified mechanism. |
| format | Article |
| id | doaj-art-4f4b3c862b554a29b0f1561072fd3a11 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-4f4b3c862b554a29b0f1561072fd3a112025-01-10T06:57:02ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-11-011363434344610.26599/FSHW.2023.9250028Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagyHongyu Pan0Qianwang Zheng1Yuan Zou2Guozhao Luo3Mingzhen Tu4Na Wang5Jingru Zhong6Liqiong Guo7Junfang Lin8College of Food Science, South China Agricultural University, Guangzhou 510640, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510640, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510640, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510640, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510640, ChinaGuangzhou Alchemy Biotechnology Co., Ltd., Guangzhou 510760, ChinaGuangzhou Alchemy Biotechnology Co., Ltd., Guangzhou 510760, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510640, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510640, ChinaErgothioneine is a natural amino acid with powerful antioxidant activity. Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans, and enhance telomerase activity. In present study, the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies. And the alterations in the gut barrier, gut morphology, and other gut physiological indicators of aging flies were evaluated. Here, the results showed that ergothioneine induced little variations in gut microbiota composition in flies at mid-life, and significant alterations in the gut microbiota populations were only observed in flies at late -life. And ergothioneine attenuated age-related gut injuries, including decreasing reactive oxygen species levels in intestinal enterocytes, reduction of intestinal cells death, and preservation of gut integrity in aging flies. Ergothioneine also improved the gut morphology by preserving the structure and shape of cell organelles and inducing the intestinal autophagy. And activation of intestinal autophagy also contributed to the longevity effect mediated by ergothioneine in an unidentified mechanism.https://www.sciopen.com/article/10.26599/FSHW.2023.9250028ergothioneinegut microbiotaintestinal autophagyintestinal barrierdrosophila melanogaster |
| spellingShingle | Hongyu Pan Qianwang Zheng Yuan Zou Guozhao Luo Mingzhen Tu Na Wang Jingru Zhong Liqiong Guo Junfang Lin Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy Food Science and Human Wellness ergothioneine gut microbiota intestinal autophagy intestinal barrier drosophila melanogaster |
| title | Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy |
| title_full | Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy |
| title_fullStr | Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy |
| title_full_unstemmed | Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy |
| title_short | Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy |
| title_sort | geroprotection from ergothioneine treatment in drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy |
| topic | ergothioneine gut microbiota intestinal autophagy intestinal barrier drosophila melanogaster |
| url | https://www.sciopen.com/article/10.26599/FSHW.2023.9250028 |
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