Seasonality and Vertical Structure of Microbial Communities in Alpine Wetlands
The soil microbial community plays a crucial role in the elemental cycling and energy flow within wetland ecosystems. The temporal dynamics and spatial distribution of soil microbial communities are central topics in ecology. While numerous studies have focused on wetland microbial community structu...
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2025-04-01
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| author | Huiyuan Wang Yue Li Xiaoqin Yang Bin Niu Hongzhe Jiao Ya Yang Guoqiang Huang Weiguo Hou Gengxin Zhang |
| author_facet | Huiyuan Wang Yue Li Xiaoqin Yang Bin Niu Hongzhe Jiao Ya Yang Guoqiang Huang Weiguo Hou Gengxin Zhang |
| author_sort | Huiyuan Wang |
| collection | DOAJ |
| description | The soil microbial community plays a crucial role in the elemental cycling and energy flow within wetland ecosystems. The temporal dynamics and spatial distribution of soil microbial communities are central topics in ecology. While numerous studies have focused on wetland microbial community structures at low altitudes, microbial diversity across seasons and depths and their environmental determinants remain poorly understudied. To test the seasonal variation in microbial communities with contrasting seasonal fluxes of greenhouse gases, a total of 36 soil samples were collected from different depths in the Namco wetland on the Tibetan Plateau across four seasons. We found significant seasonal variation in bacterial community composition, most pronounced in the Winter, but not in archaea. In particular, Proteobacteria decreased by 11.5% in Winter compared with other seasons (<i>p</i> < 0.05). The bacterial alpha diversity showed hump-shaped seasonal patterns with lower diversity in Winter, whereas archaea showed no significant patterns across depths. A PERMANOVA further revealed significant differences in the bacterial community structure between Winter and the other three seasons (<i>p</i> < 0.05). In addition, bacterial and archaeal community structures differed between surface (0–5 cm) and deeper (5–30 cm) soils (<i>p</i> < 0.01). Redundancy analysis showed that soil total nitrogen, soil total phosphorus, and total soil organic carbon significantly influenced bacteria and archaea (<i>p</i> < 0.05). Furthermore, soil moisture content and temperature strongly affected the bacterial community structure (<i>p</i> < 0.001). Our findings highlighted the seasonal variation in the microbial community and the profound influence of soil moisture and temperature on microbial structure in alpine wetlands on the Tibetan Plateau. |
| format | Article |
| id | doaj-art-4f33ab74051b43efa7f97deaed2ae158 |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-4f33ab74051b43efa7f97deaed2ae1582025-08-20T02:33:47ZengMDPI AGMicroorganisms2076-26072025-04-0113596210.3390/microorganisms13050962Seasonality and Vertical Structure of Microbial Communities in Alpine WetlandsHuiyuan Wang0Yue Li1Xiaoqin Yang2Bin Niu3Hongzhe Jiao4Ya Yang5Guoqiang Huang6Weiguo Hou7Gengxin Zhang8Institute of Earth Sciences, China University of Geosciences, Beijing 100083, ChinaInstitute of Earth Sciences, China University of Geosciences, Beijing 100083, ChinaInstitute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Earth Sciences, China University of Geosciences, Beijing 100083, ChinaInstitute of Earth Sciences, China University of Geosciences, Beijing 100083, ChinaInstitute of Earth Sciences, China University of Geosciences, Beijing 100083, ChinaInstitute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, ChinaThe soil microbial community plays a crucial role in the elemental cycling and energy flow within wetland ecosystems. The temporal dynamics and spatial distribution of soil microbial communities are central topics in ecology. While numerous studies have focused on wetland microbial community structures at low altitudes, microbial diversity across seasons and depths and their environmental determinants remain poorly understudied. To test the seasonal variation in microbial communities with contrasting seasonal fluxes of greenhouse gases, a total of 36 soil samples were collected from different depths in the Namco wetland on the Tibetan Plateau across four seasons. We found significant seasonal variation in bacterial community composition, most pronounced in the Winter, but not in archaea. In particular, Proteobacteria decreased by 11.5% in Winter compared with other seasons (<i>p</i> < 0.05). The bacterial alpha diversity showed hump-shaped seasonal patterns with lower diversity in Winter, whereas archaea showed no significant patterns across depths. A PERMANOVA further revealed significant differences in the bacterial community structure between Winter and the other three seasons (<i>p</i> < 0.05). In addition, bacterial and archaeal community structures differed between surface (0–5 cm) and deeper (5–30 cm) soils (<i>p</i> < 0.01). Redundancy analysis showed that soil total nitrogen, soil total phosphorus, and total soil organic carbon significantly influenced bacteria and archaea (<i>p</i> < 0.05). Furthermore, soil moisture content and temperature strongly affected the bacterial community structure (<i>p</i> < 0.001). Our findings highlighted the seasonal variation in the microbial community and the profound influence of soil moisture and temperature on microbial structure in alpine wetlands on the Tibetan Plateau.https://www.mdpi.com/2076-2607/13/5/962alpine wetlandsdepthseasonal variationsoil microbes |
| spellingShingle | Huiyuan Wang Yue Li Xiaoqin Yang Bin Niu Hongzhe Jiao Ya Yang Guoqiang Huang Weiguo Hou Gengxin Zhang Seasonality and Vertical Structure of Microbial Communities in Alpine Wetlands Microorganisms alpine wetlands depth seasonal variation soil microbes |
| title | Seasonality and Vertical Structure of Microbial Communities in Alpine Wetlands |
| title_full | Seasonality and Vertical Structure of Microbial Communities in Alpine Wetlands |
| title_fullStr | Seasonality and Vertical Structure of Microbial Communities in Alpine Wetlands |
| title_full_unstemmed | Seasonality and Vertical Structure of Microbial Communities in Alpine Wetlands |
| title_short | Seasonality and Vertical Structure of Microbial Communities in Alpine Wetlands |
| title_sort | seasonality and vertical structure of microbial communities in alpine wetlands |
| topic | alpine wetlands depth seasonal variation soil microbes |
| url | https://www.mdpi.com/2076-2607/13/5/962 |
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