Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis

Campylobacter upsaliensis was the most common Campylobacter species in pets’ gastrointestinal tracts and has been isolated from patients with bacteremia, hemolytic-uremic syndrome, spontaneous abortion, and Guillain-Barré syndrome. However, the genetic characteristics and the full extent of its sign...

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Main Authors: Hairui Wang, Yixin Gu, Changyan Ju, Ying Li, Xiaoli Chen, Guilan Zhou, Xin Zhang, Chang Liu, Jing Chen, Yue Han, Jianzhong Zhang, Zhujun Shao, Maojun Zhang
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Emerging Microbes and Infections
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Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2023.2294857
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author Hairui Wang
Yixin Gu
Changyan Ju
Ying Li
Xiaoli Chen
Guilan Zhou
Xin Zhang
Chang Liu
Jing Chen
Yue Han
Jianzhong Zhang
Zhujun Shao
Maojun Zhang
author_facet Hairui Wang
Yixin Gu
Changyan Ju
Ying Li
Xiaoli Chen
Guilan Zhou
Xin Zhang
Chang Liu
Jing Chen
Yue Han
Jianzhong Zhang
Zhujun Shao
Maojun Zhang
author_sort Hairui Wang
collection DOAJ
description Campylobacter upsaliensis was the most common Campylobacter species in pets’ gastrointestinal tracts and has been isolated from patients with bacteremia, hemolytic-uremic syndrome, spontaneous abortion, and Guillain-Barré syndrome. However, the genetic characteristics and the full extent of its significance as a human pathogen remain to be fully understood. This study involved an investigation for genomic analysis of 154 strains from different sources and additional antimicrobial resistance profiles of 26 strains for this species. The genomes contained 1,558–1,971 CDS and the genome sizes were estimated to vary from 1.53 Mb to 1.86 Mb, with an average GC content of  34.71%. The entire analyzed genomes could be divided into three clades (A, B, and C) based on ANI and phylogenomic analysis. Significantly, nearly all strains in Clade B were isolated from patient samples, and the virulence-related sequences FlgD, GmhA, and CdtC might serve as determining factors for the classification of Clade B. Half of the tested isolates had MIC values over 64 μg mL−1 for nalidixic acid, gentamicin, and streptomycin. Isolates from pets in China carried more resistant elements in the genomes. This study both provided a comprehensive profile of C. upsaliensis for its genomic features and suggested some pathogenic agents for human infection with this species.
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spelling doaj-art-4f49ff179d2b4fcea67dbe38b4341af42025-08-20T02:37:32ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512024-12-0113110.1080/22221751.2023.2294857Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysisHairui Wang0Yixin Gu1Changyan Ju2Ying Li3Xiaoli Chen4Guilan Zhou5Xin Zhang6Chang Liu7Jing Chen8Yue Han9Jianzhong Zhang10Zhujun Shao11Maojun Zhang12National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaNanshan Center for Disease Control and Prevention, Shenzhen, People’s Republic of ChinaShunyi District Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaMeilianzhonghe Veterinary Hospital Companion Branch, Beijing, People’s Republic of ChinaMeilianzhonghe Veterinary Hospital Jingxi Branch, Beijing, People’s Republic of ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaCampylobacter upsaliensis was the most common Campylobacter species in pets’ gastrointestinal tracts and has been isolated from patients with bacteremia, hemolytic-uremic syndrome, spontaneous abortion, and Guillain-Barré syndrome. However, the genetic characteristics and the full extent of its significance as a human pathogen remain to be fully understood. This study involved an investigation for genomic analysis of 154 strains from different sources and additional antimicrobial resistance profiles of 26 strains for this species. The genomes contained 1,558–1,971 CDS and the genome sizes were estimated to vary from 1.53 Mb to 1.86 Mb, with an average GC content of  34.71%. The entire analyzed genomes could be divided into three clades (A, B, and C) based on ANI and phylogenomic analysis. Significantly, nearly all strains in Clade B were isolated from patient samples, and the virulence-related sequences FlgD, GmhA, and CdtC might serve as determining factors for the classification of Clade B. Half of the tested isolates had MIC values over 64 μg mL−1 for nalidixic acid, gentamicin, and streptomycin. Isolates from pets in China carried more resistant elements in the genomes. This study both provided a comprehensive profile of C. upsaliensis for its genomic features and suggested some pathogenic agents for human infection with this species.https://www.tandfonline.com/doi/10.1080/22221751.2023.2294857Campylobacter upsaliensispathogenic agentcomparative genomicsvirulence factorantimicrobial resistance
spellingShingle Hairui Wang
Yixin Gu
Changyan Ju
Ying Li
Xiaoli Chen
Guilan Zhou
Xin Zhang
Chang Liu
Jing Chen
Yue Han
Jianzhong Zhang
Zhujun Shao
Maojun Zhang
Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis
Emerging Microbes and Infections
Campylobacter upsaliensis
pathogenic agent
comparative genomics
virulence factor
antimicrobial resistance
title Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis
title_full Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis
title_fullStr Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis
title_full_unstemmed Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis
title_short Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis
title_sort genetic characteristics and potential pathogenic agents in campylobacter upsaliensis based on genomic analysis
topic Campylobacter upsaliensis
pathogenic agent
comparative genomics
virulence factor
antimicrobial resistance
url https://www.tandfonline.com/doi/10.1080/22221751.2023.2294857
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