Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes.
<h4>Background</h4>Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114947&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850190659139403776 |
|---|---|
| author | Yen T Duong Reshma Kassanjee Alex Welte Meade Morgan Anindya De Trudy Dobbs Erin Rottinghaus John Nkengasong Marcel E Curlin Chonticha Kittinunvorakoon Boonyos Raengsakulrach Michael Martin Kachit Choopanya Suphak Vanichseni Yan Jiang Maofeng Qiu Haiying Yu Yan Hao Neha Shah Linh-Vi Le Andrea A Kim Tuan Anh Nguyen William Ampofo Bharat S Parekh |
| author_facet | Yen T Duong Reshma Kassanjee Alex Welte Meade Morgan Anindya De Trudy Dobbs Erin Rottinghaus John Nkengasong Marcel E Curlin Chonticha Kittinunvorakoon Boonyos Raengsakulrach Michael Martin Kachit Choopanya Suphak Vanichseni Yan Jiang Maofeng Qiu Haiying Yu Yan Hao Neha Shah Linh-Vi Le Andrea A Kim Tuan Anh Nguyen William Ampofo Bharat S Parekh |
| author_sort | Yen T Duong |
| collection | DOAJ |
| description | <h4>Background</h4>Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estimated previously required recalibration. We present here results of recalibration efforts using >250 seroconversion panels and multiple statistical methods to ensure accuracy and consensus.<h4>Methods</h4>A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs.<h4>Results</h4>Using different statistical methods, MDRI values ranged from 88-94 days at cutoff ODn = 1.0 to 177-183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing for misclassification among long-term infections indicated that overall PFRs were 0.6% to 2.5% at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR (<2.0%) suggests that a cutoff ODn = 1.5, corresponding to an MDRI of 130 days should be used for cross-sectional application. The MDRI varied among subtypes from 109 days (subtype A&D) to 152 days (subtype C).<h4>Conclusions</h4>Based on the new data and revised analysis, we recommend an ODn cutoff = 1.5 to classify recent and long-term infections, corresponding to an MDRI of 130 days (118-142). Determination of revised parameters for estimation of HIV-1 incidence should facilitate application of the LAg-Avidity EIA for worldwide use. |
| format | Article |
| id | doaj-art-4f2d89bad83a4cd59dfa76418ff200cc |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4f2d89bad83a4cd59dfa76418ff200cc2025-08-20T02:15:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011494710.1371/journal.pone.0114947Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes.Yen T DuongReshma KassanjeeAlex WelteMeade MorganAnindya DeTrudy DobbsErin RottinghausJohn NkengasongMarcel E CurlinChonticha KittinunvorakoonBoonyos RaengsakulrachMichael MartinKachit ChoopanyaSuphak VanichseniYan JiangMaofeng QiuHaiying YuYan HaoNeha ShahLinh-Vi LeAndrea A KimTuan Anh NguyenWilliam AmpofoBharat S Parekh<h4>Background</h4>Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estimated previously required recalibration. We present here results of recalibration efforts using >250 seroconversion panels and multiple statistical methods to ensure accuracy and consensus.<h4>Methods</h4>A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs.<h4>Results</h4>Using different statistical methods, MDRI values ranged from 88-94 days at cutoff ODn = 1.0 to 177-183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing for misclassification among long-term infections indicated that overall PFRs were 0.6% to 2.5% at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR (<2.0%) suggests that a cutoff ODn = 1.5, corresponding to an MDRI of 130 days should be used for cross-sectional application. The MDRI varied among subtypes from 109 days (subtype A&D) to 152 days (subtype C).<h4>Conclusions</h4>Based on the new data and revised analysis, we recommend an ODn cutoff = 1.5 to classify recent and long-term infections, corresponding to an MDRI of 130 days (118-142). Determination of revised parameters for estimation of HIV-1 incidence should facilitate application of the LAg-Avidity EIA for worldwide use.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114947&type=printable |
| spellingShingle | Yen T Duong Reshma Kassanjee Alex Welte Meade Morgan Anindya De Trudy Dobbs Erin Rottinghaus John Nkengasong Marcel E Curlin Chonticha Kittinunvorakoon Boonyos Raengsakulrach Michael Martin Kachit Choopanya Suphak Vanichseni Yan Jiang Maofeng Qiu Haiying Yu Yan Hao Neha Shah Linh-Vi Le Andrea A Kim Tuan Anh Nguyen William Ampofo Bharat S Parekh Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. PLoS ONE |
| title | Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. |
| title_full | Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. |
| title_fullStr | Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. |
| title_full_unstemmed | Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. |
| title_short | Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. |
| title_sort | recalibration of the limiting antigen avidity eia to determine mean duration of recent infection in divergent hiv 1 subtypes |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114947&type=printable |
| work_keys_str_mv | AT yentduong recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT reshmakassanjee recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT alexwelte recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT meademorgan recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT anindyade recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT trudydobbs recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT erinrottinghaus recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT johnnkengasong recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT marcelecurlin recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT chontichakittinunvorakoon recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT boonyosraengsakulrach recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT michaelmartin recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT kachitchoopanya recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT suphakvanichseni recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT yanjiang recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT maofengqiu recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT haiyingyu recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT yanhao recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT nehashah recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT linhvile recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT andreaakim recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT tuananhnguyen recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT williamampofo recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes AT bharatsparekh recalibrationofthelimitingantigenavidityeiatodeterminemeandurationofrecentinfectionindivergenthiv1subtypes |