Conformity Test of Maldi Biotyper Culture Identification Results with Maldi Biotyper Sepsityper® IVD Kit from Cerebrospinal Fluid Specimens in Bacterial Meningitis: A Preliminary Study
AIM: The study assessed the conformity of using the Sepsityper® IVD MBT kit with MALDI Biotyper (MBT) from colonies on solid media to identify pathogens from cerebrospinal fluid (CSF) specimens in positive blood culture bottles. BACKGROUND: The identification of microorganisms by mass spectrometry,...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524002868 |
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| Summary: | AIM: The study assessed the conformity of using the Sepsityper® IVD MBT kit with MALDI Biotyper (MBT) from colonies on solid media to identify pathogens from cerebrospinal fluid (CSF) specimens in positive blood culture bottles. BACKGROUND: The identification of microorganisms by mass spectrometry, such as MBT, has been used on colonies grown on solid media. Unfortunately, infections mainly caused by fastidious bacteria often do not grow on solid media and require a longer identification time. Direct identification of positive blood culture bottles with the Sepsityper® IVD MBT kit allows for the identification of microorganisms until species level in a maximum of 15-20 minutes without inoculation on solid media. It is necessary to evaluate the use of the Sepsityper® IVD MBT Kit on CSF and observe its identification suitability with the MBT method from colonies in solid media. METHODS: This is a cross-sectional study. CSF specimens grown in blood culture bottles were directly identified using the Sepsityper® IVD MBT kit. The results were then compared with identification using MBTs from colonies grown on solid media. The conformity of the results is calculated by referring to the Kappa value. RESULTS: From 10 CSF specimens, we found that identifying pathogens using Sepsityper® IVD MBT Kit is 100% identical to the MBT method of colonies on solid media (Cohen's Kappa 1). CONCLUSIONS: The results showed the consistency of pathogen identification between Sepsityper® IVD MBT Kit and MBT. Although this study is preliminary, Sepsityper® IVD MBT shows promising results for routine laboratory examinations. |
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| ISSN: | 2213-7165 |